##gff-version 3
##sequence-region BBa_K1954001 1 3532
BBa_K1954001	.	promoter	1	139	.	+	0	ID=BBa_K239005;Name=BBa_K239005
BBa_K1954001	.	non_covalent_binding_site	47	53	.	+	0	ID=annotation2006719;Name=NarL;Parent=BBa_K239005
BBa_K1954001	.	non_covalent_binding_site	57	63	.	+	0	ID=annotation2006718;Name=NarL;Parent=BBa_K239005
BBa_K1954001	.	non_covalent_binding_site	54	67	.	+	0	ID=annotation2006717;Name=Fnr;Parent=BBa_K239005
BBa_K1954001	.	promoter	98	106	.	+	0	ID=annotation2006712;Name=-35;Parent=BBa_K239005
BBa_K1954001	.	non_covalent_binding_site	70	76	.	+	0	ID=annotation2006715;Name=NarL;Parent=BBa_K239005
BBa_K1954001	.	promoter	126	133	.	+	0	ID=annotation2006711;Name=-10;Parent=BBa_K239005
BBa_K1954001	.	non_covalent_binding_site	88	106	.	+	0	ID=annotation2006714;Name=Fis;Parent=BBa_K239005
BBa_K1954001	.	non_covalent_binding_site	92	105	.	+	0	ID=annotation2006713;Name=Fnr;Parent=BBa_K239005
BBa_K1954001	.	non_covalent_binding_site	34	45	.	+	0	ID=annotation2006716;Name=NarL;Parent=BBa_K239005
BBa_K1954001	.	non_covalent_binding_site	4	16	.	+	0	ID=annotation2006710;Name=IHF;Parent=BBa_K239005
BBa_K1954001	.	engineered_region	148	3532	.	+	0	ID=BBa_K274100;Name=BBa_K274100
BBa_K1954001	.	CDS	1094	2026	.	+	0	ID=BBa_K118002;Name=BBa_K118002;Parent=BBa_K274100
BBa_K1954001	.	CDS	1094	2023	.	+	0	ID=annotation1995701;Name=crtB coding sequence;Parent=BBa_K118002
BBa_K1954001	.	CDS	158	1069	.	+	0	ID=BBa_I742151;Name=BBa_I742151;Parent=BBa_K274100
BBa_K1954001	.	CDS	2051	3532	.	+	0	ID=BBa_K118003;Name=BBa_K118003;Parent=BBa_K274100
BBa_K1954001	.	point_mutation	2528	2533	.	+	0	ID=annotation1968244;Name=PstI site removed;Parent=BBa_K118003
BBa_K1954001	.	CDS	2051	3529	.	+	0	ID=annotation1995702;Name=crtI coding sequence;Parent=BBa_K118003
BBa_K1954001	.	point_mutation	2939	2944	.	+	0	ID=annotation1968245;Name=PstI site removed;Parent=BBa_K118003
BBa_K1954001	.	ribosome_entry_site	148	151	.	+	0	ID=BBa_K118012;Name=BBa_K118012;Parent=BBa_K274100
BBa_K1954001	.	ribosome_entry_site	1078	1087	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K274100
BBa_K1954001	.	ribosome_entry_site	1081	1087	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_K1954001	.	sequence_feature	1078	1080	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_K1954001	.	ribosome_entry_site	2035	2044	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K274100
BBa_K1954001	.	ribosome_entry_site	2038	2044	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_K1954001	.	sequence_feature	2035	2037	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
>BBa_K1954001
gcaccgtgaaaaatctcataatttttatgaagtcactgtactcactatgggtaatgataaatatcaatgatagataaag
ttatcttatcgtttgatttacatcaaattgcctttagctacagacactaaggtggcagactactagaggaggtactaga
tgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgcct
tgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaa
cgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttgg
cctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcg
cggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagccttt
ggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggca
tgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgac
gaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcg
cgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccg
acaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacg
tctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttatt
caggcctggtttgacaaaaaactcgctgccgtcagttaataatactagagctcaaggaggtactagatgaataatccgt
cgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaa
aacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggcttt
caggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcag
gatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtt
tgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctat
cacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtg
accttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgcc
ggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatc
gcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccg
cctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctggga
tcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatg
cgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataatactagagctcaaggaggtactagatga
aaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtctt
actgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccg
acggttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgc
tgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcga
agcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaa
gaaggctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaac
tgcaagcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttcca
ctcgctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggc
gtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcg
tgttaaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggtt
cctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggtt
aagcagtccaacaaactgcaaactaagcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatg
atcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatggcct
cgcagaggacttctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttac
tatgtgttggcgccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgta
tttttgcgtaccttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttga
ttttcgcgaccagcttaatgcctatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcgg
ccgcataaccgcgataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcg
tcatcggctcggcaaaagcgacagcaggtttgatgctggaggatctgatataataa