BBa_K1951011
type: ComponentDefinition
persistent identity: BBa K1951011
display id: BBa_K1951011
version: 1
was generated by: iGEM to SBOL conversion
description: desABCD cluster producing biosynthesis pathway to make Desferrioxamine B
created: 2016-10-12T11:00:00Z
modified: 2016-10-15T02:18:05Z
mutable provenance: We used 7 previous biobricks to design this one : - BBa_B0034 - BBa_I0500 - BBa_K1951000 - BBa_K1951001 - BBa_K1951002 - BBa_K1951003
owned by: james
owned by: myers
mutable description: Desferrioxamine B is a siderophore produced in Streptomyces coelicolor to catch vital irons on the extracellular medium. In consequence, this molecule which have a greatly high affinity for iron is secreted in the external medium and bring back inside the bacteria full of iron mol??cules. Previous work showed that siderophore which are molecule secreted by bacteria to catch iron are able to catch other metals by default. Especifically, many articles showed a high affinity of Desferrioxamine B ( produce by ''Streptomyces coelicolor'') for tetravalent metal ions and more specifically platinum. These results encouraged us to make a biobrick coding the sequence corresponding to the 4 enzymes involved on the metabolic pathway of Desferrioxamine B. ''E. coli'' was used to produce this biobrick. Produce a gram positive bacteria pathway in a gram negative bacteria is restrictive, considering the risk of toxicity for the conductress bacteria. To counter this potential issue, we regulate trasnscription using the control of an inductible promotor (pBAD/araC). Our strategy for the siderophore mobilisation results in two main steps : 1. Transformation of E.Coli with an inductible plasmide holding the ''des'' cluster 2. Induction and production of the desferrioxamine B
discontinued: false
dominant: false
group u list: _2418_
m user id: 31894
owner id: 31894
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: There is no any design consideration for this biobrick.
star: false
creator: claire raynaud
type: DnaRegion
role: partType/DNA
role: SO:0000110
component: BBa_K1951003
component: BBa_K1951002
component: BBa_K1951001
component: BBa_K1951000
component: BBa_B0034
component: BBa_I0500
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1951000
sequence annotation: BBa_K1951001
sequence annotation: BBa_K1951002
sequence annotation: BBa_K1951003
sequence: https://synbiohub.org/public/igem/BBa_K1951011_sequence/1

BBa_K1951003
type: Component
persistent identity: component2512497
display id: component2512497
version: 1
top level: BBa_K1951011
definition: BBa_K1951003
access: public

BBa_K1951002
type: Component
persistent identity: component2512494
display id: component2512494
version: 1
top level: BBa_K1951011
definition: BBa_K1951002
access: public

BBa_K1951001
type: Component
persistent identity: component2512491
display id: component2512491
version: 1
top level: BBa_K1951011
definition: BBa_K1951001
access: public

BBa_K1951000
type: Component
persistent identity: component2512488
display id: component2512488
version: 1
top level: BBa_K1951011
definition: BBa_K1951000
access: public

BBa_B0034
type: Component
persistent identity: component2512485
display id: component2512485
version: 1
top level: BBa_K1951011
definition: BBa_B0034
access: public

BBa_I0500
type: Component
persistent identity: component2512483
display id: component2512483
version: 1
top level: BBa_K1951011
definition: pBad/araC
access: public

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2512483
display id: annotation2512483
version: 1
top level: BBa_K1951011
location: https://synbiohub.org/public/igem/BBa_K1951011/annotation2512483/range2512483/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1951011/annotation2512483/range2512483/1
type: Range
persistent identity: range2512483
display id: range2512483
version: 1
top level: BBa_K1951011
direction: direction/reverse
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2512485
display id: annotation2512485
version: 1
top level: BBa_K1951011
location: https://synbiohub.org/public/igem/BBa_K1951011/annotation2512485/range2512485/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1951011/annotation2512485/range2512485/1
type: Range
persistent identity: range2512485
display id: range2512485
version: 1
top level: BBa_K1951011
direction: direction/reverse
start: 1219
end: 1230
orientation: inline

BBa_K1951000
type: SequenceAnnotation
persistent identity: annotation2512488
display id: annotation2512488
version: 1
top level: BBa_K1951011
location: https://synbiohub.org/public/igem/BBa_K1951011/annotation2512488/range2512488/1
component: BBa_K1951000

https://synbiohub.org/public/igem/BBa_K1951011/annotation2512488/range2512488/1
type: Range
persistent identity: range2512488
display id: range2512488
version: 1
top level: BBa_K1951011
direction: direction/reverse
start: 1237
end: 2682
orientation: inline

BBa_K1951001
type: SequenceAnnotation
persistent identity: annotation2512491
display id: annotation2512491
version: 1
top level: BBa_K1951011
location: https://synbiohub.org/public/igem/BBa_K1951011/annotation2512491/range2512491/1
component: BBa_K1951001

https://synbiohub.org/public/igem/BBa_K1951011/annotation2512491/range2512491/1
type: Range
persistent identity: range2512491
display id: range2512491
version: 1
top level: BBa_K1951011
direction: direction/reverse
start: 2689
end: 3933
orientation: inline

BBa_K1951002
type: SequenceAnnotation
persistent identity: annotation2512494
display id: annotation2512494
version: 1
top level: BBa_K1951011
location: https://synbiohub.org/public/igem/BBa_K1951011/annotation2512494/range2512494/1
component: BBa_K1951002

https://synbiohub.org/public/igem/BBa_K1951011/annotation2512494/range2512494/1
type: Range
persistent identity: range2512494
display id: range2512494
version: 1
top level: BBa_K1951011
direction: direction/reverse
start: 3940
end: 4497
orientation: inline

BBa_K1951003
type: SequenceAnnotation
persistent identity: annotation2512497
display id: annotation2512497
version: 1
top level: BBa_K1951011
location: https://synbiohub.org/public/igem/BBa_K1951011/annotation2512497/range2512497/1
component: BBa_K1951003

https://synbiohub.org/public/igem/BBa_K1951011/annotation2512497/range2512497/1
type: Range
persistent identity: range2512497
display id: range2512497
version: 1
top level: BBa_K1951011
direction: direction/reverse
start: 4504
end: 6294
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

pBad/araC
type: ComponentDefinition
persistent identity: BBa I0500
display id: BBa_I0500
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible pBad/araC promoter
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sri Kosuri
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: AraC
sequence annotation: PC
sequence annotation: Pbad
sequence: https://synbiohub.org/public/igem/BBa_I0500_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation1498843
display id: annotation1498843
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
type: Range
persistent identity: range1498843
display id: range1498843
version: 1
top level: pBad/araC
direction: direction/forward
start: 1
end: 1079
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation1498842
display id: annotation1498842
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
type: Range
persistent identity: range1498842
display id: range1498842
version: 1
top level: pBad/araC
direction: direction/forward
start: 1043
end: 1114
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation1498841
display id: annotation1498841
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
type: Range
persistent identity: range1498841
display id: range1498841
version: 1
top level: pBad/araC
direction: direction/forward
start: 1080
end: 1210
orientation: inline

BBa_K1951000
type: ComponentDefinition
persistent identity: BBa K1951000
display id: BBa_K1951000
version: 1
was generated by: iGEM to SBOL conversion
description: desA, lysine decarboxylase coding sequence
created: 2016-10-09T11:00:00Z
modified: 2016-10-11T05:44:37Z
mutable provenance: This part comes from the genome sequencing of Streptomyces coelicolor. The following website enabled to find the gene reference and its chromosomal position. http://strepdb.streptomyces.org.uk/cgi-bin/dc3.pl?accession=AL645882&serial=2769&width=900&start=3031362&end=3041362&iorm=map The second website below is the source of the DNA sequence we have used. http://www.ncbi.nlm.nih.gov/nuccore/NC_003888.3
owned by: james
owned by: myers
mutable description: This DNA sequence codes Lysine descarboxylase (Streptomyces coelicolor) is an enzyme from the lyase family that converts lysine to cadaverin. The enzyme realizes the carbonyl group of the lysin amino acid. There is cadeverin producing (1,5-diaminopentane), a primary diamine which alkaline environment. The lysine decarboxylase is an enzyme induced the synthesis of which is promoted by anaerobiosis and an acidic pH. In bacteriology, this enzyme is sought through the middle of Moeller lysine or medium lysine Taylor. This enzyme is also the first step in the production of desferrioxame B which is a siderophore.
discontinued: false
dominant: false
experience: experience/None
group u list: _2418_
m user id: 31894
owner id: 31894
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The DNA sequence was modified by the addition of a prefix and suffix sequences (containing the restriction site EcoRI, XbaI and SpeI, PstI respectively) and was inserted in a promoteur using SLIC method.
star: false
creator: Claire raynaud, Yoann Santin, Louis Givelet
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: desA
sequence annotation: double stop
sequence: https://synbiohub.org/public/igem/BBa_K1951000_sequence/1

desA
type: SequenceAnnotation
persistent identity: annotation2491880
display id: annotation2491880
version: 1
top level: BBa_K1951000
location: https://synbiohub.org/public/igem/BBa_K1951000/annotation2491880/range2491880/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1951000/annotation2491880/range2491880/1
type: Range
persistent identity: range2491880
display id: range2491880
version: 1
top level: BBa_K1951000
direction: direction/forward
start: 1
end: 1440
orientation: inline

double stop
type: SequenceAnnotation
persistent identity: annotation2491881
display id: annotation2491881
version: 1
top level: BBa_K1951000
location: https://synbiohub.org/public/igem/BBa_K1951000/annotation2491881/range2491881/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1951000/annotation2491881/range2491881/1
type: Range
persistent identity: range2491881
display id: range2491881
version: 1
top level: BBa_K1951000
direction: direction/forward
start: 1440
end: 1446
orientation: inline

BBa_K1951001
type: ComponentDefinition
persistent identity: BBa K1951001
display id: BBa_K1951001
version: 1
was generated by: iGEM to SBOL conversion
description: des B, monooxygenase coding sequence
created: 2016-10-09T11:00:00Z
modified: 2016-10-11T06:00:24Z
mutable provenance: The following website contains the gene reference and its chromosomal location. http://strepdb.streptomyces.org.uk/cgi-bin/dc3.pl?accession=AL645882&serial=2769&width=900&start=3031362&end=3041362&iorm=map Start : 3037106 End : 3038347 The coding sequence of DNA comes from http://www.ncbi.nlm.nih.gov/nuccore/NC_003888.3
owned by: james
owned by: myers
mutable description: This sequence codes Monooxygenase which is an enzyme that incorporate one hydroxyl group into substrates in many metabolic pathways. In this reaction, the two atoms of dioxygen are reduced to one hydroxyl and one H2O molecule by the concomitant oxidation of NAD(P)H. It is also the second step in the production of desferrioxamine B in Streptomyces coelicolor, allowing the transformation of cadeverine into N-hydroxycadaverine.
discontinued: false
dominant: false
group u list: _2418_
m user id: 31894
owner id: 31894
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We had prefix and suffix to the DNA sequence containing restriction sites (EcoRI, Xba1 and SpeI, PstI respectively.
star: false
creator: claire raynaud
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: DesB
sequence annotation: double stop
sequence: https://synbiohub.org/public/igem/BBa_K1951001_sequence/1

DesB
type: SequenceAnnotation
persistent identity: annotation2491892
display id: annotation2491892
version: 1
top level: BBa_K1951001
location: https://synbiohub.org/public/igem/BBa_K1951001/annotation2491892/range2491892/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1951001/annotation2491892/range2491892/1
type: Range
persistent identity: range2491892
display id: range2491892
version: 1
top level: BBa_K1951001
direction: direction/forward
start: 1
end: 1245
orientation: inline

double stop
type: SequenceAnnotation
persistent identity: annotation2491893
display id: annotation2491893
version: 1
top level: BBa_K1951001
location: https://synbiohub.org/public/igem/BBa_K1951001/annotation2491893/range2491893/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1951001/annotation2491893/range2491893/1
type: Range
persistent identity: range2491893
display id: range2491893
version: 1
top level: BBa_K1951001
direction: direction/forward
start: 1240
end: 1245
orientation: inline

BBa_K1951002
type: ComponentDefinition
persistent identity: BBa K1951002
display id: BBa_K1951002
version: 1
was generated by: iGEM to SBOL conversion
description: desC, acyl transferase coding sequence
created: 2016-10-09T11:00:00Z
modified: 2016-10-11T05:46:37Z
mutable provenance: The following website gave us information about the gene reference and his chromosomal location. http://strepdb.streptomyces.org.uk/cgi-bin/dc3.pl?accession=AL645882&serial=2769&width=900&start=3031362&end=3041362&iorm=map Start : 3038344 End : 3038898 The coding sequence ised comes from the website below : http://www.ncbi.nlm.nih.gov/nuccore/NC_003888.3
owned by: james
owned by: myers
mutable description: desC is the coding sequence of DesC which is an acyl transferase of the Desferrioxamine production pathway. It is the enzyme of the third step that transform N-hydroxycadaverin into N-acetyl N-hydroxucadaverine by an Acetyl-CoA dependent manner.
discontinued: false
dominant: false
group u list: _2418_
m user id: 31894
owner id: 31894
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We added prefix and suffix sequences containing the restriction sites (EcoR1, XbaI and SpeI, PstI respectively)
star: false
creator: claire raynaud
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: desC
sequence annotation: double stop
sequence: https://synbiohub.org/public/igem/BBa_K1951002_sequence/1

desC
type: SequenceAnnotation
persistent identity: annotation2491886
display id: annotation2491886
version: 1
top level: BBa_K1951002
location: https://synbiohub.org/public/igem/BBa_K1951002/annotation2491886/range2491886/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1951002/annotation2491886/range2491886/1
type: Range
persistent identity: range2491886
display id: range2491886
version: 1
top level: BBa_K1951002
direction: direction/forward
start: 1
end: 558
orientation: inline

double stop
type: SequenceAnnotation
persistent identity: annotation2491887
display id: annotation2491887
version: 1
top level: BBa_K1951002
location: https://synbiohub.org/public/igem/BBa_K1951002/annotation2491887/range2491887/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1951002/annotation2491887/range2491887/1
type: Range
persistent identity: range2491887
display id: range2491887
version: 1
top level: BBa_K1951002
direction: direction/forward
start: 555
end: 558
orientation: inline

BBa_K1951003
type: ComponentDefinition
persistent identity: BBa K1951003
display id: BBa_K1951003
version: 1
was generated by: iGEM to SBOL conversion
description: desD, desferrioxamine biosynthesis protein coding sequence
created: 2016-10-09T11:00:00Z
modified: 2016-10-11T06:02:25Z
mutable provenance: Gene reference and its chromosomal position have been found on the following website : http://strepdb.streptomyces.org.uk/cgi-bin/dc3.pl?accession=AL645882&serial=2769&width=900&start=3031362&end=3041362&iorm=map Start : 3038895 End : 3040682 The website bellows is the source of the DNA sequence we used. http://www.ncbi.nlm.nih.gov/nuccore/NC_003888.3
owned by: james
owned by: myers
mutable description: This coding sequence codes DesD od Streptomyces coelicolor, which is the last protein involved in the metabolic pathway of Desferrioxamine B. This enzyme is called desferrioxamine biosynthesis protein or DesD and allows to transform N-acetyl N-hydroxycadaverine into Desferrioxamine B by the transformation of 3a nucleoside triphosphate into 3a nucleoside 5'monophosphate-3-diphosphate.
discontinued: false
dominant: false
group u list: _2418_
m user id: 31894
owner id: 31894
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We added prefix and suffix subsequences (containing EcoRI, XbaI and SpeI, PstI restriction sites respectively).
star: false
creator: claire raynaud
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: desD
sequence annotation: double stop
sequence: https://synbiohub.org/public/igem/BBa_K1951003_sequence/1

desD
type: SequenceAnnotation
persistent identity: annotation2491923
display id: annotation2491923
version: 1
top level: BBa_K1951003
location: https://synbiohub.org/public/igem/BBa_K1951003/annotation2491923/range2491923/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1951003/annotation2491923/range2491923/1
type: Range
persistent identity: range2491923
display id: range2491923
version: 1
top level: BBa_K1951003
direction: direction/forward
start: 1
end: 1791
orientation: inline

double stop
type: SequenceAnnotation
persistent identity: annotation2491924
display id: annotation2491924
version: 1
top level: BBa_K1951003
location: https://synbiohub.org/public/igem/BBa_K1951003/annotation2491924/range2491924/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1951003/annotation2491924/range2491924/1
type: Range
persistent identity: range2491924
display id: range2491924
version: 1
top level: BBa_K1951003
direction: direction/forward
start: 1787
end: 1791
orientation: inline

https://synbiohub.org/public/igem/BBa_K1951011_sequence/1
type: Sequence
persistent identity: BBa K1951011 sequence
display id: BBa_K1951011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagatgcgctcgcacctgctcaacgacaccaccgcggagcagtaccgccgttccgtgaccgaaggagtcgagcgggtggccgccaaactcgccaccaccgaccgcccgttcaccggcgtcacggtcgacgccctctccccccgcatcgacgcgatcgacctcgacgagccgctgcacgacaccgccgcggtcctcgacgagctggaggacgtctacctccgcgacgccgtctacttccaccacccccgctacctcgcccacctcaactgcccggtcgtcataccggcgctgctcggtgaagcggtcctgtccgccgtcaactcctccctggacacctgggaccagtcggccggcggcacgctcatcgagcggaagctgatcgactggacctgcgcccgcatcggcctcggcccggcggccgacggcgtgttcacctccggcggcacccagtccaacctccaggcgctgctcctcgcccgcgaggaggcgaaggccgaggacttcgccgacctgcggatcttcgcctccgaggccagccacttcagcgtcaggaagtccgcgaaactgctcggcctcggccccgacgccgtcgtgtcgatcccggtcgaccgcgacaagcggatgcagaccgtcgccctcgcccgcgagctggagcgctgcgcgcgggacggcctggtccccatggccgtcgtcgccaccggcggcaccaccgacttcggctcgatcgacccgctgccggagatcgccgggctgtgcgagcagtacgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0500_sequence/1
type: Sequence
persistent identity: BBa I0500 sequence
display id: BBa_I0500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1951000_sequence/1
type: Sequence
persistent identity: BBa K1951000 sequence
display id: BBa_K1951000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgctcgcacctgctcaacgacaccaccgcggagcagtaccgccgttccgtgaccgaaggagtcgagcgggtggccgccaaactcgccaccaccgaccgcccgttcaccggcgtcacggtcgacgccctctccccccgcatcgacgcgatcgacctcgacgagccgctgcacgacaccgccgcggtcctcgacgagctggaggacgtctacctccgcgacgccgtctacttccaccacccccgctacctcgcccacctcaactgcccggtcgtcataccggcgctgctcggtgaagcggtcctgtccgccgtcaactcctccctggacacctgggaccagtcggccggcggcacgctcatcgagcggaagctgatcgactggacctgcgcccgcatcggcctcggcccggcggccgacggcgtgttcacctccggcggcacccagtccaacctccaggcgctgctcctcgcccgcgaggaggcgaaggccgaggacttcgccgacctgcggatcttcgcctccgaggccagccacttcagcgtcaggaagtccgcgaaactgctcggcctcggccccgacgccgtcgtgtcgatcccggtcgaccgcgacaagcggatgcagaccgtcgccctcgcccgcgagctggagcgctgcgcgcgggacggcctggtccccatggccgtcgtcgccaccggcggcaccaccgacttcggctcgatcgacccgctgccggagatcgccgggctgtgcgagcagtacggcgtgtggatgcacgtcgacgcggcctacggctgcgggctgctcgcctccctgaagtaccgggaccgcatcaccggcatcgagcgggccgactcggtcaccgtggactaccacaagtccttcttccagccggtgagttcgtcggccgtgctggtccgggacgcggccaccctgcgccacgccacctaccacgcggagtacctcaacccgcgccgcatggtgcaggaacgtatccccaaccaggtggacaagtccctccagaccacccgccgcttcgacgcgctcaagctgtggatgacgctgcgcgtgatgggcgccgacggcatcggcgtcctcttcgacgaggtgtgcgacctggccgccgagggctggaaactgctcgccgccgacccgcgcttcgacgtcgtggtccagccgtcgctgtccacgctggtcttccgccacatcccggcggacgtcaccgatcccgccgagatcgaccgcgccaacctgtacgcccgcaaggccctgttcgcgtccggcgacgccgtggtcgcgggcaccaaggtcgccggtcgccactacctgaagttcaccctgctcaaccccgagacgaccccggccgacatcgccgccgtcctcgacctgatcgccggccacgccgagcagtacctgggagactcccttgaccgcgcttcctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1951001_sequence/1
type: Sequence
persistent identity: BBa K1951001 sequence
display id: BBa_K1951001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggcatcggcctcggccccttcaacctcggcctcgcctgcctcaccgagcccgtcgccgagctgaacggcgtcttcctggagtccaagccggacttcgagtggcacgccgggatgttcctggacggcgcccacctccagaccccgttcatgtcggacctggtgacgctcgccgacccgacctcgccgtactccttcctcaactacctgaaggagcagggccggctgtactccttctacatccgggagaacttctacccgctgcgggtcgagtacgacgactactgccgctgggccgcgcgcaagctgagcagcgtccgcttctccaccacggtcaccgaggtcacgtacgacgagcgcgaggagctgtacgccgtcgccaccacgtccggcgacacctaccgcgcccgccgcctcgtcctcggcaccggcaccccgccgcacatcccggacgcctgccggggcctggccggcgacttcctccacaactcccggtacgtgcggcaccgggcggagctggtgaagaagaagtcgatcacgctggtcggcagcggccagtccgccgccgagatctaccaggacctgctgagcgagatcgacgtccacggctacgggctgaactgggtgacccgctccccgcggttcttcccgctcgaatacaccaagctcaccctggagatgacgtccccggagtacgtggactactaccacgcgctgcccgaggacacccgctaccgcctcacggccgagcagaagggcctgttcaagggcatcgacggcgacctgatcaacgagatcttcgacctgctctaccagaaacggctcggcggccccgtccccacccgcctgctcaccaactcggcgctgaccagcgcccggtatgcggacggcacctacacgctcgggttccgccaggaggagcagggcacggacttcgagatcgagaccgagggcctggtcctcgccaccggctaccggtacaccgagccggagttcctcaagcccgtccgggaccggctgcgctacgactcccgcggcaacttcgacatcggccgcaactacgccgtcgacgtcacgggaggcggcgtgttcctccagaacgcgggggtccacgcgcacagcgtcaccagccccgacctgggcatgggcgcctaccgcaacagctgcatcgtccgggagctgctcggacgcgagtactacccggtcgagcagtcgatcgcgttccaggagttcgccgtataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1951002_sequence/1
type: Sequence
persistent identity: BBa K1951002 sequence
display id: BBa_K1951002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagccgcttgagcaccaccacccccgtcggggcactgaccctgcgccccgtcgacccgctgacggacgccgtactgctgcacggctggctcacccaccccaagtccgcgttctggatgatgcaggacgcccggctggtggacgtcgagcgggcctacatggagctggccgccgacgagcaccagcaggcccacctcggcctgcacgacggggtcccggccttcctgacggagcgctacgaccccgcccaccgcgaactggtcgggctgtacgagcccgagccgggcgacgtcggcatgcacttcctggtcgcgcccaccgaccggcccgtgcacggcttcacccgcgccgtgatcaccaccgtgatgacggagctgttcgccgacccggcgacccggcgggtcgtcgtcgaaccggacgtcaccaacaccgccgtgcacgccctgaacgcagccgtcggattcgtgcccgagcgcgagatccagaagccggagaagaaggccttgctgagcttctgcacccgcgagcagttcgcgaaggcggtgtccgcataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1951003_sequence/1
type: Sequence
persistent identity: BBa K1951003 sequence
display id: BBa_K1951003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcctcgccgacgccgtcgcccacctgacccccgaacgctgggaggaggccaaccgcctcctggtccgcaaggcgctggccgagttcacccacgagcggctgctgacccccgagcgggagccggacgacgggggcgggcagacgtacgtcgtccgcagcgacgacggccagaccgcgtaccgcttcaccgccaccgtccgcgccctggaccactggcaggtggacgccgcctccgtcacccgccaccgcgacggcgcggaactcccgctcgccgcgctggacttcttcatcgagctgaagcagaccctgggcctgagcgacgagatcctcccggtctacctggaggagatctcctccaccctctccggcacctgctacaaactgaccaagccgcagctcagctccgccgagctggcccggagcggagacttccaggccgtcgagacgggcatgaccgagggccacccgtgcttcgtcgccaacaacgggcgcctcggcttcggcatccacgagtacctgtcgtacgcccccgagaccgcgagcccggtccggctggtgtggctggccgcgcaccgctcgcgggcggcgttcacggcgggcgtgggcatcgagtacgagtccttcgtccgggacgagctgggcgcggccaccgtcgaccgcttccacggcgtgctgcgcgggcgcggcctggacccggccgactacctgctcatcccggtccacccctggcagtggtggaacaagctcaccgtcaccttcgccgccgaggtcgcccgcgggcacctggtgtgcctgggcgagggcgacgacgagtacctggcccagcagtccatccgcaccttcttcaacgcctcgcaccccgggaagcactacgtgaagactgccctgtccgtcctcaacatgggcttcatgcgcggtctgtcggcggcgtacatggaggccactccggccatcaacgactggctcgcccggctgatcgagggcgacccggtgctgaaggagacggggctgagcatcatccgggagcgggcggccgtcggctaccggcacctggagtacgagcaggccaccgaccgctactcgccctaccgcaagatgctggcggcgctgtggcgggagagcccggtgccgtccatccgggagggcgagacgctcgccaccatggcctccctggtccaccaggaccacgagggcgcctccttcgcgggcgcgctgatcgagcggtccggactcacgcccaccgagtggctgcggcactacctgcgggcctactacgtcccgctgctgcacagcttctacgcctacgacctggtgtacatgccgcacggcgagaacgtgatcctggtgctggcggacggggtggtgcggcgggcggtctacaaggacatcgccgaggagatcgcggtgatggacccggacgcggtgctgccgccggaggtctcccgcatcgcggtggacgtgccggacgacaagaagctcctgtcgatcttcacggacgtcttcgactgcttcttccgcttcctggccgcgaacctggcggaggaggggatcgtcacggaggacgccttctggcggacggtcgcggaggtcacccgggagtaccaggagtcggtgccggagctggccgacaagttcgagcggtacgacatgttcgcgcccgagttcgccctgtcctgcctcaaccggctccagctgcgcgacaaccggcagatggtggacctggccgacccgtccggcgcgctccagctcgtcggcaccctgaagaaccccctggcaggccggtaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z