BBa_K1949052 1 BBa_K1949052 PBAD-rbs-amiE 2016-10-05T11:00:00Z 2016-10-14T09:13:40Z AmiE is derived from artificially synthesized DNA. This gene codes for protein AmiE. AmiE is an acylase that is capable of degrading long chain N-acyl homoserine lactone (AHL) molecules with acyl chains longer than six carbons. false false _2416_ 31979 31979 9 false We applied a site-specific mutation to the amiE in order to destroy a recognition site of EcoRI. We changed the 291th base A to G. false Yoshio Takata component2487204 1 BBa_I0500 component2487206 1 BBa_B0034 component2487207 1 BBa_K1949050 annotation2487204 1 BBa_I0500 range2487204 1 1 1210 annotation2487206 1 BBa_B0034 range2487206 1 1219 1230 annotation2487207 1 BBa_K1949050 range2487207 1 1237 2712 BBa_I0500 1 pBad/araC Inducible pBad/araC promoter 2003-12-05T12:00:00Z 2015-08-31T04:07:29Z -- No description -- false true _1_ 0 24 7 In stock false true Sri Kosuri annotation1498843 1 AraC range1498843 1 1 1079 annotation1498842 1 PC range1498842 1 1043 1114 annotation1498841 1 Pbad range1498841 1 1080 1210 BBa_K1949050 1 BBa_K1949050 amiE 2016-10-05T11:00:00Z 2016-10-08T07:25:13Z This gene was isolated from Acinetobacter sp. strain Ooi24. fwd: rev: This gene codes for protein AmiE. AmiE is an acylase that is capable of degrading long chain N-acyl homoserine lactone (AHL) molecules with acyl chains longer than six carbons. false false _2416_ 32067 31979 9 false sequence confirmed false Yoshio Takata BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1949052_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagatgccaaaagattttgtttatgatgatgcaactgcgttagtacaatccatccaaaatggacaaatcagtagccgtgaattgctggaatcacagattgaacggattaataaatacaacaaacacatcaatagttgtgttgcatttgattatgacaatgcaaggattttagcagatcaggctgacctcgccatcaagcgacaagccccacttggtaaattacatggcattccgatgaccatcaaagatgcctttaaagtgaaaggcatgccgtgtaccgatggcaatccagagttccaacactatgtccccaccgaaaatgctgtttcggtcaataaattggtcggtgctggagccattccttttgcaaaaaccaatgtaccctttaaatgtgcagatattcaaacctacaacaaattttatggtactgccaataatccatggaatttaaatctcacaacaggcggctcatcaggtggttctgctgcggcactagcatcgggatttacaccgattgagcttggtagtgatattggtggttctattcgaacaccagcacatttttgtggtgtctatggtcataaatccacctatggattgattgatttcagaggacacatcatgaattacgaggacgagttatcccaacctgaccttgccgtcattggtccaatggctcgttcagcacgggacttaagtttattattagatgttttagtcgagccaaatgcggatgactttagagcttttaaactcaaggaatctgaaaaacaaaccattcaagagtttaaagtattattctggatggatgatgaatcgtgtccaatcgattcacggctaaagaaaaaatacgatcaacttttgcaaaccctgcaaactgccaaggttaacgtggatgtaggtcgtccaaaaaattgggattttgatgaaatatttaaatcctacgccactcgattgatcagccaaatgacttttgcagatcctaaactaagtcgattgtccatgtcggcatctacgccattaatcaaattacttaatggtaaagcaggcattccgcctttggcctatggatttacacaaggtgctaacctgagtcatgcagaatggcttgccaaatatgaagagtcattgcatatcaagcaaaaatgtcttgaaatttttgcagaatacgacgtcattatttgcccacctattttgactctagcgtttgaacacaatcataaagaacctttgatgtttcgcacgctacaagtcgatggaagaaaacgtttctatcttgagttattcaaatggatttcgccagcaactgtatttgggttgccatcaacctctgcaccgataggcttatctgaggacaatctaccagtcaatattcaaatattgggacaaccgtatgcagacaaagtcaccattaaatttgctgaactgcttgcacaaattacggatggattccaaaaaccgcctttggatttttaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_I0500_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc BBa_K1949050_sequence 1 atgccaaaagattttgtttatgatgatgcaactgcgttagtacaatccatccaaaatggacaaatcagtagccgtgaattgctggaatcacagattgaacggattaataaatacaacaaacacatcaatagttgtgttgcatttgattatgacaatgcaaggattttagcagatcaggctgacctcgccatcaagcgacaagccccacttggtaaattacatggcattccgatgaccatcaaagatgcctttaaagtgaaaggcatgccgtgtaccgatggcaatccagagttccaacactatgtccccaccgaaaatgctgtttcggtcaataaattggtcggtgctggagccattccttttgcaaaaaccaatgtaccctttaaatgtgcagatattcaaacctacaacaaattttatggtactgccaataatccatggaatttaaatctcacaacaggcggctcatcaggtggttctgctgcggcactagcatcgggatttacaccgattgagcttggtagtgatattggtggttctattcgaacaccagcacatttttgtggtgtctatggtcataaatccacctatggattgattgatttcagaggacacatcatgaattacgaggacgagttatcccaacctgaccttgccgtcattggtccaatggctcgttcagcacgggacttaagtttattattagatgttttagtcgagccaaatgcggatgactttagagcttttaaactcaaggaatctgaaaaacaaaccattcaagagtttaaagtattattctggatggatgatgaatcgtgtccaatcgattcacggctaaagaaaaaatacgatcaacttttgcaaaccctgcaaactgccaaggttaacgtggatgtaggtcgtccaaaaaattgggattttgatgaaatatttaaatcctacgccactcgattgatcagccaaatgacttttgcagatcctaaactaagtcgattgtccatgtcggcatctacgccattaatcaaattacttaatggtaaagcaggcattccgcctttggcctatggatttacacaaggtgctaacctgagtcatgcagaatggcttgccaaatatgaagagtcattgcatatcaagcaaaaatgtcttgaaatttttgcagaatacgacgtcattatttgcccacctattttgactctagcgtttgaacacaatcataaagaacctttgatgtttcgcacgctacaagtcgatggaagaaaacgtttctatcttgagttattcaaatggatttcgccagcaactgtatttgggttgccatcaacctctgcaccgataggcttatctgaggacaatctaccagtcaatattcaaatattgggacaaccgtatgcagacaaagtcaccattaaatttgctgaactgcttgcacaaattacggatggattccaaaaaccgcctttggatttttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z