BBa_K1952010 1 BBa_K1952010 Hydrazine Synthase subunit gamma (Kust-2859) with LacZ reporter 2016-10-18T11:00:00Z 2016-10-29T09:51:17Z Hydrazine Synthase subunit gamma (Kust-2859) with strong RBS under lactose-inducible promoter, followed by LacZ alpha subunit reporter gene. Hydrazine Synthase subunit gamma (Kust-2859) with strong RBS under lactose-inducible promoter, followed by LacZ alpha subunit reporter gene. false false _2419_ 26856 32346 9 false none false Jessica Zhao component2523052 1 BBa_B0034 component2523053 1 BBa_K1952013 component2523050 1 BBa_K1592006 annotation2523052 1 BBa_B0034 range2523052 1 1512 1523 annotation2523053 1 BBa_K1952013 range2523053 1 1530 1871 annotation2523050 1 BBa_K1592006 range2523050 1 1 1503 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1952013 1 BBa_K1952013 LacZ alpha peptide 2016-10-18T11:00:00Z 2016-10-19T05:24:40Z Synthesized DNA Coding region of LacZ alpha, optimized for E coli and iGEM use false false _2419_ 32346 32346 9 false None false Jessica Zhao BBa_K1592006 1 AD-CIB1 GalAD-CIB1 Fusion for Yeast-Two-Hybrid 2015-09-03T11:00:00Z 2015-09-17T07:12:43Z Plasmid from Addgene. CIB1, a basic helix-loop-helix (bHLH) protein, would interact with cryptochrome 2 (CRY2), a blue light stimulated photoreceptor, when exposed to blue light. The CRY2/CIB1 interaction is entirely genetically encoded and does not require addition of any exogenous cofactors. These modules require no exogenous chromophore, are reversible within minutes, trigger protein translocation on a sub-second time scale, and even allow potential use in vivo in whole organisms. This fusion protein is for use in a yeast-two-hybrid system, and a Gal4 DNA activating domain fused to its C terminus. To regulate DNA transcription by blue light, the system is based on a two-hybrid interaction in which a light-mediated protein interaction brings together two halves (a binding domain and an activation domain) of a split transcription factor. If we remove the stimulation of blue light, dark reversion of CRY2 will dissociate the interaction with CIB1 and halt Gal4-dependent transcription. false false _2009_ 20267 20267 9 true No false Shuyan Tang annotation2443465 1 Gal Activating Domain range2443465 1 1 408 annotation2443466 1 CIB1 range2443466 1 457 1460 BBa_B0034_sequence 1 aaagaggagaaa BBa_K1952010_sequence 1 atggataaagcggaattaattcccgagcctccaaaaaagaagagaaaggtcgaattgggtaccgccgccaattttaatcaaagtgggaatattgctgatagctcattgtccttcactttcactaacagtagcaacggtccgaacctcataacaactcaaacaaattctcaagcgctttcacaaccaattgcctcctctaacgttcatgataacttcatgaataatgaaatcacggctagtaaaattgatgatggtaataattcaaaaccactgtcacctggttggacggaccaaactgcgtataacgcgtttggaatcactacagggatgtttaataccactacaatggatgatgtatataactatctattcgatgatgaagataccccaccaaacccaaaaaaagagatctttaatacgactcactatagggcgagcgccgaagctagcgccaccatgaatggagctataggaggtgaccttttgctcaattttcctgacatgtcggtcctagagcgccaaagggctcacctcaagtacctcaatcccacctttgattctcctctcgccggcttctttgccgattcttcaatgattaccggcggcgagatggacagctatctttcgactgccggtttgaatcttccgatgatgtacggtgagacgacggtggaaggtgattcaagactctcaatttcgccggaaacgacgcttgggactggaaatttcaagaaacggaagtttgatacagagactaaggattgtaatgagaagaagaagaagatgacgatgaacagagatgacctagtagaagaaggagaagaagagaagtcgaaaataacagagcaaaacaatgggagcacaaaaagcatcaagaagatgaaacacaaagccaagaaagaagagaacaatttctctaatgattcatctaaagtgacgaaggaattggagaaaacggattatattcatgttcgtgcacgacgaggccaagccactgatagtcacagcatagcagaacgagttagaagagaaaagatcagtgagagaatgaagtttctacaagatttggttcctggatgcgacaagatcacaggcaaagcagggatgcttgatgaaatcattaactatgttcagtctcttcagagacaaatcgagttcttatcgatgaaactagcaattgtgaatccaaggccggattttgatatggatgacatttttgccaaagaggttgcctcaactccaatgactgtggtgccatctcctgaaatggttctttccggttattctcatgagatggttcactctggttattctagtgagatggttaactccggttaccttcatgtcaatccaatgcagcaagtgaataccagttctgatccattgtcatgcttcaacaatggcgaagctccttcgatgtgggactctcatgtgcagaatctctatggcaatttaggagtaccggtcatcgagctcgagctccagatgaatcgtagatactgatactagagaaagaggagaaatactagatgaccatgattaccccgagcctgcatgcgtgccgcagcaccctggaagatccgcgcgtccccagcagcaacagcctggcggtggtgttacaacgccgcgattgggaaaacccgggcgtgacccagctgaaccgcctcgccgcgcatccgccgtttgcgagctggcgcaattccgaagaagcgcgcaccgatcgccctagtcagcagctgcgcagcctgaacggcgaatggcgcctgatgcgctattttctgctgacccatctgtgcggcattagccatcgcatttggtgcactcttagcaccatttgcagcgatgcggcgcatcaccaccatcatcattag BBa_K1952013_sequence 1 atgaccatgattaccccgagcctgcatgcgtgccgcagcaccctggaagatccgcgcgtccccagcagcaacagcctggcggtggtgttacaacgccgcgattgggaaaacccgggcgtgacccagctgaaccgcctcgccgcgcatccgccgtttgcgagctggcgcaattccgaagaagcgcgcaccgatcgccctagtcagcagctgcgcagcctgaacggcgaatggcgcctgatgcgctattttctgctgacccatctgtgcggcattagccatcgcatttggtgcactcttagcaccatttgcagcgatgcggcgcatcaccaccatcatcattag BBa_K1592006_sequence 1 atggataaagcggaattaattcccgagcctccaaaaaagaagagaaaggtcgaattgggtaccgccgccaattttaatcaaagtgggaatattgctgatagctcattgtccttcactttcactaacagtagcaacggtccgaacctcataacaactcaaacaaattctcaagcgctttcacaaccaattgcctcctctaacgttcatgataacttcatgaataatgaaatcacggctagtaaaattgatgatggtaataattcaaaaccactgtcacctggttggacggaccaaactgcgtataacgcgtttggaatcactacagggatgtttaataccactacaatggatgatgtatataactatctattcgatgatgaagataccccaccaaacccaaaaaaagagatctttaatacgactcactatagggcgagcgccgaagctagcgccaccatgaatggagctataggaggtgaccttttgctcaattttcctgacatgtcggtcctagagcgccaaagggctcacctcaagtacctcaatcccacctttgattctcctctcgccggcttctttgccgattcttcaatgattaccggcggcgagatggacagctatctttcgactgccggtttgaatcttccgatgatgtacggtgagacgacggtggaaggtgattcaagactctcaatttcgccggaaacgacgcttgggactggaaatttcaagaaacggaagtttgatacagagactaaggattgtaatgagaagaagaagaagatgacgatgaacagagatgacctagtagaagaaggagaagaagagaagtcgaaaataacagagcaaaacaatgggagcacaaaaagcatcaagaagatgaaacacaaagccaagaaagaagagaacaatttctctaatgattcatctaaagtgacgaaggaattggagaaaacggattatattcatgttcgtgcacgacgaggccaagccactgatagtcacagcatagcagaacgagttagaagagaaaagatcagtgagagaatgaagtttctacaagatttggttcctggatgcgacaagatcacaggcaaagcagggatgcttgatgaaatcattaactatgttcagtctcttcagagacaaatcgagttcttatcgatgaaactagcaattgtgaatccaaggccggattttgatatggatgacatttttgccaaagaggttgcctcaactccaatgactgtggtgccatctcctgaaatggttctttccggttattctcatgagatggttcactctggttattctagtgagatggttaactccggttaccttcatgtcaatccaatgcagcaagtgaataccagttctgatccattgtcatgcttcaacaatggcgaagctccttcgatgtgggactctcatgtgcagaatctctatggcaatttaggagtaccggtcatcgagctcgagctccagatgaatcgtagatactga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z