LOCUS       BBa_K195202             3646 bp    DNA     linear       25-Nov-2024
DEFINITION  Lpp-OmpA-linker+alpha-V integrin gene+terminator
ACCESSION   BBa_K195202
VERSION     BBa_K195202.1  
FEATURES             Location/Qualifiers
     misc_feature    1..7
                     /label=NdeI
     CDS             4..438
                     /label=OmpA
     CDS             439..464
                     /label=linker
     misc_feature    459..464
                     /label=SacI
     misc_feature    4..6
                     /label=ATG
     misc_feature    1..464
                     /label=BBa_K103006
     CDS             471..3509
                     /label=BBa_K195200
     stem_loop       3529..3572
                     /label=stem_loop
     misc_feature    3518..3597
                     /label=BBa_B0010
     terminator      3518..3597
                     /label=BBa_B0010
     stem_loop       3613..3632
                     /label=T7 TE
     misc_feature    3639..3639
                     /label=stop
     polyA_site      3633..3646
                     /label=polya
     misc_feature    3606..3646
                     /label=BBa_B0012
     terminator      3606..3646
                     /label=BBa_B0012
ORIGIN
        1 catatgaaag ctactaaact ggtactgggc gcggtaatcc tgggttctac tctgctggca
       61 ggttgctcca gcaacgctaa aatcgatcag ggaattaacc cgtatgttgg ctttgaaatg
      121 ggttacgact ggttaggtcg tatgccgtac aaaggcagcg ttgaaaacgg tgcatacaaa
      181 gctcagggcg ttcaactgac cgctaaactg ggttacccaa tcactgacga cctggacatc
      241 tacactcgtc tgggtggcat ggtatggcgt gcagacacta aatccaacgt ttatggtaaa
      301 aaccacgaca ccggcgtttc tccggtcttc gctggcggtg ttgagtacgc gatcactcct
      361 gaaatcgcta cccgtctgga ataccagtgg accaacaaca tcggtgacgc acacaccatc
      421 ggcactcgtc cggacaacgg cggaggttct ggaggaggga gctctactag atggcttttc
      481 cgccgcggcg acggctgcgc ctcggtcccc gcggcctccc gcttcttctc tcgggactcc
      541 tgctacctct gtgccgcgcc ttcaacctag acgtggacag tcctgccgag tactctggcc
      601 ccgagggaag ttacttcggc ttcgccgtgg atttcttcgt gcccagcgcg tcttcccgga
      661 tgtttcttct cgtgggagct cccaaagcaa acaccaccca gcctgggatt gtggaaggag
      721 ggcaggtcct caaatgtgac tggtcttcta cccgccggtg ccagccaatt gaatttgatg
      781 caacaggcaa tagagattat gccaaggatg atccattgga atttaagtcc catcagtggt
      841 ttggagcatc tgtgaggtcg aaacaggata aaattttggc ctgtgcccca ttgtaccatt
      901 ggagaactga gatgaaacag gagcgagagc ctgttggaac atgctttctt caagatggaa
      961 caaagactgt tgagtatgct ccatgtagat cacgtcagct tatttcggat caagtggcag
     1021 aaatcgtatc taaatacgac cccaatgttt acagcatcaa gtataataac caattagcaa
     1081 ctcggactgc acaagctatt tttgatgaca gctatttggg ttattctgtg gctgtcggag
     1141 atttcaatgg tgatggcata gatgactttg tttcaggagt tccaagagca gcaaggactt
     1201 tgggaatggt ttatatttat gatgggaaga acatgtcctc cttatacaat tttactggcg
     1261 agcagatggc tgcatatttc ggattttctg tagctgccac tgacattaat ggagatgatt
     1321 atgcagatgt gtttattgga gcacctctct tcatggatcg tggctctgat ggcaaactcc
     1381 aagaggtggg gcaggtctca gtgtctctac agagagcttc aggagacttc cagacgacaa
     1441 agctgaatgg atttgaggtc tttgcacggt ttggcagtgc catagctcct ttgggagatc
     1501 tggaccagga tggtttcaat gatattgcaa ttgctgctcc atatgggggt gaagataaaa
     1561 aaggaattgt ttatatcttc aatggaagat caacaggctt gaacgcagtc ccatctcaaa
     1621 tccttgaagg gcagtgggct gctcgaagca tgccaccaag ctttggctat tcaatgaaag
     1681 gagccacaga tatagacaaa aatggatatc cagacttaat tgtaggagct tttggtgtag
     1741 atcgagctat cttatacagg gccagaccag ttatcactgt aaatgctggt cttgaagtgt
     1801 accctagcat tttaaatcaa gacaataaaa cctgctcact gcctggaaca gctctcaaag
     1861 tttcctgttt taatgttagg ttctgcttaa aggcagatgg caaaggagta cttcccagga
     1921 aacttaattt ccaggtggaa cttcttttgg ataaactcaa gcaaaaggga gcaattcgac
     1981 gagcactgtt tctctacagc aggtccccaa gtcactccaa gaacatgact atttcaaggg
     2041 ggggactgat gcagtgtgag gaattgatag cgtatctgcg ggatgaatct gaatttagag
     2101 acaaactcac tccaattact atttttatgg aatatcggtt ggattataga acagctgctg
     2161 atacaacagg cttgcaaccc attcttaacc agttcacgcc tgctaacatt agtcgacagg
     2221 ctcacattct acttgactgt ggtgaagaca atgtctgtaa acccaagctg gaagtttctg
     2281 tagatagtga tcaaaagaag atctatattg gggatgacaa ccctctgaca ttgattgtta
     2341 aggctcagaa tcaaggagaa ggtgcctacg aagctgagct catcgtttcc attccactgc
     2401 aggctgattt catcggggtt gtccgaaaca atgaagcctt agcaagactt tcctgtgcat
     2461 ttaagacaga aaaccaaact cgccaggtgg tatgtgacct tggaaaccca atgaaggctg
     2521 gaactcaact cttagctggt cttcgtttca gtgtgcacca gcagtcagag atggatactt
     2581 ctgtgaaatt tgacttacaa atccaaagct caaatctatt tgacaaagta agcccagttg
     2641 tatctcacaa agttgatctt gctgttttag ctgcagttga gataagagga gtctcgagtc
     2701 ctgatcatat ctttcttccg attccaaact gggagcacaa ggagaaccct gagactgaag
     2761 aagatgttgg gccagttgtt cagcacatct atgagctgag aaacaatggt ccaagttcat
     2821 tcagcaaggc aatgctccat cttcagtggc cttacaaata taataataac actctgttgt
     2881 atatccttca ttatgatatt gatggaccaa tgaactgcac ttcagatatg gagatcaacc
     2941 ctttgagaat taagatctca tctttgcaaa caactgaaaa gaatgacacg gttgccgggc
     3001 aaggtgagcg ggaccatctc atcactaagc gggatcttgc cctcagtgaa ggagatattc
     3061 acactttggg ttgtggagtt gctcagtgct tgaagattgt ctgccaagtt gggagattag
     3121 acagaggaaa gagtgcaatc ttgtacgtaa agtcattact gtggactgag acttttatga
     3181 ataaagaaaa tcagaatcat tcctattctc tgaagtcgtc tgcttcattt aatgtcatag
     3241 agtttcctta taagaatctt ccaattgagg atatcaccaa ctccacattg gttaccacta
     3301 atgtcacctg gggcattcag ccagcgccca tgcctgtgcc tgtgtgggtg atcattttag
     3361 cagttctagc aggattgttg ctactggctg ttttggtatt tgtaatgtac aggatgggct
     3421 tttttaaacg ggtccggcca cctcaagaag aacaagaaag ggagcagctt caacctcatg
     3481 aaaatggtga aggaaactca gaaacttaat actagagcca ggcatcaaat aaaacgaaag
     3541 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     3601 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//