BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation1999 1 lac O1 range1999 1 3 19 annotation7064 1 BBa_R0011 range7064 1 1 54 annotation2001 1 lac O1 range2001 1 26 42 annotation2002 1 -10 range2002 1 43 48 annotation2000 1 -35 range2000 1 20 25 BBa_K1952000 1 BBa_K1952000 Hydrazine Synthase gamma subunit 2016-10-13T11:00:00Z 2016-10-29T09:10:52Z source hydrazine synthase subunit 1 false false _2419_ 26856 33370 9 false design false Alex Dean BBa_K1952012 1 BBa_K1952012 Hydrazine Synthase subunit alpha (Kust-2861) with LacZ reporter 2016-10-18T11:00:00Z 2016-10-29T09:52:21Z Hydrazine Synthase subunit alpha (Kust-2861) with strong RBS under lactose-inducible promoter, followed by LacZ alpha subunit reporter gene. Hydrazine Synthase subunit alpha (Kust-2861) with strong RBS under lactose-inducible promoter, followed by LacZ alpha subunit reporter gene. false false _2419_ 26856 32346 9 It's complicated false none false Jessica Zhao component2523103 1 BBa_B0034 component2523101 1 BBa_K1952006 component2523104 1 BBa_K1952013 annotation2523101 1 BBa_K1952006 range2523101 1 1 1239 annotation2523104 1 BBa_K1952013 range2523104 1 1266 1607 annotation2523103 1 BBa_B0034 range2523103 1 1248 1259 BBa_K1952013 1 BBa_K1952013 LacZ alpha peptide 2016-10-18T11:00:00Z 2016-10-19T05:24:40Z Synthesized DNA Coding region of LacZ alpha, optimized for E coli and iGEM use false false _2419_ 32346 32346 9 false None false Jessica Zhao BBa_K1952006 1 BBa_K1952006 Hydrazine Synthase subunit gamma (Kust-2859) with strong RBS under lactose-inducible promoter 2016-10-18T11:00:00Z 2016-10-19T04:52:58Z Hydrazine Synthase subunit gamma (Kust-2859) with strong RBS under lactose-inducible promoter Protein coding region with translation initiation signals, to be used alone or in operon false false _2419_ 32346 32346 9 false none false Jessica Zhao component2522955 1 BBa_K1952003 component2522947 1 BBa_R0011 annotation2522955 1 BBa_K1952003 range2522955 1 64 1239 annotation2522947 1 BBa_R0011 range2522947 1 1 54 BBa_K1952003 1 BBa_K1952003 Hydrazine Synthase subunit gamma (Kust-2859) with strong RBS 2016-10-18T11:00:00Z 2016-10-19T04:43:21Z B0034 RBS and synthesized DNA of Hydrazine Synthase (part K1952000) Protein coding region with translation initiation signals. Can be used alone with promoter or combined into a larger operon. false false _2419_ 32346 32346 9 false None false Jessica Zhao component2522881 1 BBa_K1952000 component2522880 1 BBa_B0034 annotation2522881 1 BBa_K1952000 range2522881 1 19 1176 annotation2522880 1 BBa_B0034 range2522880 1 1 12 BBa_K1952012_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggtgattcgccgcaaaatgaacaagatgatcaggaaaggcatgattggcgcggtgatgctgggcgcggcggtggcgattagcggcggcgtggcgaccgcgggctatattcagggcacccatgtgaaaaccgatctgccgggcccgtttcatattaccatgagcccggatggcagcaccctgtttattagcaaccagagcggccatagcgtgacctttgtggatgcgcgcacccagaaagtgaccggcgaagtggcggtgcgcgtgcagccggaagcgagcgccgtcaccccagatggcgcgtttctgtatgtgtgcaacgcggaaagcgatagtgttagcgtggtggatatacaacgcaaacaggaaattaaagagataaaagtgggcgattggccgagcggcattaaaatttcgcccgatggcaaaaccgcgtatgtggcttgtagcggctgcatgtggaacgcgattgatgtgattgataccggccgcatggaaaaagtgcgcagcatttataccagcgattatggcccgcgcatggtggagataagccctgacggaaagaccctggtagcaattctggataccgtgggctcaatcaaccgcagcgtggattttattgatattgcgtccggtcgcgtcgtagagaatcgcgtgattcatgaaagcagcaacctgcgcgatgtggtgtataccccggatggcaaatatatagccgtgacccatcagaccccgaaaaactggctgccggtgtgcgaagcggaaaacggccaggtgtttaccaacaacgtgaccattattgaaaccaaagcgggcggcaaagtagcacgcctgccgctggatgatctgaacaactatgacgggaacccgtatggcatggcgatggatccgaagggaaaatatctgtatattggcgtgcgcggcatgcatcgcgtaacaatcctagatatggataaagtgcttgggctggtacggagctcaactcaggaagaactggattatttaagggacgatctgggcctggtaagggattatctggtggcgagagttccgaccggcctgggcccgagctcagtctgcctgtccccagatggtaaattttgctatgcggcgaactatttttcgaataatgtaaccgtcatacgcaccgcggtggattactagagaaagaggagaaatactagatgaccatgattaccccgagcctgcatgcgtgccgcagcaccctggaagatccgcgcgtccccagcagcaacagcctggcggtggtgttacaacgccgcgattgggaaaacccgggcgtgacccagctgaaccgcctcgccgcgcatccgccgtttgcgagctggcgcaattccgaagaagcgcgcaccgatcgccctagtcagcagctgcgcagcctgaacggcgaatggcgcctgatgcgctattttctgctgacccatctgtgcggcattagccatcgcatttggtgcactcttagcaccatttgcagcgatgcggcgcatcaccaccatcatcattag BBa_B0034_sequence 1 aaagaggagaaa BBa_K1952000_sequence 1 atggtgattcgccgcaaaatgaacaagatgatcaggaaaggcatgattggcgcggtgatgctgggcgcggcggtggcgattagcggcggcgtggcgaccgcgggctatattcagggcacccatgtgaaaaccgatctgccgggcccgtttcatattaccatgagcccggatggcagcaccctgtttattagcaaccagagcggccatagcgtgacctttgtggatgcgcgcacccagaaagtgaccggcgaagtggcggtgcgcgtgcagccggaagcgagcgccgtcaccccagatggcgcgtttctgtatgtgtgcaacgcggaaagcgatagtgttagcgtggtggatatacaacgcaaacaggaaattaaagagataaaagtgggcgattggccgagcggcattaaaatttcgcccgatggcaaaaccgcgtatgtggcttgtagcggctgcatgtggaacgcgattgatgtgattgataccggccgcatggaaaaagtgcgcagcatttataccagcgattatggcccgcgcatggtggagataagccctgacggaaagaccctggtagcaattctggataccgtgggctcaatcaaccgcagcgtggattttattgatattgcgtccggtcgcgtcgtagagaatcgcgtgattcatgaaagcagcaacctgcgcgatgtggtgtataccccggatggcaaatatatagccgtgacccatcagaccccgaaaaactggctgccggtgtgcgaagcggaaaacggccaggtgtttaccaacaacgtgaccattattgaaaccaaagcgggcggcaaagtagcacgcctgccgctggatgatctgaacaactatgacgggaacccgtatggcatggcgatggatccgaagggaaaatatctgtatattggcgtgcgcggcatgcatcgcgtaacaatcctagatatggataaagtgcttgggctggtacggagctcaactcaggaagaactggattatttaagggacgatctgggcctggtaagggattatctggtggcgagagttccgaccggcctgggcccgagctcagtctgcctgtccccagatggtaaattttgctatgcggcgaactatttttcgaataatgtaaccgtcatacgcaccgcggtggat BBa_K1952013_sequence 1 atgaccatgattaccccgagcctgcatgcgtgccgcagcaccctggaagatccgcgcgtccccagcagcaacagcctggcggtggtgttacaacgccgcgattgggaaaacccgggcgtgacccagctgaaccgcctcgccgcgcatccgccgtttgcgagctggcgcaattccgaagaagcgcgcaccgatcgccctagtcagcagctgcgcagcctgaacggcgaatggcgcctgatgcgctattttctgctgacccatctgtgcggcattagccatcgcatttggtgcactcttagcaccatttgcagcgatgcggcgcatcaccaccatcatcattag BBa_K1952006_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggtgattcgccgcaaaatgaacaagatgatcaggaaaggcatgattggcgcggtgatgctgggcgcggcggtggcgattagcggcggcgtggcgaccgcgggctatattcagggcacccatgtgaaaaccgatctgccgggcccgtttcatattaccatgagcccggatggcagcaccctgtttattagcaaccagagcggccatagcgtgacctttgtggatgcgcgcacccagaaagtgaccggcgaagtggcggtgcgcgtgcagccggaagcgagcgccgtcaccccagatggcgcgtttctgtatgtgtgcaacgcggaaagcgatagtgttagcgtggtggatatacaacgcaaacaggaaattaaagagataaaagtgggcgattggccgagcggcattaaaatttcgcccgatggcaaaaccgcgtatgtggcttgtagcggctgcatgtggaacgcgattgatgtgattgataccggccgcatggaaaaagtgcgcagcatttataccagcgattatggcccgcgcatggtggagataagccctgacggaaagaccctggtagcaattctggataccgtgggctcaatcaaccgcagcgtggattttattgatattgcgtccggtcgcgtcgtagagaatcgcgtgattcatgaaagcagcaacctgcgcgatgtggtgtataccccggatggcaaatatatagccgtgacccatcagaccccgaaaaactggctgccggtgtgcgaagcggaaaacggccaggtgtttaccaacaacgtgaccattattgaaaccaaagcgggcggcaaagtagcacgcctgccgctggatgatctgaacaactatgacgggaacccgtatggcatggcgatggatccgaagggaaaatatctgtatattggcgtgcgcggcatgcatcgcgtaacaatcctagatatggataaagtgcttgggctggtacggagctcaactcaggaagaactggattatttaagggacgatctgggcctggtaagggattatctggtggcgagagttccgaccggcctgggcccgagctcagtctgcctgtccccagatggtaaattttgctatgcggcgaactatttttcgaataatgtaaccgtcatacgcaccgcggtggat BBa_K1952003_sequence 1 aaagaggagaaatactagatggtgattcgccgcaaaatgaacaagatgatcaggaaaggcatgattggcgcggtgatgctgggcgcggcggtggcgattagcggcggcgtggcgaccgcgggctatattcagggcacccatgtgaaaaccgatctgccgggcccgtttcatattaccatgagcccggatggcagcaccctgtttattagcaaccagagcggccatagcgtgacctttgtggatgcgcgcacccagaaagtgaccggcgaagtggcggtgcgcgtgcagccggaagcgagcgccgtcaccccagatggcgcgtttctgtatgtgtgcaacgcggaaagcgatagtgttagcgtggtggatatacaacgcaaacaggaaattaaagagataaaagtgggcgattggccgagcggcattaaaatttcgcccgatggcaaaaccgcgtatgtggcttgtagcggctgcatgtggaacgcgattgatgtgattgataccggccgcatggaaaaagtgcgcagcatttataccagcgattatggcccgcgcatggtggagataagccctgacggaaagaccctggtagcaattctggataccgtgggctcaatcaaccgcagcgtggattttattgatattgcgtccggtcgcgtcgtagagaatcgcgtgattcatgaaagcagcaacctgcgcgatgtggtgtataccccggatggcaaatatatagccgtgacccatcagaccccgaaaaactggctgccggtgtgcgaagcggaaaacggccaggtgtttaccaacaacgtgaccattattgaaaccaaagcgggcggcaaagtagcacgcctgccgctggatgatctgaacaactatgacgggaacccgtatggcatggcgatggatccgaagggaaaatatctgtatattggcgtgcgcggcatgcatcgcgtaacaatcctagatatggataaagtgcttgggctggtacggagctcaactcaggaagaactggattatttaagggacgatctgggcctggtaagggattatctggtggcgagagttccgaccggcctgggcccgagctcagtctgcctgtccccagatggtaaattttgctatgcggcgaactatttttcgaataatgtaaccgtcatacgcaccgcggtggat BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z