BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1690 1 polya range1690 1 28 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986786 1 TetR 2 range1986786 1 26 44 annotation1986787 1 -10 range1986787 1 43 48 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986785 1 -35 range1986785 1 20 25 BBa_K195304 1 BBa_K195304 Sialyltransferase 0160 2009-10-20T11:00:00Z 2015-05-08T01:11:16Z Photobacterium damsela alpha-2,6-sialyltransferase can transfer polysaccharides onto the cell membrane to fuse with the membrane proteins, which then form glycoproteins. false false _292_ 0 4842 9 Not in stock false None false Tung Chao BBa_K195307 1 BBa_K195307 pTet, RBS, sialyltransferase 0160 and terminator 2009-10-20T11:00:00Z 2015-05-08T01:11:16Z Photobacterium damsela The pTet promoter is strong enough to overexpress the sialyltransferase 0160. false false _292_ 0 4842 9 Not in stock false None false Tung Chao component2059170 1 BBa_R0040 component2059180 1 BBa_B0012 component2059176 1 BBa_B0034 component2059178 1 BBa_B0010 component2059177 1 BBa_K195304 annotation2059176 1 BBa_B0034 range2059176 1 63 74 annotation2059180 1 BBa_B0012 range2059180 1 2205 2245 annotation2059178 1 BBa_B0010 range2059178 1 2117 2196 annotation2059177 1 BBa_K195304 range2059177 1 81 2108 annotation2059170 1 BBa_R0040 range2059170 1 1 54 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_K195304_sequence 1 atgaagaaaatactgacagttctatctatttttattctttcagcgtgtaatagtgacaataccagcttgaaagaaacggtaagctctaattctgcagatgtagtagaaacagaaacttaccaactgacaccgattgatgctcctagctcttttttatctcattcttgggagcaaacatgtggcacacctatcttgaatgaaagtgacaagcaagcgatatcttttgattttgttgctccagagttaaagcaagatgaaaagtattgttttacttttaaaggtattacaggcgatcataggtatatcacaaatacaacattaactgttgttgcacctacgctagaagtttacatcgatcatgcatccttaccatcgctacagcagcttatccacattattcaagcaaaagatgaatacccaagtaatcaacgttttgtctcttggaagcgtgtaactgttgatgctgataatgccaataagttaaacattcatacttatccattaaaaggcaataatacctcaccagaaatggtggcagcgattgatgagtatgctcagagcaaaaatcgattgaatatagagttctatacaaatacagctcatgtttttaataatttaccacctattattcaacctttatataataacgagaaggtgaaaatttctcatattagtttgtatgatgatggttcttctgaatatgtaagtttatatcaatggaaagatacaccaaataagatagaaacattagaaggtgaagtatcgcttcttgctaattatttagcaggaacatctccggatgcaccaaaaggaatgggaaatcgttataactggcataaattatatgacactgattattactttttgcgcgaagattaccttgacgttgaagcaaacctacatgatttacgtgattatttaggctcttccgcaaagcaaatgccatgggatgaatttgctaaattatctgattctcagcaaacactatttttagatattgtgggttttgataaagagcaattgcaacaacaatattcacaatccccactaccaaactttatttttaccggcacaacaacttgggctgggggggaaacgaaagagtattatgctcagcaacaagtaaatgtgattaataatgcgatcaatgaaactagcccttattatttaggtaaagactacgatctatttttcaaggggcatcctgctggtggcgttattaacgacatcattcttggaagcttccctgatatgatcaatattccagccaagatttcatttgaggtcttgatgatgacggatatgttgcctgatacagtagctggtattgcgagctctctgtacttcacaattcctgccgataaagttaattttattgtatttacttcatctgacactattactgatcgtgaagaggctcttaaatcaccattagtacaagtgatgctaacgttgggtattgttaaagaaaaagatgttctgttctgggctgatcataaagtaaactcgatggaagttgccattgatgaagcctgtactcggatcattgcaaagcgacaaccaaccgcgagtgatttacgcttggttattgctattatcaaaacaattactgatcttgagcgtattggcgatgtggcagaaagtattgctaaagtcgcattagagagctttagtaataagcaatataacctattggtttctttagaatctcttggccagcatacggttcgaatgctgcatgaggtgttagatgcgtttgctcgtatggatgttaaagccgcaatagaagtgtaccaagaagatgatcgaattgatcaagagtatgagtcgatagtcagacagctaatggcccatatgatggaagatccaagctcaattcctaatgtaatgaaagtgatgtgggcggcacgttctattgagcgagtgggtgatcgctgtcaaaacatttgtgagtacattatctactttgtgaagggtaaagacgttcgccataccaaaccagatgattttggtactatgctcgattaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K195307_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaagaaaatactgacagttctatctatttttattctttcagcgtgtaatagtgacaataccagcttgaaagaaacggtaagctctaattctgcagatgtagtagaaacagaaacttaccaactgacaccgattgatgctcctagctcttttttatctcattcttgggagcaaacatgtggcacacctatcttgaatgaaagtgacaagcaagcgatatcttttgattttgttgctccagagttaaagcaagatgaaaagtattgttttacttttaaaggtattacaggcgatcataggtatatcacaaatacaacattaactgttgttgcacctacgctagaagtttacatcgatcatgcatccttaccatcgctacagcagcttatccacattattcaagcaaaagatgaatacccaagtaatcaacgttttgtctcttggaagcgtgtaactgttgatgctgataatgccaataagttaaacattcatacttatccattaaaaggcaataatacctcaccagaaatggtggcagcgattgatgagtatgctcagagcaaaaatcgattgaatatagagttctatacaaatacagctcatgtttttaataatttaccacctattattcaacctttatataataacgagaaggtgaaaatttctcatattagtttgtatgatgatggttcttctgaatatgtaagtttatatcaatggaaagatacaccaaataagatagaaacattagaaggtgaagtatcgcttcttgctaattatttagcaggaacatctccggatgcaccaaaaggaatgggaaatcgttataactggcataaattatatgacactgattattactttttgcgcgaagattaccttgacgttgaagcaaacctacatgatttacgtgattatttaggctcttccgcaaagcaaatgccatgggatgaatttgctaaattatctgattctcagcaaacactatttttagatattgtgggttttgataaagagcaattgcaacaacaatattcacaatccccactaccaaactttatttttaccggcacaacaacttgggctgggggggaaacgaaagagtattatgctcagcaacaagtaaatgtgattaataatgcgatcaatgaaactagcccttattatttaggtaaagactacgatctatttttcaaggggcatcctgctggtggcgttattaacgacatcattcttggaagcttccctgatatgatcaatattccagccaagatttcatttgaggtcttgatgatgacggatatgttgcctgatacagtagctggtattgcgagctctctgtacttcacaattcctgccgataaagttaattttattgtatttacttcatctgacactattactgatcgtgaagaggctcttaaatcaccattagtacaagtgatgctaacgttgggtattgttaaagaaaaagatgttctgttctgggctgatcataaagtaaactcgatggaagttgccattgatgaagcctgtactcggatcattgcaaagcgacaaccaaccgcgagtgatttacgcttggttattgctattatcaaaacaattactgatcttgagcgtattggcgatgtggcagaaagtattgctaaagtcgcattagagagctttagtaataagcaatataacctattggtttctttagaatctcttggccagcatacggttcgaatgctgcatgaggtgttagatgcgtttgctcgtatggatgttaaagccgcaatagaagtgtaccaagaagatgatcgaattgatcaagagtatgagtcgatagtcagacagctaatggcccatatgatggaagatccaagctcaattcctaatgtaatgaaagtgatgtgggcggcacgttctattgagcgagtgggtgatcgctgtcaaaacatttgtgagtacattatctactttgtgaagggtaaagacgttcgccataccaaaccagatgattttggtactatgctcgattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z