LOCUS       BBa_K1949102            2520 bp    DNA     linear       28-Jun-2024
DEFINITION  PBAD-rbs-mazF-tt-Ptet-rbs-gfp
ACCESSION   BBa_K1949102
VERSION     BBa_K1949102.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1575
                     /label=protein
     CDS             1237..1575
                     /label=BBa_K1096002
     stem_loop       1595..1638
                     /label=stem_loop
     misc_feature    1584..1663
                     /label=BBa_B0010
     terminator      1584..1663
                     /label=BBa_B0010
     stem_loop       1679..1698
                     /label=T7 TE
     misc_feature    1705..1705
                     /label=stop
     polyA_site      1699..1712
                     /label=polya
     misc_feature    1672..1712
                     /label=BBa_B0012
     terminator      1672..1712
                     /label=BBa_B0012
     terminator      1584..1712
                     /label=BBa_B0015
     misc_feature    1721..1739
                     /label=TetR 1
     misc_feature    1721..1774
                     /label=BBa_R0040
     promoter        1740..1745
                     /label=-35
     misc_feature    1746..1764
                     /label=TetR 2
     promoter        1763..1768
                     /label=-10
     promoter        1721..1774
                     /label=BBa_R0040
     misc_feature    1787..1790
                     /label=conserved
     RBS             1783..1794
                     /label=BBa_B0034
     CDS             1801..2520
                     /label=GFP protein
     CDS             1801..2520
                     /label=BBa_E0040
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgg taagccgata cgtacccgat
     1261 atgggcgatc tgatttgggt tgattttgac ccgacaaaag gtagcgagca agctggacat
     1321 cgtccagctg ttgtcctgag tcctttcatg tacaacaaca aaacaggtat gtgtctgtgt
     1381 gttccttgta caacgcaatc aaaaggatat ccgttcgaag ttgttttatc cggtcaggaa
     1441 cgtgatggcg tagcgttagc tgatcaggta aaaagtatcg cctggcgggc aagaggagca
     1501 acgaagaaag gaacagttgc cccagaggaa ttacaactca ttaaagccaa aattaacgta
     1561 ctgattgggt aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1621 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1681 ctcaccttcg ggtgggcctt tctgcgttta tatactagag tccctatcag tgatagagat
     1741 tgacatccct atcagtgata gagatactga gcactactag agaaagagga gaaatactag
     1801 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
     1861 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
     1921 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
     1981 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
     2041 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
     2101 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
     2161 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
     2221 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
     2281 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
     2341 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     2401 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
     2461 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa
//