LOCUS       BBa_K1950010            1946 bp    DNA     linear       22-Nov-2024
DEFINITION  trc promoter + ispC-ribB(G108S) fusion
ACCESSION   BBa_K1950010
VERSION     BBa_K1950010.1  
FEATURES             Location/Qualifiers
     promoter        1..56
                     /label=trc promoter
     RBS             63..66
                     /label=RBS
     promoter        1..74
                     /label=BBa_K1950000
     misc_feature    227..227
                     /label=C to T mutation
     CDS             81..1946
                     /label=BBa_K1950006
ORIGIN
        1 ttgacaatta atcatccggc tcgtataatg tgtggaattg tgagcggata acaatttcac
       61 acaggaaaca gacctactag atgaagcaac tcaccattct gggctcgacc ggctcgattg
      121 gttgcagcac gctggacgtg gtgcgccata atcccgaaca cttccgcgta gttgcgctgg
      181 tggcaggcaa aaatgtcact cgcatggtag aacagtgcct ggaattttct ccccgctatg
      241 ccgtaatgga cgatgaagcg agtgcgaaac ttcttaaaac gatgctacag caacagggta
      301 gccgcaccga agtcttaagt gggcaacaag ccgcttgcga tatggcagcg cttgaggatg
      361 ttgatcaggt gatggcagcc attgttggcg ctgctgggct gttacctacg cttgctgcga
      421 tccgcgcggg taaaaccatt ttgctggcca ataaagaatc actggttacc tgcggacgtc
      481 tgtttatgga cgccgtaaag cagagcaaag cgcaattgtt accggtcgat agcgaacata
      541 acgccatttt tcagagttta ccgcaaccta tccagcataa tctgggatac gctgaccttg
      601 agcaaaatgg cgtggtgtcc attttactta ccgggtctgg tggccctttc cgtgagacgc
      661 cattgcgcga tttggcaaca atgacgccgg atcaagcctg ccgtcatccg aactggtcga
      721 tggggcgtaa aatttctgtc gattcggcta ccatgatgaa caaaggtctg gaatacattg
      781 aagcgcgttg gctgtttaac gccagcgcca gccagatgga agtgctgatt cacccgcagt
      841 cagtgattca ctcaatggtg cgctatcagg acggcagtgt tctggcgcag ctgggggaac
      901 cggatatgcg tacgccaatt gcccacacca tggcatggcc gaatcgcgtg aactctggcg
      961 tgaagccgct cgatttttgc aaactaagtg cgttgacatt tgccgcaccg gattatgatc
     1021 gttatccatg cctgaaactg gcgatggagg cgttcgaaca aggccaggca gcgacgacag
     1081 cattgaatgc cgcaaacgaa atcaccgttg ctgcttttct tgcgcaacaa atccgcttta
     1141 cggatatcgc tgcgttgaat ttatccgtac tggaaaaaat ggatatgcgc gaaccacaat
     1201 gtgtggacga tgtgttatct gttgatgcga acgcgcgtga agtcgccaga aaagaggtga
     1261 tgcgtctcgc aagcggttct ggcggttccg gtgcaaatca gacgctactt tcctcttttg
     1321 gtacgccttt cgaacgtgtt gaaaatgcac tggctgcgct gcgtgaagga cgcggtgtaa
     1381 tggtgcttga tgatgaagac cgtgaaaacg aaggtgatat gatcttcccg gcagaaacca
     1441 tgactgttga gcagatggcg ctgaccattc gccacggtag cggtattgtt tgcctgtgca
     1501 ttactgaaga tcgccgtaaa caactcgatc tgccaatgat ggtagaaaat aacaccagcg
     1561 cctatggcac cggttttacc gtgaccattg aagcagctga aggtgtgact accagcgttt
     1621 ctgccgctga ccgtattacg accgttcgcg cagcgattgc cgatggcgca aaaccgtcag
     1681 atctgaatcg tcctggccac gttttcccac ttcgcgctca ggcaggtggt gtactgacgc
     1741 gtggcggtca tactgaagca actattgatc tgatgacgct ggcaggcttt aaaccggctg
     1801 gtgtactgtg tgagctgact aatgacgatg gcacgatggc gcgtgcacca gagtgtattg
     1861 agtttgccaa taaacacaat atggcgctcg tgactattga agacctggtg gcataccgtc
     1921 aggcacatga gcgtaaagcc agctga
//