LOCUS       BBa_K195104             2066 bp    DNA     linear       26-Dec-2024
DEFINITION  pTet+RBS+Lpp-OmpA-linker+CD4 gene+terminator
ACCESSION   BBa_K195104
VERSION     BBa_K195104.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    83..89
                     /label=NdeI
     CDS             86..520
                     /label=OmpA
     CDS             521..546
                     /label=linker
     misc_feature    541..546
                     /label=SacI
     misc_feature    86..88
                     /label=ATG
     misc_feature    83..546
                     /label=BBa_K103006
     CDS             553..1929
                     /label=BBa_K195100
     stem_loop       1949..1992
                     /label=stem_loop
     misc_feature    1938..2017
                     /label=BBa_B0010
     terminator      1938..2017
                     /label=BBa_B0010
     stem_loop       2033..2052
                     /label=T7 TE
     misc_feature    2059..2059
                     /label=stop
     polyA_site      2053..2066
                     /label=polya
     misc_feature    2026..2066
                     /label=BBa_B0012
     terminator      2026..2066
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag agcatatgaa agctactaaa ctggtactgg gcgcggtaat
      121 cctgggttct actctgctgg caggttgctc cagcaacgct aaaatcgatc agggaattaa
      181 cccgtatgtt ggctttgaaa tgggttacga ctggttaggt cgtatgccgt acaaaggcag
      241 cgttgaaaac ggtgcataca aagctcaggg cgttcaactg accgctaaac tgggttaccc
      301 aatcactgac gacctggaca tctacactcg tctgggtggc atggtatggc gtgcagacac
      361 taaatccaac gtttatggta aaaaccacga caccggcgtt tctccggtct tcgctggcgg
      421 tgttgagtac gcgatcactc ctgaaatcgc tacccgtctg gaataccagt ggaccaacaa
      481 catcggtgac gcacacacca tcggcactcg tccggacaac ggcggaggtt ctggaggagg
      541 gagctctact agatgaaccg gggagtccct tttaggcact tgcttctggt gctgcaactg
      601 gcgctcctcc cagcagccac tcagggaaag aaagtggtgc tgggcaaaaa aggggataca
      661 gtggaactga cctgtacagc ttcccagaag aagagcatac aattccactg gaaaaactcc
      721 aaccagataa agattctggg aaatcagggc tccttcttaa ctaaaggtcc atccaagctg
      781 aatgatcgcg ctgactcaag aagaagcctt tgggaccaag gaaactttcc cctgatcatc
      841 aagaatctta agatagaaga ctcagatact tacatctgtg aagtggagga ccagaaggag
      901 gaggtgcaat tgctagtgtt cggattgact gccaactctg acacccacct gcttcagggg
      961 cagagcctga ccctgacctt ggagagcccc cctggtagta gcccctcagt gcaatgtagg
     1021 agtccaaggg gtaaaaacat acaggggggg aagaccctct ccgtgtctca gctggagctc
     1081 caggatagtg gcacctggac atgcactgtc ttgcagaacc agaagaaggt ggagttcaaa
     1141 atagacatcg tggtgctagc tttccagaag gcctccagca tagtctataa gaaagagggg
     1201 gaacaggtgg agttctcctt cccactcgcc tttacagttg aaaagctgac gggcagtggc
     1261 gagctgtggt ggcaggcgga gagggcttcc tcctccaagt cttggatcac ctttgacctg
     1321 aagaacaagg aagtgtctgt aaaacgggtt acccaggacc ctaagctcca gatgggcaag
     1381 aagctcccgc tccacctcac cctgccccag gccttgcctc agtatgctgg ctctggaaac
     1441 ctcaccctgg cccttgaagc gaaaacagga aagttgcatc aggaagtgaa cctggtggtg
     1501 atgagagcca ctcagctcca gaaaaatttg acctgtgagg tgtggggacc cacctcccct
     1561 aagctgatgc tgagtttgaa actggagaac aaggaggcaa aggtctcgaa gcgggagaag
     1621 gcggtgtggg tgctgaaccc tgaggcgggg atgtggcagt gtctgctgag tgactcggga
     1681 caggtcctgc tggaatccaa catcaaggtt ctgcccacat ggtccacccc ggtgcagcca
     1741 atggccctga ttgtgctggg gggcgtcgcc ggcctcctgc ttttcattgg gctaggcatc
     1801 ttcttctgtg tcaggtgccg gcaccgaagg cgccaagcag agcggatgtc tcagatcaag
     1861 agactcctca gtgagaagaa gacctgccag tgtcctcacc ggtttcagaa gacatgtagc
     1921 cccatttgat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1981 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     2041 ttcgggtggg cctttctgcg tttata
//