LOCUS       BBa_K195307             2245 bp    DNA     linear       26-Nov-2024
DEFINITION  pTet, RBS, sialyltransferase 0160 and terminator
ACCESSION   BBa_K195307
VERSION     BBa_K195307.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..2108
                     /label=BBa_K195304
     stem_loop       2128..2171
                     /label=stem_loop
     misc_feature    2117..2196
                     /label=BBa_B0010
     terminator      2117..2196
                     /label=BBa_B0010
     stem_loop       2212..2231
                     /label=T7 TE
     misc_feature    2238..2238
                     /label=stop
     polyA_site      2232..2245
                     /label=polya
     misc_feature    2205..2245
                     /label=BBa_B0012
     terminator      2205..2245
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaagaaaa tactgacagt tctatctatt tttattcttt
      121 cagcgtgtaa tagtgacaat accagcttga aagaaacggt aagctctaat tctgcagatg
      181 tagtagaaac agaaacttac caactgacac cgattgatgc tcctagctct tttttatctc
      241 attcttggga gcaaacatgt ggcacaccta tcttgaatga aagtgacaag caagcgatat
      301 cttttgattt tgttgctcca gagttaaagc aagatgaaaa gtattgtttt acttttaaag
      361 gtattacagg cgatcatagg tatatcacaa atacaacatt aactgttgtt gcacctacgc
      421 tagaagttta catcgatcat gcatccttac catcgctaca gcagcttatc cacattattc
      481 aagcaaaaga tgaataccca agtaatcaac gttttgtctc ttggaagcgt gtaactgttg
      541 atgctgataa tgccaataag ttaaacattc atacttatcc attaaaaggc aataatacct
      601 caccagaaat ggtggcagcg attgatgagt atgctcagag caaaaatcga ttgaatatag
      661 agttctatac aaatacagct catgttttta ataatttacc acctattatt caacctttat
      721 ataataacga gaaggtgaaa atttctcata ttagtttgta tgatgatggt tcttctgaat
      781 atgtaagttt atatcaatgg aaagatacac caaataagat agaaacatta gaaggtgaag
      841 tatcgcttct tgctaattat ttagcaggaa catctccgga tgcaccaaaa ggaatgggaa
      901 atcgttataa ctggcataaa ttatatgaca ctgattatta ctttttgcgc gaagattacc
      961 ttgacgttga agcaaaccta catgatttac gtgattattt aggctcttcc gcaaagcaaa
     1021 tgccatggga tgaatttgct aaattatctg attctcagca aacactattt ttagatattg
     1081 tgggttttga taaagagcaa ttgcaacaac aatattcaca atccccacta ccaaacttta
     1141 tttttaccgg cacaacaact tgggctgggg gggaaacgaa agagtattat gctcagcaac
     1201 aagtaaatgt gattaataat gcgatcaatg aaactagccc ttattattta ggtaaagact
     1261 acgatctatt tttcaagggg catcctgctg gtggcgttat taacgacatc attcttggaa
     1321 gcttccctga tatgatcaat attccagcca agatttcatt tgaggtcttg atgatgacgg
     1381 atatgttgcc tgatacagta gctggtattg cgagctctct gtacttcaca attcctgccg
     1441 ataaagttaa ttttattgta tttacttcat ctgacactat tactgatcgt gaagaggctc
     1501 ttaaatcacc attagtacaa gtgatgctaa cgttgggtat tgttaaagaa aaagatgttc
     1561 tgttctgggc tgatcataaa gtaaactcga tggaagttgc cattgatgaa gcctgtactc
     1621 ggatcattgc aaagcgacaa ccaaccgcga gtgatttacg cttggttatt gctattatca
     1681 aaacaattac tgatcttgag cgtattggcg atgtggcaga aagtattgct aaagtcgcat
     1741 tagagagctt tagtaataag caatataacc tattggtttc tttagaatct cttggccagc
     1801 atacggttcg aatgctgcat gaggtgttag atgcgtttgc tcgtatggat gttaaagccg
     1861 caatagaagt gtaccaagaa gatgatcgaa ttgatcaaga gtatgagtcg atagtcagac
     1921 agctaatggc ccatatgatg gaagatccaa gctcaattcc taatgtaatg aaagtgatgt
     1981 gggcggcacg ttctattgag cgagtgggtg atcgctgtca aaacatttgt gagtacatta
     2041 tctactttgt gaagggtaaa gacgttcgcc ataccaaacc agatgatttt ggtactatgc
     2101 tcgattaata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     2161 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     2221 tcgggtgggc ctttctgcgt ttata
//