LOCUS       BBa_K195209             1787 bp    DNA     linear       17-May-2024
DEFINITION  pTet+RBS+Lpp-OmpA-linker+CXADR gene+terminator
ACCESSION   BBa_K195209
VERSION     BBa_K195209.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    83..89
                     /label=NdeI
     CDS             86..520
                     /label=OmpA
     CDS             521..546
                     /label=linker
     misc_feature    541..546
                     /label=SacI
     misc_feature    86..88
                     /label=ATG
     misc_feature    83..546
                     /label=BBa_K103006
     CDS             553..1650
                     /label=BBa_K195205
     stem_loop       1670..1713
                     /label=stem_loop
     misc_feature    1659..1738
                     /label=BBa_B0010
     terminator      1659..1738
                     /label=BBa_B0010
     stem_loop       1754..1773
                     /label=T7 TE
     misc_feature    1780..1780
                     /label=stop
     polyA_site      1774..1787
                     /label=polya
     misc_feature    1747..1787
                     /label=BBa_B0012
     terminator      1747..1787
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag agcatatgaa agctactaaa ctggtactgg gcgcggtaat
      121 cctgggttct actctgctgg caggttgctc cagcaacgct aaaatcgatc agggaattaa
      181 cccgtatgtt ggctttgaaa tgggttacga ctggttaggt cgtatgccgt acaaaggcag
      241 cgttgaaaac ggtgcataca aagctcaggg cgttcaactg accgctaaac tgggttaccc
      301 aatcactgac gacctggaca tctacactcg tctgggtggc atggtatggc gtgcagacac
      361 taaatccaac gtttatggta aaaaccacga caccggcgtt tctccggtct tcgctggcgg
      421 tgttgagtac gcgatcactc ctgaaatcgc tacccgtctg gaataccagt ggaccaacaa
      481 catcggtgac gcacacacca tcggcactcg tccggacaac ggcggaggtt ctggaggagg
      541 gagctctact agatggcgct cctgctgtgc ttcgtgctcc tgtgcggagt agtggatttc
      601 gccagaagtt tgagtatcac tactcctgaa gagatgattg aaaaagccaa aggggaaact
      661 gcctatctgc cgtgcaaatt tacgcttagt cccgaagacc agggaccgct ggacatcgag
      721 tggctgatat caccagctga taatcagaag gtggatcaag tgattatttt atattctgga
      781 gacaaaattt atgatgacta ctatccagat ctgaaaggcc gagtacattt tacgagtaat
      841 gatctcaaat ctggtgatgc atcaataaat gtaacgaatt tacaactgtc agatattggc
      901 acatatcagt gcaaagtgaa aaaagctcct ggtgttgcaa ataagaagat tcatctggta
      961 gttcttgtta agccttcagg tgcgagatgt tacgttgatg gatctgaaga aattggaagt
     1021 gactttaaga taaaatgtga accaaaagaa ggttcacttc cattacagta tgagtggcaa
     1081 aaattgtctg actcacagaa aatgcccact tcatggttag cagaaatgac ttcatctgtt
     1141 atatctgtaa aaaatgcctc ttctgagtac tctgggacat acagctgtac agtcagaaac
     1201 agagtgggct ctgatcagtg cctgttgcgt ctaaacgttg tccctccttc aaataaagct
     1261 ggactaattg caggagccat tataggaact ttgcttgctc tagcgctcat tggtcttatc
     1321 atcttttgct gtcgtaaaaa gcgcagagaa gaaaaatatg aaaaggaagt tcatcacgat
     1381 atcagggaag atgtgccacc tccaaagagc cgtacgtcca ctgccagaag ctacatcggc
     1441 agtaatcatt catccctggg gtccatgtct ccttccaaca tggaaggata ttccaagact
     1501 cagtataacc aagtaccaag tgaagacttt gaacgcactc ctcagagtcc gactctccca
     1561 cctgctaagg tagctgcccc taatctaagt cgaatgggtg cgattcctgt gatgattcca
     1621 gcacagagca aggatgggtc tatagtatag tactagagcc aggcatcaaa taaaacgaaa
     1681 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1741 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//