LOCUS       BBa_K195109             1748 bp    DNA     linear       17-May-2024
DEFINITION  pTet+RBS+Lpp-OmpA-linker+ CCR5 gene+terminator
ACCESSION   BBa_K195109
VERSION     BBa_K195109.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    83..89
                     /label=NdeI
     CDS             86..520
                     /label=OmpA
     CDS             521..546
                     /label=linker
     misc_feature    541..546
                     /label=SacI
     misc_feature    86..88
                     /label=ATG
     misc_feature    83..546
                     /label=BBa_K103006
     CDS             553..1611
                     /label=BBa_K195105
     stem_loop       1631..1674
                     /label=stem_loop
     misc_feature    1620..1699
                     /label=BBa_B0010
     terminator      1620..1699
                     /label=BBa_B0010
     stem_loop       1715..1734
                     /label=T7 TE
     misc_feature    1741..1741
                     /label=stop
     polyA_site      1735..1748
                     /label=polya
     misc_feature    1708..1748
                     /label=BBa_B0012
     terminator      1708..1748
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag agcatatgaa agctactaaa ctggtactgg gcgcggtaat
      121 cctgggttct actctgctgg caggttgctc cagcaacgct aaaatcgatc agggaattaa
      181 cccgtatgtt ggctttgaaa tgggttacga ctggttaggt cgtatgccgt acaaaggcag
      241 cgttgaaaac ggtgcataca aagctcaggg cgttcaactg accgctaaac tgggttaccc
      301 aatcactgac gacctggaca tctacactcg tctgggtggc atggtatggc gtgcagacac
      361 taaatccaac gtttatggta aaaaccacga caccggcgtt tctccggtct tcgctggcgg
      421 tgttgagtac gcgatcactc ctgaaatcgc tacccgtctg gaataccagt ggaccaacaa
      481 catcggtgac gcacacacca tcggcactcg tccggacaac ggcggaggtt ctggaggagg
      541 gagctctact agatggatta tcaagtgtca agtccaatct atgacatcaa ttattataca
      601 tcggagccct gccaaaaaat caatgtgaag caaatcgcag cccgcctcct gcctccgctc
      661 tactcactgg tgttcatctt tggttttgtg ggcaacatgc tggtcatcct catcctgata
      721 aactgcaaaa ggctgaagag catgactgac atctacctgc tcaacctggc catctctgac
      781 ctgtttttcc ttcttactgt ccccttctgg gctcactatg ctgccgccca gtgggacttt
      841 ggaaatacaa tgtgtcaact cttgacaggg ctctatttta taggcttctt ctctggaatc
      901 ttcttcatca tcctcctgac aatcgatagg tacctggctg tcgtccatgc tgtgtttgct
      961 ttaaaagcca ggacggtcac ctttggggtg gtgacaagtg tgatcacttg ggtggtggct
     1021 gtgtttgcgt ctctcccagg aatcatcttt accagatctc aaaaagaagg tcttcattac
     1081 acctgcagct ctcattttcc atacagtcag tatcaattct ggaagaattt ccagacatta
     1141 aagatagtca tcttggggct ggtcctgccg ctgcttgtca tggtcatctg ctactcggga
     1201 atcctaaaaa ctctgcttcg gtgtcgaaat gagaagaaga ggcacagggc tgtgaggctt
     1261 atcttcacca tcatgattgt ttattttctc ttctgggctc cctacaacat tgtccttctc
     1321 ctgaacacct tccaggaatt ctttggcctg aataattgca gtagctctaa caggttggac
     1381 caagctatgc aggtgacaga gactcttggg atgacgcact gctgcatcaa ccccatcatc
     1441 tatgcctttg tcggggagaa gttcagaaac tacctcttag tcttcttcca aaagcacatt
     1501 gccaaacgct tctgcaaatg ctgttctatt ttccagcaag aggctcccga gcgagcaagc
     1561 tcagtttaca cccgatccac tggggagcag gaaatatctg tgggcttgtg atactagagc
     1621 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1681 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1741 cgtttata
//