BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation2001 1 lac O1 range2001 1 26 42 annotation1999 1 lac O1 range1999 1 3 19 annotation2002 1 -10 range2002 1 43 48 annotation7064 1 BBa_R0011 range7064 1 1 54 annotation2000 1 -35 range2000 1 20 25 BBa_K1952005 1 BBa_K1952005 Hydrazine Synthase subunit alpha (Kust-2861) with strong RBS 2016-10-18T11:00:00Z 2016-10-19T04:49:16Z Taken from iGEM parts B0034 and K1952002 Protein coding region with translation initiation signals, to be used alone or in operon false false _2419_ 32346 32346 9 false None false Jessica Zhao component2522942 1 BBa_K1952002 component2522941 1 BBa_B0034 annotation2522941 1 BBa_B0034 range2522941 1 1 12 annotation2522942 1 BBa_K1952002 range2522942 1 19 2445 BBa_K1952002 1 BBa_K1952002 Hydrazine Synthase alpha subunit 2016-10-13T11:00:00Z 2016-10-29T09:32:31Z hydrazine synthase subunit hydrazine synthase subunit false false _2419_ 26856 33370 9 false hydrazine synthase subunit false Alex Dean BBa_K1952008 1 BBa_K1952008 Hydrazine Synthase subunit alpa (Kust-2861) with strong RBS under Lac promoter control 2016-10-18T11:00:00Z 2016-10-29T09:58:02Z Hydrazine Synthase subunit alpa (Kust-2861) with strong RBS Protein coding region with translation initiation signals, to be used alone or in operon false false _2419_ 26856 32346 9 false none false Jessica Zhao component2522976 1 BBa_R0011 component2522984 1 BBa_K1952005 annotation2522984 1 BBa_K1952005 range2522984 1 64 2508 annotation2522976 1 BBa_R0011 range2522976 1 1 54 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1952002_sequence 1 atgggcaaacgcaaactgggcgtgattgcgagcgcgtttgtggcgggcgcgctggtgtgcggcagcaccctggtgaacgcggaaccggtgatgaccggcggcccggtgcagggcaaagcgctgtggaccgattatagcggcatgagcaaagaagtacagggtccagtgagccagattctgtttacccagagcccgcgcaccgcgaaaggcgatccgtatcagaactatccgcattatattccggaaggcagccgcattgtgctgtttgatctgaacaccaaagaactgaaagtgctgaccaacgattttgcgaccgccttcgacccatgcacctattgggatggcaaaaaatttgcgtttgcgggcgtgcataaaaaaggcggcggctgccaaatctgggaaatgaacattgatggcagcggcctgcgccagatgactgacctcaaaggcacctgccgcagcccgatttattatgcggcgggcagcattgaagaaggcgaaggccgaatcatttggcgcgatcgctattttgaaggggactggaaagaacatggcatggtggaaaaaaccggaatgattatttttagcggttcccctgagggggttatggatgaatttcataacccgtatgcgtataacctgtatcgcctggatacccagggcggcaaaattattcagcgcattaccggccatgttctatcaggtattgagtttccgcatttaaataccaccattgatcagattacctacaatctgagcagcaactttgatccgtggctgaccccggacgggaacatcctctttagcagcgtgcaggcgaacgggtcacgcgcgggcggggagggccgcgtgatgatctgcgtggataactgggacggcgcgtatccgcgcccaatatatggcaactgcgatggcgaaattggcggcaccagtggtcgcagccaagcaaaaattacctttggcgaccggaaaattgtgtatgtggaaagcccgtatatgaactggggcgtgggccagctggctgccgtgagctgggatgcgccgtttaacaaaacctatgaaaaactgactggtaaagatggcggtctttatcgtagtccgtaccctctgccggatgatcgcatgctggtgagctatgcggagcgaggcgacttcggcatttattggtttaacttctccaaatgcgcggccggagataaagtgtatgacgacccgaactggaacgatcatcagccggcgccggtatacgtgaaatataaaccgcgttggattaacacctttaccgcggggaagaacttcggagtgaccgtggtgacctatcaacctttcgaccaggtgaaggtagaaggctacccccatagctggggaacgtggatttgctttgatactactctgagcgatcaacccgtgggcccatatccacatcagaaagcgaaaaacgtatctcatggcgatattaaagcggtgcggataattcagggctatcagtgtgtagagcctgatagcacccgctttcgcgtcggggcgggtgcgcatctgctggggggcgagcgcagctccagtaacagcggcacagcctttcagcagcgcggcattattgggtatcagtacgtcgagtctgatggaagtaccgtgaccagccaactaagcgatgtgccgtattatatgcaaatcctggatgataaaggaatgtctgtccagaccgcgctgacctgggcttatctgcgcccctatcatggcaggatatgtagcggatgtcattatgggtcctatcgtgggcgcgcattcaaaaacattcatgctaaagcactgtataattggtggtacgatgatcgttctcactacgatagcccgttcgcatttcgctatctgaaatttgataacgacggaaactataaaggcgtgaaacacggtgaagatgtggtggtgccgagcgatatatactatggaggtccgagcggtactacgtcgcagccggtggaaggcctgacccttgacaaacagcgaacagtagactttcgccgcgatatccaaccgattctggatgcgaagtgcgctatgtgccatgactcaaacaacccgccgaacttaggtggtgggctggaattagttagcgttgacggcattgcggcgtatagccgcgcttataacagcctgctggaaccgcaacggggaaaggatccgaatatcggaggcaaatatgtgaacccgtcagctgccattaatagcctgttagtttggagactgtatgaagcggaactgagcgcgaacgcgccgcgcgaaaagatatttccgattgaggggcgcctactccataacaaatttctgacccaggatgaacgctacgccattgtggaatggattgacctcggggcacagtgggataacattccgggcccggatttttatccgggataccttgttaaa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1952005_sequence 1 aaagaggagaaatactagatgggcaaacgcaaactgggcgtgattgcgagcgcgtttgtggcgggcgcgctggtgtgcggcagcaccctggtgaacgcggaaccggtgatgaccggcggcccggtgcagggcaaagcgctgtggaccgattatagcggcatgagcaaagaagtacagggtccagtgagccagattctgtttacccagagcccgcgcaccgcgaaaggcgatccgtatcagaactatccgcattatattccggaaggcagccgcattgtgctgtttgatctgaacaccaaagaactgaaagtgctgaccaacgattttgcgaccgccttcgacccatgcacctattgggatggcaaaaaatttgcgtttgcgggcgtgcataaaaaaggcggcggctgccaaatctgggaaatgaacattgatggcagcggcctgcgccagatgactgacctcaaaggcacctgccgcagcccgatttattatgcggcgggcagcattgaagaaggcgaaggccgaatcatttggcgcgatcgctattttgaaggggactggaaagaacatggcatggtggaaaaaaccggaatgattatttttagcggttcccctgagggggttatggatgaatttcataacccgtatgcgtataacctgtatcgcctggatacccagggcggcaaaattattcagcgcattaccggccatgttctatcaggtattgagtttccgcatttaaataccaccattgatcagattacctacaatctgagcagcaactttgatccgtggctgaccccggacgggaacatcctctttagcagcgtgcaggcgaacgggtcacgcgcgggcggggagggccgcgtgatgatctgcgtggataactgggacggcgcgtatccgcgcccaatatatggcaactgcgatggcgaaattggcggcaccagtggtcgcagccaagcaaaaattacctttggcgaccggaaaattgtgtatgtggaaagcccgtatatgaactggggcgtgggccagctggctgccgtgagctgggatgcgccgtttaacaaaacctatgaaaaactgactggtaaagatggcggtctttatcgtagtccgtaccctctgccggatgatcgcatgctggtgagctatgcggagcgaggcgacttcggcatttattggtttaacttctccaaatgcgcggccggagataaagtgtatgacgacccgaactggaacgatcatcagccggcgccggtatacgtgaaatataaaccgcgttggattaacacctttaccgcggggaagaacttcggagtgaccgtggtgacctatcaacctttcgaccaggtgaaggtagaaggctacccccatagctggggaacgtggatttgctttgatactactctgagcgatcaacccgtgggcccatatccacatcagaaagcgaaaaacgtatctcatggcgatattaaagcggtgcggataattcagggctatcagtgtgtagagcctgatagcacccgctttcgcgtcggggcgggtgcgcatctgctggggggcgagcgcagctccagtaacagcggcacagcctttcagcagcgcggcattattgggtatcagtacgtcgagtctgatggaagtaccgtgaccagccaactaagcgatgtgccgtattatatgcaaatcctggatgataaaggaatgtctgtccagaccgcgctgacctgggcttatctgcgcccctatcatggcaggatatgtagcggatgtcattatgggtcctatcgtgggcgcgcattcaaaaacattcatgctaaagcactgtataattggtggtacgatgatcgttctcactacgatagcccgttcgcatttcgctatctgaaatttgataacgacggaaactataaaggcgtgaaacacggtgaagatgtggtggtgccgagcgatatatactatggaggtccgagcggtactacgtcgcagccggtggaaggcctgacccttgacaaacagcgaacagtagactttcgccgcgatatccaaccgattctggatgcgaagtgcgctatgtgccatgactcaaacaacccgccgaacttaggtggtgggctggaattagttagcgttgacggcattgcggcgtatagccgcgcttataacagcctgctggaaccgcaacggggaaaggatccgaatatcggaggcaaatatgtgaacccgtcagctgccattaatagcctgttagtttggagactgtatgaagcggaactgagcgcgaacgcgccgcgcgaaaagatatttccgattgaggggcgcctactccataacaaatttctgacccaggatgaacgctacgccattgtggaatggattgacctcggggcacagtgggataacattccgggcccggatttttatccgggataccttgttaaa BBa_K1952008_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgggcaaacgcaaactgggcgtgattgcgagcgcgtttgtggcgggcgcgctggtgtgcggcagcaccctggtgaacgcggaaccggtgatgaccggcggcccggtgcagggcaaagcgctgtggaccgattatagcggcatgagcaaagaagtacagggtccagtgagccagattctgtttacccagagcccgcgcaccgcgaaaggcgatccgtatcagaactatccgcattatattccggaaggcagccgcattgtgctgtttgatctgaacaccaaagaactgaaagtgctgaccaacgattttgcgaccgccttcgacccatgcacctattgggatggcaaaaaatttgcgtttgcgggcgtgcataaaaaaggcggcggctgccaaatctgggaaatgaacattgatggcagcggcctgcgccagatgactgacctcaaaggcacctgccgcagcccgatttattatgcggcgggcagcattgaagaaggcgaaggccgaatcatttggcgcgatcgctattttgaaggggactggaaagaacatggcatggtggaaaaaaccggaatgattatttttagcggttcccctgagggggttatggatgaatttcataacccgtatgcgtataacctgtatcgcctggatacccagggcggcaaaattattcagcgcattaccggccatgttctatcaggtattgagtttccgcatttaaataccaccattgatcagattacctacaatctgagcagcaactttgatccgtggctgaccccggacgggaacatcctctttagcagcgtgcaggcgaacgggtcacgcgcgggcggggagggccgcgtgatgatctgcgtggataactgggacggcgcgtatccgcgcccaatatatggcaactgcgatggcgaaattggcggcaccagtggtcgcagccaagcaaaaattacctttggcgaccggaaaattgtgtatgtggaaagcccgtatatgaactggggcgtgggccagctggctgccgtgagctgggatgcgccgtttaacaaaacctatgaaaaactgactggtaaagatggcggtctttatcgtagtccgtaccctctgccggatgatcgcatgctggtgagctatgcggagcgaggcgacttcggcatttattggtttaacttctccaaatgcgcggccggagataaagtgtatgacgacccgaactggaacgatcatcagccggcgccggtatacgtgaaatataaaccgcgttggattaacacctttaccgcggggaagaacttcggagtgaccgtggtgacctatcaacctttcgaccaggtgaaggtagaaggctacccccatagctggggaacgtggatttgctttgatactactctgagcgatcaacccgtgggcccatatccacatcagaaagcgaaaaacgtatctcatggcgatattaaagcggtgcggataattcagggctatcagtgtgtagagcctgatagcacccgctttcgcgtcggggcgggtgcgcatctgctggggggcgagcgcagctccagtaacagcggcacagcctttcagcagcgcggcattattgggtatcagtacgtcgagtctgatggaagtaccgtgaccagccaactaagcgatgtgccgtattatatgcaaatcctggatgataaaggaatgtctgtccagaccgcgctgacctgggcttatctgcgcccctatcatggcaggatatgtagcggatgtcattatgggtcctatcgtgggcgcgcattcaaaaacattcatgctaaagcactgtataattggtggtacgatgatcgttctcactacgatagcccgttcgcatttcgctatctgaaatttgataacgacggaaactataaaggcgtgaaacacggtgaagatgtggtggtgccgagcgatatatactatggaggtccgagcggtactacgtcgcagccggtggaaggcctgacccttgacaaacagcgaacagtagactttcgccgcgatatccaaccgattctggatgcgaagtgcgctatgtgccatgactcaaacaacccgccgaacttaggtggtgggctggaattagttagcgttgacggcattgcggcgtatagccgcgcttataacagcctgctggaaccgcaacggggaaaggatccgaatatcggaggcaaatatgtgaacccgtcagctgccattaatagcctgttagtttggagactgtatgaagcggaactgagcgcgaacgcgccgcgcgaaaagatatttccgattgaggggcgcctactccataacaaatttctgacccaggatgaacgctacgccattgtggaatggattgacctcggggcacagtgggataacattccgggcccggatttttatccgggataccttgttaaa BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z