LOCUS BBa_K195303 5447 bp DNA linear 01-Nov-2024 DEFINITION pTet, RBS, neu gene operon and terminator. ACCESSION BBa_K195303 VERSION BBa_K195303.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..5310 /label=BBa_K195300 stem_loop 5330..5373 /label=stem_loop misc_feature 5319..5398 /label=BBa_B0010 terminator 5319..5398 /label=BBa_B0010 stem_loop 5414..5433 /label=T7 TE misc_feature 5440..5440 /label=stop polyA_site 5434..5447 /label=polya misc_feature 5407..5447 /label=BBa_B0012 terminator 5407..5447 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgagtaaaa agttaataat atttggtgcg ggtggttttt 121 caaaatctat aattgacagc ttaaatcata aacattacga gttaatagga tttatcgata 181 aatataaaag tggttatcat caatcatatc caatattagg taatgatatt gcagacatcg 241 agaataagga taattattat tattttattg ggataggcaa accatcaact aggaagcact 301 atttaaacat cataagaaaa cataatctac gcttaattaa cattatagat aaaactgcta 361 ttctatcacc aaatattata ctgggtgatg gaatttttat tggtaaaatg tgtatactta 421 accgtgatac tagaatacat gatgccgttg taataaatac taggagttta attgaacatg 481 gtaatgaaat aggctgctgt agcaatatct ctactaatgt tgtacttaat ggtgatgttt 541 ctgttggaga agaaactttt gttggtagct gtactgttgt aaatggccag ttgaagctag 601 gctcaaagag tattattggt tctgggtcgg ttgtaattag aaatatacca agtaatgttg 661 tagttgctgg gactccaaca agattaatta gggggaatga atgagtaata tatatatcgt 721 tgctgaaatt ggttgcaacc ataatggtag tgttgatatt gcaagagaaa tgatattaaa 781 agccaaagag gccggtgtta atgcagtaaa attccaaaca tttaaagctg ataaattaat 841 ttcagctatt gcacctaagg cagagtatca aataaaaaac acaggagaat tagaatctca 901 gttagaaatg acaaaaaagc ttgaaatgaa gaatcactat tatctccatc taatggaata 961 tgcagtcagt ttaaatttag atgttttttc tacccctttt gacgaagact ctattgattt 1021 tttagcatct ttgaaacaaa aaatatggaa aatcccttca ggtgagttat tgaatttacc 1081 gtatcttgaa aaaatagcca agcttccgat ccctgataag aaaataatca tatcaacagg 1141 aatggctact attgatgaga taaaacagtc tgtttctatt tttataaata ataaagttcc 1201 ggttggtaat attacaatat tacattgcaa tactgaatat ccaacgccct ttgaggatgt 1261 aaaccttaat gctattaatg atttgaaaaa acacttccct aagaataaca taggcttctc 1321 tgatcattct agcgggtttt atgcagctat tgcggcggtg ccttatggaa taacttttat 1381 tgaaaaacat ttcactttag ataaatctat gtctggccca gatcatttgg cctcaataga 1441 acctgatgaa ctgaaacatc tttgtattgg ggtcaggtgt gttgaaaaat ctttaggttc 1501 aaatagtaaa gtggttacag cttcagaaag gaagaataaa atcgtagcaa gaaagtctat 1561 tatagctaaa acagagataa aaaaaggtga ggttttttca gaaaaaaata taacaacaaa 1621 aagacctggt aatggtatca gtccgatgga gtggtataat ttattgggta aaattgcaga 1681 gcaagacttt attccagatg aattaataat tcatagcgaa ttcaaaaatc agggggaata 1741 atgagaacaa aaattattgc gataattcca gcccgtagtg gatctaaagg gttgagaaat 1801 aaaaatgctt tgatgctgat agataaacct cttcttgctt atacaattga agctgccttg 1861 cagtcagaaa tgtttgagaa agtaattgtg acaactgact ccgaacagta tggagcaata 1921 gcagagtcat atggtgctga ttttttgctg agaccggaag aactagcaac tgataaagca 1981 tcatcatttg aatttataaa acatgcgtta agtatatata ctgattatga gagctttgct 2041 ttattacaac caacttcacc ctttagagat tcgacccata ttattgaggc tgtaaagtta 2101 tatcaaactt tagaaaaata ccaatgtgtt gtttctgtta ctagaagcaa taagccatca 2161 caaataatta gaccattaga tgattactcg acactgtctt tttttgacct tgattatagt 2221 aaatataatc gaaactcaat agtagaatat catccgaatg gagctatatt tatagctaat 2281 aagcagcatt atcttcatac aaagcatttt tttggtcgct attcactagc ttatattatg 2341 gataaggaaa gctctttaga tatagatgat agaatggatt tcgaacttgc aattaccatt 2401 cagcaaaaaa aaaatagaca aaaaatactt tatcaaaaca tacataatag aatcaatgag 2461 aaacgaaatg aatttgatag tgtaagtgat ataactttaa ttggacactc gctgtttgat 2521 tattgggacg taaaaaaaat aaatgatata gaagttaata acttaggtat cgctggtata 2581 aactcgaagg agtactatga atatattatt gagaaagagc tgattgttaa tttcggagag 2641 tttgttttca tcttttttgg aactaatgat atagttgtta gtgattggaa aaaagaagac 2701 acattgtggt atttgaagaa aacatgccag tatataaaga agaaaaatgc tgcatcaaaa 2761 atttatttat tgtcggttcc tcctgttttt gggcgtattg atcgagataa tagaataatt 2821 aatgatttaa attcttatct tcgagagaat gtagattttg cgaagtttat tagcttggat 2881 cacgttttaa aagactctta tggcaatcta aataaaatgt atacttatga tggcttacat 2941 tttaatagta atgggtatac agtattagaa aacgaaatag cggagattgt taaatgaaaa 3001 aaatattata cgtaactgga tctagagctg aatatggaat agttcggaga cttttgacaa 3061 tgctaagaga aactccagaa atacagcttg atttggcagt tacaggaatg cattgtgata 3121 atgcgtatgg aaatacaata catattatag aacaagataa ttttaatatt atcaaggttg 3181 tggatataaa tatcaataca acttcacata ctcacattct ccattcaatg agtgtttgcc 3241 tcaattcgtt tggtgatttt ttttcaaata acacatatga tgcggttatg gttttaggcg 3301 atagatatga aatattttca gtcgctatcg cagcatcaat gcataatatt ccattaattc 3361 atattcatgg tggtgaaaag acattagcta attatgatga gtttattagg cattcaatta 3421 ctaaaatgag taaactccat cttacttcta cagaagagta taaaaaacga gtaattcaac 3481 taggtgaaaa gcctggtagt gtgtttaata ttggttctct tggtgcagaa aatgctcttt 3541 cattgcattt accaaataag caggagttgg aactaaaata tggttcactg ttaaaacggt 3601 actttgttgt agtattccat cctgaaacac tttccacgca gtcggttaat gatcaaatag 3661 atgagttatt gtcagcgatt tcttttttta aaaatactca cgactttatt tttattggca 3721 gtaacgctga cactggttct gatataattc agagaaaagt aaaatatttt tgcaaagagt 3781 ataagttcag atatttgatt tctattcgtt cagaagatta tttggcaatg attaaatact 3841 cttgtgggct aattgggaac tcctcctctg gtttaattga ggttccatct ttaaaagttg 3901 caacaattaa cattggtgat aggcagaaag gccgtgttcg tggagccagt gtaatagatg 3961 tacccgttga aaaaaatgca atcgtcagag ggataaatat atctcaagat gaaaaattta 4021 ttagtgttgt acagtcatct agtaatcctt attttaaaga aaatgcttta attaatgctg 4081 ttagaattat taaggatttt attaaatcaa aaaataaaga ttacaaagat ttttatgaca 4141 tcccggaatg taccaccagt tatgactaga aaaaaagtgc tttgttttgt ctttcgttat 4201 gattctcatt ttttagcttt gaaaaatatt tttgagcaga tagatgttga ttcatatgat 4261 ttattttttt gctgcttgga taattctcta caagagtttg taaaaaaaaa tttagatgaa 4321 aagatagttg tattctatcc tgatgacttt gtttgttttt tcacttttat taatattgag 4381 tttatttttt gttcaacagg agggaaggac cttcatgaaa ttgttaatac tgtaagaaca 4441 aaagatacca taattatatc ttgttttcct ggcattgtcc ttacttctca gatagaagct 4501 tttatttcaa aatctaatag tcactattta cttattaact ccccaaaaga tattaaaacg 4561 tataaaaaaa tttgtaaaat aataggggtt ccttttaatg gaattctttt tggtccacca 4621 tggattaaaa atgtcaatat caatgcaaaa agtgagaatt cttgtcttat cgttgatcaa 4681 gttaatgaac ccttgacgcc aataaagagg atagaatatg cacgtttttt gattagagta 4741 attcagaaac atccacatat gaattttatt tttaaaactc gaaatcctct tatatcacca 4801 gactcaattg tttttgatat taaggaatac attgaacgct tcgatttgaa aaatataaca 4861 tttagcgatg ataatattga ttctttaatt tctaaagttg aatattgtat tacaatatct 4921 tcttcggtcg caatatattg tctggctaat aaaattaagg tttatttaat aaatggattt 4981 aatcatactt gcaatggaca atgttatttt tcaagatctg gacttattgt tgactataat 5041 aagtttaatt ttaaacacat tccacgtatt aaaaaaaaat ggatggagga gaacttttat 5101 tactctaggg atattcaaca taagattttg aatgatattt taaaaatgcc gccaaatgtt 5161 aatgttagga cttttggaat taaaagatct acattgatta tattgttttt gatctttttt 5221 aatttctttt tctcattagg accaaaaaaa ataaaaacat tgaaaaaaat ccataaagtt 5281 ttattaaggt ataagaaaga tgatatttga tactagagcc aggcatcaaa taaaacgaaa 5341 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 5401 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //