LOCUS BBa_K195209 1787 bp DNA linear 22-Dec-2024 DEFINITION pTet+RBS+Lpp-OmpA-linker+CXADR gene+terminator ACCESSION BBa_K195209 VERSION BBa_K195209.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 83..89 /label=NdeI CDS 86..520 /label=OmpA CDS 521..546 /label=linker misc_feature 541..546 /label=SacI misc_feature 86..88 /label=ATG misc_feature 83..546 /label=BBa_K103006 CDS 553..1650 /label=BBa_K195205 stem_loop 1670..1713 /label=stem_loop misc_feature 1659..1738 /label=BBa_B0010 terminator 1659..1738 /label=BBa_B0010 stem_loop 1754..1773 /label=T7 TE misc_feature 1780..1780 /label=stop polyA_site 1774..1787 /label=polya misc_feature 1747..1787 /label=BBa_B0012 terminator 1747..1787 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag agcatatgaa agctactaaa ctggtactgg gcgcggtaat 121 cctgggttct actctgctgg caggttgctc cagcaacgct aaaatcgatc agggaattaa 181 cccgtatgtt ggctttgaaa tgggttacga ctggttaggt cgtatgccgt acaaaggcag 241 cgttgaaaac ggtgcataca aagctcaggg cgttcaactg accgctaaac tgggttaccc 301 aatcactgac gacctggaca tctacactcg tctgggtggc atggtatggc gtgcagacac 361 taaatccaac gtttatggta aaaaccacga caccggcgtt tctccggtct tcgctggcgg 421 tgttgagtac gcgatcactc ctgaaatcgc tacccgtctg gaataccagt ggaccaacaa 481 catcggtgac gcacacacca tcggcactcg tccggacaac ggcggaggtt ctggaggagg 541 gagctctact agatggcgct cctgctgtgc ttcgtgctcc tgtgcggagt agtggatttc 601 gccagaagtt tgagtatcac tactcctgaa gagatgattg aaaaagccaa aggggaaact 661 gcctatctgc cgtgcaaatt tacgcttagt cccgaagacc agggaccgct ggacatcgag 721 tggctgatat caccagctga taatcagaag gtggatcaag tgattatttt atattctgga 781 gacaaaattt atgatgacta ctatccagat ctgaaaggcc gagtacattt tacgagtaat 841 gatctcaaat ctggtgatgc atcaataaat gtaacgaatt tacaactgtc agatattggc 901 acatatcagt gcaaagtgaa aaaagctcct ggtgttgcaa ataagaagat tcatctggta 961 gttcttgtta agccttcagg tgcgagatgt tacgttgatg gatctgaaga aattggaagt 1021 gactttaaga taaaatgtga accaaaagaa ggttcacttc cattacagta tgagtggcaa 1081 aaattgtctg actcacagaa aatgcccact tcatggttag cagaaatgac ttcatctgtt 1141 atatctgtaa aaaatgcctc ttctgagtac tctgggacat acagctgtac agtcagaaac 1201 agagtgggct ctgatcagtg cctgttgcgt ctaaacgttg tccctccttc aaataaagct 1261 ggactaattg caggagccat tataggaact ttgcttgctc tagcgctcat tggtcttatc 1321 atcttttgct gtcgtaaaaa gcgcagagaa gaaaaatatg aaaaggaagt tcatcacgat 1381 atcagggaag atgtgccacc tccaaagagc cgtacgtcca ctgccagaag ctacatcggc 1441 agtaatcatt catccctggg gtccatgtct ccttccaaca tggaaggata ttccaagact 1501 cagtataacc aagtaccaag tgaagacttt gaacgcactc ctcagagtcc gactctccca 1561 cctgctaagg tagctgcccc taatctaagt cgaatgggtg cgattcctgt gatgattcca 1621 gcacagagca aggatgggtc tatagtatag tactagagcc aggcatcaaa taaaacgaaa 1681 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 1741 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //