LOCUS BBa_K195204 3728 bp DNA linear 26-Nov-2024 DEFINITION pTet+RBS+Lpp-OmpA-linker+alpha-V integrin gene+terminator ACCESSION BBa_K195204 VERSION BBa_K195204.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 83..89 /label=NdeI CDS 86..520 /label=OmpA CDS 521..546 /label=linker misc_feature 541..546 /label=SacI misc_feature 86..88 /label=ATG misc_feature 83..546 /label=BBa_K103006 CDS 553..3591 /label=BBa_K195200 stem_loop 3611..3654 /label=stem_loop misc_feature 3600..3679 /label=BBa_B0010 terminator 3600..3679 /label=BBa_B0010 stem_loop 3695..3714 /label=T7 TE misc_feature 3721..3721 /label=stop polyA_site 3715..3728 /label=polya misc_feature 3688..3728 /label=BBa_B0012 terminator 3688..3728 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag agcatatgaa agctactaaa ctggtactgg gcgcggtaat 121 cctgggttct actctgctgg caggttgctc cagcaacgct aaaatcgatc agggaattaa 181 cccgtatgtt ggctttgaaa tgggttacga ctggttaggt cgtatgccgt acaaaggcag 241 cgttgaaaac ggtgcataca aagctcaggg cgttcaactg accgctaaac tgggttaccc 301 aatcactgac gacctggaca tctacactcg tctgggtggc atggtatggc gtgcagacac 361 taaatccaac gtttatggta aaaaccacga caccggcgtt tctccggtct tcgctggcgg 421 tgttgagtac gcgatcactc ctgaaatcgc tacccgtctg gaataccagt ggaccaacaa 481 catcggtgac gcacacacca tcggcactcg tccggacaac ggcggaggtt ctggaggagg 541 gagctctact agatggcttt tccgccgcgg cgacggctgc gcctcggtcc ccgcggcctc 601 ccgcttcttc tctcgggact cctgctacct ctgtgccgcg ccttcaacct agacgtggac 661 agtcctgccg agtactctgg ccccgaggga agttacttcg gcttcgccgt ggatttcttc 721 gtgcccagcg cgtcttcccg gatgtttctt ctcgtgggag ctcccaaagc aaacaccacc 781 cagcctggga ttgtggaagg agggcaggtc ctcaaatgtg actggtcttc tacccgccgg 841 tgccagccaa ttgaatttga tgcaacaggc aatagagatt atgccaagga tgatccattg 901 gaatttaagt cccatcagtg gtttggagca tctgtgaggt cgaaacagga taaaattttg 961 gcctgtgccc cattgtacca ttggagaact gagatgaaac aggagcgaga gcctgttgga 1021 acatgctttc ttcaagatgg aacaaagact gttgagtatg ctccatgtag atcacgtcag 1081 cttatttcgg atcaagtggc agaaatcgta tctaaatacg accccaatgt ttacagcatc 1141 aagtataata accaattagc aactcggact gcacaagcta tttttgatga cagctatttg 1201 ggttattctg tggctgtcgg agatttcaat ggtgatggca tagatgactt tgtttcagga 1261 gttccaagag cagcaaggac tttgggaatg gtttatattt atgatgggaa gaacatgtcc 1321 tccttataca attttactgg cgagcagatg gctgcatatt tcggattttc tgtagctgcc 1381 actgacatta atggagatga ttatgcagat gtgtttattg gagcacctct cttcatggat 1441 cgtggctctg atggcaaact ccaagaggtg gggcaggtct cagtgtctct acagagagct 1501 tcaggagact tccagacgac aaagctgaat ggatttgagg tctttgcacg gtttggcagt 1561 gccatagctc ctttgggaga tctggaccag gatggtttca atgatattgc aattgctgct 1621 ccatatgggg gtgaagataa aaaaggaatt gtttatatct tcaatggaag atcaacaggc 1681 ttgaacgcag tcccatctca aatccttgaa gggcagtggg ctgctcgaag catgccacca 1741 agctttggct attcaatgaa aggagccaca gatatagaca aaaatggata tccagactta 1801 attgtaggag cttttggtgt agatcgagct atcttataca gggccagacc agttatcact 1861 gtaaatgctg gtcttgaagt gtaccctagc attttaaatc aagacaataa aacctgctca 1921 ctgcctggaa cagctctcaa agtttcctgt tttaatgtta ggttctgctt aaaggcagat 1981 ggcaaaggag tacttcccag gaaacttaat ttccaggtgg aacttctttt ggataaactc 2041 aagcaaaagg gagcaattcg acgagcactg tttctctaca gcaggtcccc aagtcactcc 2101 aagaacatga ctatttcaag ggggggactg atgcagtgtg aggaattgat agcgtatctg 2161 cgggatgaat ctgaatttag agacaaactc actccaatta ctatttttat ggaatatcgg 2221 ttggattata gaacagctgc tgatacaaca ggcttgcaac ccattcttaa ccagttcacg 2281 cctgctaaca ttagtcgaca ggctcacatt ctacttgact gtggtgaaga caatgtctgt 2341 aaacccaagc tggaagtttc tgtagatagt gatcaaaaga agatctatat tggggatgac 2401 aaccctctga cattgattgt taaggctcag aatcaaggag aaggtgccta cgaagctgag 2461 ctcatcgttt ccattccact gcaggctgat ttcatcgggg ttgtccgaaa caatgaagcc 2521 ttagcaagac tttcctgtgc atttaagaca gaaaaccaaa ctcgccaggt ggtatgtgac 2581 cttggaaacc caatgaaggc tggaactcaa ctcttagctg gtcttcgttt cagtgtgcac 2641 cagcagtcag agatggatac ttctgtgaaa tttgacttac aaatccaaag ctcaaatcta 2701 tttgacaaag taagcccagt tgtatctcac aaagttgatc ttgctgtttt agctgcagtt 2761 gagataagag gagtctcgag tcctgatcat atctttcttc cgattccaaa ctgggagcac 2821 aaggagaacc ctgagactga agaagatgtt gggccagttg ttcagcacat ctatgagctg 2881 agaaacaatg gtccaagttc attcagcaag gcaatgctcc atcttcagtg gccttacaaa 2941 tataataata acactctgtt gtatatcctt cattatgata ttgatggacc aatgaactgc 3001 acttcagata tggagatcaa ccctttgaga attaagatct catctttgca aacaactgaa 3061 aagaatgaca cggttgccgg gcaaggtgag cgggaccatc tcatcactaa gcgggatctt 3121 gccctcagtg aaggagatat tcacactttg ggttgtggag ttgctcagtg cttgaagatt 3181 gtctgccaag ttgggagatt agacagagga aagagtgcaa tcttgtacgt aaagtcatta 3241 ctgtggactg agacttttat gaataaagaa aatcagaatc attcctattc tctgaagtcg 3301 tctgcttcat ttaatgtcat agagtttcct tataagaatc ttccaattga ggatatcacc 3361 aactccacat tggttaccac taatgtcacc tggggcattc agccagcgcc catgcctgtg 3421 cctgtgtggg tgatcatttt agcagttcta gcaggattgt tgctactggc tgttttggta 3481 tttgtaatgt acaggatggg cttttttaaa cgggtccggc cacctcaaga agaacaagaa 3541 agggagcagc ttcaacctca tgaaaatggt gaaggaaact cagaaactta atactagagc 3601 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 3661 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 3721 cgtttata //