LOCUS BBa_K1954001 3532 bp DNA linear 25-Nov-2024 DEFINITION Lycopene cassette under the control of NarK, an oxidative stress inducible promoter ACCESSION BBa_K1954001 VERSION BBa_K1954001.1 FEATURES Location/Qualifiers misc_feature 4..16 /label=IHF promoter 126..133 /label=-10 promoter 98..106 /label=-35 misc_feature 92..105 /label=Fnr misc_feature 88..106 /label=Fis misc_feature 70..76 /label=NarL misc_feature 34..45 /label=NarL misc_feature 54..67 /label=Fnr misc_feature 57..63 /label=NarL misc_feature 47..53 /label=NarL promoter 1..139 /label=BBa_K239005 RBS 148..151 /label=BBa_K118012 CDS 158..1069 /label=BBa_I742151 misc_feature 1078..1080 /label=SacI RBS 1081..1087 /label=rbs RBS 1078..1087 /label=BBa_J15001 CDS 1094..2023 /label=crtB coding sequence CDS 1094..2026 /label=BBa_K118002 misc_feature 2035..2037 /label=SacI RBS 2038..2044 /label=rbs RBS 2035..2044 /label=BBa_J15001 misc_feature 2528..2533 /label=PstI site removed misc_feature 2939..2944 /label=PstI site removed CDS 2051..3529 /label=crtI coding sequence CDS 2051..3532 /label=BBa_K118003 misc_feature 148..3532 /label=BBa_K274100 ORIGIN 1 gcaccgtgaa aaatctcata atttttatga agtcactgta ctcactatgg gtaatgataa 61 atatcaatga tagataaagt tatcttatcg tttgatttac atcaaattgc ctttagctac 121 agacactaag gtggcagact actagaggag gtactagatg acggtctgcg caaaaaaaca 181 cgttcatctc actcgcgatg ctgcggagca gttactggct gatattgatc gacgccttga 241 tcagttattg cccgtggagg gagaacggga tgttgtgggt gccgcgatgc gtgaaggtgc 301 gctggcaccg ggaaaacgta ttcgccccat gttgctgttg ctgaccgccc gcgatctggg 361 ttgcgctgtc agccatgacg gattactgga tttggcctgt gcggtggaaa tggtccacgc 421 ggcttcgctg atccttgacg atatgccctg catggacgat gcgaagctgc ggcgcggacg 481 ccctaccatt cattctcatt acggagagca tgtggcaata ctggcggcgg ttgccttgct 541 gagtaaagcc tttggcgtaa ttgccgatgc agatggcctc acgccgctgg caaaaaatcg 601 ggcggtttct gaactgtcaa acgccatcgg catgcaagga ttggttcagg gtcagttcaa 661 ggatctgtct gaaggggata agccgcgcag cgctgaagct attttgatga cgaatcactt 721 taaaaccagc acgctgtttt gtgcctccat gcagatggcc tcgattgttg cgaatgcctc 781 cagcgaagcg cgtgattgcc tgcatcgttt ttcacttgat cttggtcagg catttcaact 841 gctggacgat ttgaccgatg gcatgaccga caccggtaag gatagcaatc aggacgccgg 901 taaatcgacg ctggtcaatc tgttaggccc gagggcggtt gaagaacgtc tgagacaaca 961 tcttcagctt gccagtgagc atctctctgc ggcctgccaa cacgggcacg ccactcaaca 1021 ttttattcag gcctggtttg acaaaaaact cgctgccgtc agttaataat actagagctc 1081 aaggaggtac tagatgaata atccgtcgtt actcaatcat gcggtcgaaa cgatggcagt 1141 tggctcgaaa agttttgcga cagcctcaaa gttatttgat gcaaaaaccc ggcgcagcgt 1201 actgatgctc tacgcctggt gccgccattg tgacgatgtt attgacgatc agacgctggg 1261 ctttcaggcc cggcagcctg ccttacaaac gcccgaacaa cgtctgatgc aacttgagat 1321 gaaaacgcgc caggcctatg caggatcgca gatgcacgaa ccggcgtttg cggcttttca 1381 ggaagtggct atggctcatg atatcgcccc ggcttacgcg tttgatcatc tggaaggctt 1441 cgccatggat gtacgcgaag cgcaatacag ccaactggat gatacgctgc gctattgcta 1501 tcacgttgca ggcgttgtcg gcttgatgat ggcgcaaatc atgggcgtgc gggataacgc 1561 cacgctggac cgcgcctgtg accttgggct ggcatttcag ttgaccaata ttgctcgcga 1621 tattgtggac gatgcgcatg cgggccgctg ttatctgccg gcaagctggc tggagcatga 1681 aggtctgaac aaagagaatt atgcggcacc tgaaaaccgt caggcgctga gccgtatcgc 1741 ccgtcgtttg gtgcaggaag cagaacctta ctatttgtct gccacagccg gcctggcagg 1801 gttgcccctg cgttccgcct gggcaatcgc tacggcgaag caggtttacc ggaaaatagg 1861 tgtcaaagtt gaacaggccg gtcagcaagc ctgggatcag cggcagtcaa cgaccacgcc 1921 cgaaaaatta acgctgctgc tggccgcctc tggtcaggcc cttacttccc ggatgcgggc 1981 tcatcctccc cgccctgcgc atctctggca gcgcccgctc taataatact agagctcaag 2041 gaggtactag atgaaaccaa ctacggtaat tggtgcaggc ttcggtggcc tggcactggc 2101 aattcgtcta caagctgcgg ggatccccgt cttactgctt gaacaacgtg ataaacccgg 2161 cggtcgggct tatgtctacg aggatcaggg gtttaccttt gatgcaggcc cgacggttat 2221 caccgatccc agtgccattg aagaactgtt tgcactggca ggaaaacagt taaaagagta 2281 tgtcgaactg ctgccggtta cgccgtttta ccgcctgtgt tgggagtcag ggaaggtctt 2341 taattacgat aacgatcaaa cccggctcga agcgcagatt cagcagttta atccccgcga 2401 tgtcgaaggt tatcgtcagt ttctggacta ttcacgcgcg gtgtttaaag aaggctatct 2461 aaagctcggt actgtccctt ttttatcgtt cagagacatg cttcgcgccg cacctcaact 2521 ggcgaaactg caagcatgga gaagcgttta cagtaaggtt gccagttaca tcgaagatga 2581 acatctgcgc caggcgtttt ctttccactc gctgttggtg ggcggcaatc ccttcgccac 2641 ctcatccatt tatacgttga tacacgcgct ggagcgtgag tggggcgtct ggtttccgcg 2701 tggcggcacc ggcgcattag ttcaggggat gataaagctg tttcaggatc tgggtggcga 2761 agtcgtgtta aacgccagag tcagccatat ggaaacgaca ggaaacaaga ttgaagccgt 2821 gcatttagag gacggtcgca ggttcctgac gcaagccgtc gcgtcaaatg cagatgtggt 2881 tcatacctat cgcgacctgt taagccagca ccctgccgcg gttaagcagt ccaacaaact 2941 gcaaactaag cgcatgagta actctctgtt tgtgctctat tttggtttga atcaccatca 3001 tgatcagctc gcgcatcaca cggtttgttt cggcccgcgt taccgcgagc tgattgacga 3061 aatttttaat catgatggcc tcgcagagga cttctcactt tatctgcacg cgccctgtgt 3121 cacggattcg tcactggcgc ctgaaggttg cggcagttac tatgtgttgg cgccggtgcc 3181 gcatttaggc accgcgaacc tcgactggac ggttgagggg ccaaaactac gcgaccgtat 3241 ttttgcgtac cttgagcagc attacatgcc tggcttacgg agtcagctgg tcacgcaccg 3301 gatgtttacg ccgtttgatt ttcgcgacca gcttaatgcc tatcatggct cagccttttc 3361 tgtggagccc gttcttaccc agagcgcctg gtttcggccg cataaccgcg ataaaaccat 3421 tactaatctc tacctggtcg gcgcaggcac gcatcccggc gcaggcattc ctggcgtcat 3481 cggctcggca aaagcgacag caggtttgat gctggaggat ctgatataat aa //