BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1690 1 polya range1690 1 28 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K195306 1 BBa_K195306 RBS,sialyltransferase 0160 and terminator 2009-10-20T11:00:00Z 2015-05-08T01:11:16Z Photobacterium damsela alpha-2,6-sialyltransferase can transfer polysaccharides onto the cell membrane to fuse with the membrane proteins, which then form glycoproteins. false false _292_ 0 4842 9 Not in stock false None false Tung Chao component2059145 1 BBa_B0010 component2059147 1 BBa_B0012 component2059143 1 BBa_B0034 component2059144 1 BBa_K195304 annotation2059143 1 BBa_B0034 range2059143 1 1 12 annotation2059144 1 BBa_K195304 range2059144 1 19 2046 annotation2059147 1 BBa_B0012 range2059147 1 2143 2183 annotation2059145 1 BBa_B0010 range2059145 1 2055 2134 BBa_K195304 1 BBa_K195304 Sialyltransferase 0160 2009-10-20T11:00:00Z 2015-05-08T01:11:16Z Photobacterium damsela alpha-2,6-sialyltransferase can transfer polysaccharides onto the cell membrane to fuse with the membrane proteins, which then form glycoproteins. false false _292_ 0 4842 9 Not in stock false None false Tung Chao BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K195304_sequence 1 atgaagaaaatactgacagttctatctatttttattctttcagcgtgtaatagtgacaataccagcttgaaagaaacggtaagctctaattctgcagatgtagtagaaacagaaacttaccaactgacaccgattgatgctcctagctcttttttatctcattcttgggagcaaacatgtggcacacctatcttgaatgaaagtgacaagcaagcgatatcttttgattttgttgctccagagttaaagcaagatgaaaagtattgttttacttttaaaggtattacaggcgatcataggtatatcacaaatacaacattaactgttgttgcacctacgctagaagtttacatcgatcatgcatccttaccatcgctacagcagcttatccacattattcaagcaaaagatgaatacccaagtaatcaacgttttgtctcttggaagcgtgtaactgttgatgctgataatgccaataagttaaacattcatacttatccattaaaaggcaataatacctcaccagaaatggtggcagcgattgatgagtatgctcagagcaaaaatcgattgaatatagagttctatacaaatacagctcatgtttttaataatttaccacctattattcaacctttatataataacgagaaggtgaaaatttctcatattagtttgtatgatgatggttcttctgaatatgtaagtttatatcaatggaaagatacaccaaataagatagaaacattagaaggtgaagtatcgcttcttgctaattatttagcaggaacatctccggatgcaccaaaaggaatgggaaatcgttataactggcataaattatatgacactgattattactttttgcgcgaagattaccttgacgttgaagcaaacctacatgatttacgtgattatttaggctcttccgcaaagcaaatgccatgggatgaatttgctaaattatctgattctcagcaaacactatttttagatattgtgggttttgataaagagcaattgcaacaacaatattcacaatccccactaccaaactttatttttaccggcacaacaacttgggctgggggggaaacgaaagagtattatgctcagcaacaagtaaatgtgattaataatgcgatcaatgaaactagcccttattatttaggtaaagactacgatctatttttcaaggggcatcctgctggtggcgttattaacgacatcattcttggaagcttccctgatatgatcaatattccagccaagatttcatttgaggtcttgatgatgacggatatgttgcctgatacagtagctggtattgcgagctctctgtacttcacaattcctgccgataaagttaattttattgtatttacttcatctgacactattactgatcgtgaagaggctcttaaatcaccattagtacaagtgatgctaacgttgggtattgttaaagaaaaagatgttctgttctgggctgatcataaagtaaactcgatggaagttgccattgatgaagcctgtactcggatcattgcaaagcgacaaccaaccgcgagtgatttacgcttggttattgctattatcaaaacaattactgatcttgagcgtattggcgatgtggcagaaagtattgctaaagtcgcattagagagctttagtaataagcaatataacctattggtttctttagaatctcttggccagcatacggttcgaatgctgcatgaggtgttagatgcgtttgctcgtatggatgttaaagccgcaatagaagtgtaccaagaagatgatcgaattgatcaagagtatgagtcgatagtcagacagctaatggcccatatgatggaagatccaagctcaattcctaatgtaatgaaagtgatgtgggcggcacgttctattgagcgagtgggtgatcgctgtcaaaacatttgtgagtacattatctactttgtgaagggtaaagacgttcgccataccaaaccagatgattttggtactatgctcgattaa BBa_K195306_sequence 1 aaagaggagaaatactagatgaagaaaatactgacagttctatctatttttattctttcagcgtgtaatagtgacaataccagcttgaaagaaacggtaagctctaattctgcagatgtagtagaaacagaaacttaccaactgacaccgattgatgctcctagctcttttttatctcattcttgggagcaaacatgtggcacacctatcttgaatgaaagtgacaagcaagcgatatcttttgattttgttgctccagagttaaagcaagatgaaaagtattgttttacttttaaaggtattacaggcgatcataggtatatcacaaatacaacattaactgttgttgcacctacgctagaagtttacatcgatcatgcatccttaccatcgctacagcagcttatccacattattcaagcaaaagatgaatacccaagtaatcaacgttttgtctcttggaagcgtgtaactgttgatgctgataatgccaataagttaaacattcatacttatccattaaaaggcaataatacctcaccagaaatggtggcagcgattgatgagtatgctcagagcaaaaatcgattgaatatagagttctatacaaatacagctcatgtttttaataatttaccacctattattcaacctttatataataacgagaaggtgaaaatttctcatattagtttgtatgatgatggttcttctgaatatgtaagtttatatcaatggaaagatacaccaaataagatagaaacattagaaggtgaagtatcgcttcttgctaattatttagcaggaacatctccggatgcaccaaaaggaatgggaaatcgttataactggcataaattatatgacactgattattactttttgcgcgaagattaccttgacgttgaagcaaacctacatgatttacgtgattatttaggctcttccgcaaagcaaatgccatgggatgaatttgctaaattatctgattctcagcaaacactatttttagatattgtgggttttgataaagagcaattgcaacaacaatattcacaatccccactaccaaactttatttttaccggcacaacaacttgggctgggggggaaacgaaagagtattatgctcagcaacaagtaaatgtgattaataatgcgatcaatgaaactagcccttattatttaggtaaagactacgatctatttttcaaggggcatcctgctggtggcgttattaacgacatcattcttggaagcttccctgatatgatcaatattccagccaagatttcatttgaggtcttgatgatgacggatatgttgcctgatacagtagctggtattgcgagctctctgtacttcacaattcctgccgataaagttaattttattgtatttacttcatctgacactattactgatcgtgaagaggctcttaaatcaccattagtacaagtgatgctaacgttgggtattgttaaagaaaaagatgttctgttctgggctgatcataaagtaaactcgatggaagttgccattgatgaagcctgtactcggatcattgcaaagcgacaaccaaccgcgagtgatttacgcttggttattgctattatcaaaacaattactgatcttgagcgtattggcgatgtggcagaaagtattgctaaagtcgcattagagagctttagtaataagcaatataacctattggtttctttagaatctcttggccagcatacggttcgaatgctgcatgaggtgttagatgcgtttgctcgtatggatgttaaagccgcaatagaagtgtaccaagaagatgatcgaattgatcaagagtatgagtcgatagtcagacagctaatggcccatatgatggaagatccaagctcaattcctaatgtaatgaaagtgatgtgggcggcacgttctattgagcgagtgggtgatcgctgtcaaaacatttgtgagtacattatctactttgtgaagggtaaagacgttcgccataccaaaccagatgattttggtactatgctcgattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z