LOCUS BBa_K1954002 6650 bp DNA linear 22-Dec-2024 DEFINITION pGAP-Vitellogenin-GFP device ACCESSION BBa_K1954002 VERSION BBa_K1954002.1 FEATURES Location/Qualifiers promoter 7..482 /label=pGAP promoter (for Pichia pastoris) CDS 489..5798 /label=Vitellogenin CDS 5805..6650 /label=GFP ORIGIN 1 agatcttttt tgtagaaatg tcttggtgtc ctcgtccaat caggtagcca tctctgaaat 61 atctggctcc gttgcaactc cgaacgacct gctggcaacg taaaattctc cggggtaaaa 121 cttaaatgtg gagtaatgga accagaaacg tctcttccct tctctctcct tccaccgccc 181 gttaccgtcc ctaggaaatt ttactctgct ggagagcttc ttctacggcc cccttgcagc 241 aatgctcttc ccagcattac gttgcgggta aaacggaggt cgtgtacccg acctagcagc 301 ccagggatgg aaaagtcccg gccgtcgctg gcaataatag cgggcggacg catgtcatga 361 gattattgga aaccaccaga atcgaatata aaaggcgaac acctttccca attttggttt 421 ctcctgaccc aaagacttta aatttaattt atttgtccct atttcaatca attgaacaac 481 taatacaaat gttggcccca ggattcgcct ctgttaagac ctacatgtac aaatatgaag 541 cggttcttat gggcggcctg cctgaagagg gcctggctcg agctggggtt aaaatccggg 601 gcaaagtttt gatcagtgca acaagtgcca acgactacat tctgaagctt gtagaccctc 661 agttgctgga gtacagtggc atctggccca aagatccttt ccatccagcc accaagctca 721 ccacagccct ggctactcag ctctcgacac cgatcaagtt tgagtataca aacggcgttg 781 tcgggagact ggctgcacct cctggggtct ccacaacagt gctgaatatc tacaggggaa 841 tcatcaacct cctgcaactg aatgtaaaga agacacagaa cgtctacgag atgcaagagt 901 ctggagctca tggtgtgtgc aagaccaact atgtgatcag ggaggacgcg agagccgaac 961 gcattcatct gaccaagacc aaggacctga accactgcca ggagaaaatc atgaaggcca 1021 tcggcttgga acacgtagag aaatgccatg attgtgaagc tagaggaaag agcctgaagg 1081 gaactgcttc ctataactac atcatgaagc cagcacccag tggttctctg attatggagg 1141 ctgtcgctag agaggtcatc gagttctcac ctttcaacat tttgaatggc gctgctcaga 1201 tggagtctaa gcaaattctg accttcctgg atatcgagaa cacccctgtg gatcatgcca 1261 gatacaccta tgttcaccgc ggatccctgc aatatgagca tggcagcgag attctccaga 1321 cacccatcca tcttctgagg gtcacccatg ccgaggctca gattgtcagc actctgaacc 1381 acctggtagc ctccaacgtg gccaaggtcc atgaagatgc ccctctgaag tttgttgagc 1441 tcatccaggt gatgcgtgtg gccagatttg agactattga gtccctctgg gctcagttta 1501 aatccagacc tgatcacagg tactggttac tgaatgctgt cccccacatt cgcactcacg 1561 ctgcgcttaa gttcctcatt gagaagctcc ttgctaatga gttaagtgag actgaagctg 1621 ctatggctct cttggaatgt ctgcactctg tgacagctga ccagaaaacc attgaacttg 1681 tcagaagcct ggctgagaac cacagagtga aacgtaacgc tgtgctcaac gagattgtga 1741 tgctgggctg gggcactgta atttccaggt tctgtaaagc gcagccatct tgctcatctg 1801 atcttgtgac acctgtacat agacaagttg cagaggctgt tgaaactggt gacatcgatc 1861 agctcactgt cacgctcaaa tgcctggata acgctggaca tcctgctagc attaagacaa 1921 tcatgaagtt cctgcctggc tttggcagtg ctgctgcccg agtcccactc aaagttcagg 1981 ttgacgctgt tctagccctg aggagaattg caaagaggga acccaagatg gtccaggaaa 2041 tagctgctca gttgctcatg gaaaagcatc tccatgcaga actgcgtatg gttgctgcca 2101 tggtgctctt tgagactaaa ctccccgtgg gtctagcagc tagcatttcc acagccttga 2161 tcaaagaaaa gaacctgcaa gtcgttagct ttgtctactc ttacatgaag gccatggcca 2221 agaccacatc ccctgaccac gtttctgttg ctgccgcatg taatgttgcc ttgaggttcc 2281 tcaaccccaa attaggcaga ctgaacttcc gctacagccg agccttccat gtggatacct 2341 ataacaatgc ctggatgatg ggtgctgccg ccagtgccgt cttaattaac gacgctgcaa 2401 ccgtgttacc aagaatgatt atggccaaag cccgtactta catggccgga gcttatgttg 2461 atgcttttga ggttggagtg aggactgagg gaatccagga ggctcttttg aaaagacgac 2521 atgaaaattc tgagaatgca gacaggatca ccaagattaa acaagccatg agagctcttt 2581 ctgagtggag ggctaatcct tcgagccagg ccctggcctc tatgtatgtg aaggtcttcg 2641 gacaagaaat tgcatttgcc aacattgaca aatccaaggt tgaccagctt atccagtttg 2701 ccagtggacc tttgagaaac gtattcagag atgctgtgaa ctctgtgctg tctggttatg 2761 caacacattt tgctaaacca atgctgctcg gtgagctccg tctcatcctt cccaccactg 2821 ttgggttgcc catggagatc agcctcatta catccgctgt gactgctgca tctgttgacg 2881 tccaagccac tgtgtcacca cctctgcctg tcaactaccg agtttcccag cttctggagt 2941 ccgatatcca actgagggct acagttgctc caagtcttgc catgcagacc tatgcattca 3001 tgggtgtgaa caccgcctta atccaggctg ccgtgatgac aaaagccaaa gtttacacag 3061 ctgttcctgc acagataaaa gcaaggattg acattgttaa gggcaacttg aaggttgagt 3121 tcctgtctct ccagggcatt aacacaattg catctgcaca tgcggagacg gttgccattg 3181 caagaaatgt ggaagacctc ccagccgcaa gaagcacacc actgatctca tctgaaactg 3241 catcacaact ttcaaaggcc tctctcaact caaagatctc caggatggca tcctctgtga 3301 ctggtggcat gtctgcgtca tctgaaatca ttcctgctga cctgccaagt aagattggga 3361 ggaaaatgaa actccctaaa acctacagga agaaaatccg tgcttcaagc agaatgctag 3421 gattcaaggc ctacgctgag attgaatctc acaatgccgc ctacatcaga gactgccctc 3481 tctacgctct gatcggaaag catgctgctt ctgttaggat tgctccagct tctggaccag 3541 tcattgagaa gattgaagtt gagattcagg tcggagataa agcagcagaa aatatgatta 3601 aagcgattga catgagcgaa gaggaggaag ctcttgagga taagaatgtc ctcttgaaaa 3661 tcaagaaaat actggcacct ggtctcaaga acaccacatc atcttcctcc agctcctcca 3721 gctcctcttc atccagctct agctccaaca agtcttcttc atccagttcc cgctccagca 3781 gctcccagtc atccagctct cgttcccata ggtctcgctc cagaaagtcc cagtctagca 3841 gctctcagtc aagccgctct ccctcaagct cttcctcctc ttcctcctct tcatcatcca 3901 gatcttcttc caggtcatct tccagatcat cttccagatc ttcttctagg tcctcctctc 3961 gctccagaac taagatggct gacattgttg ctcctattat cacgacgtcc accagagtga 4021 gcagttcctc cagtcgatca gcctctaaca gctcctccag cagtgcttca tacttgctca 4081 gctcatctaa aagaagaagc agaagcagaa gcagcagcag cagtagcagc agcagcagca 4141 gcagcagcag tagcagcagc agcagcagca gcagtagcaa gaacagcaag cgcagcaaga 4201 gcagcaacag caagtcatca agctctaggt cctctcggcg cagtgctcag tctaagcaac 4261 aactgcttgc cttgaagttc agaaagaacc acgtccacag gcatgccatc tccacacagc 4321 gcggcagcag tcacagcagt gcccgcagct tcgattccat ctacaataag gccaagtacc 4381 tcgctaacac actcactcct gccatgtcca ttgcaatccg tgccgtgaga gtcgaccaca 4441 aggtccaggg ataccagcta gcagcttacc tggacaaaca gaccaataga ctgcaattga 4501 tttttgccag agtcgctgag aaggacaact ggagaatctg tgccgacatt gtgcagctga 4561 gttcgcacaa gctgatggcc aagactgcct ggggtgctga atgcaagcaa tactccacca 4621 tgattgtagc tgaaactggt cttttgggtc atgagcccgc agcccgcttg aagctgacct 4681 gggacaaact gccaggaagc ataaagcact acgcaaagag ggcgttgaaa tccattgtcc 4741 ctattgctca agaatatgga gtaaactacg caaaggccaa gaatcctcgt aatcaaatca 4801 aactgactgt agctgttgct actgagacaa gcatgaatat tgtgctgaac acaccaaagg 4861 caatcgttta caagcgtggg gtgtgtctac ctgttgcttt accaattgga aacactgctg 4921 ccgagctgca agcgacccgg gacaactggg ctgacaagat gtcctatttg gttaccaaag 4981 ctaacgcagt tgaatgctcc ctcatcaaca acacactgac cacattcaac aacaggaaag 5041 ctagagatga gctgccacac tcgtgctacc aggtcttggc tcaggattgc acaccagaac 5101 tcaaattcat ggttctgctg aagaaagacc aaatacagga tcagaaccag atcaatgtta 5161 agatttcaga catcgatgtg gacatgtatc ggaagaacaa cgccattgcg gtgatggtta 5221 acggagttga aatccctaac agcaacctgc catacctgca tccatcaggt aacatacata 5281 taagacagtc aaatgaaggc attactctca atgcacccag ccatggtctt caggaggtct 5341 tccttggctt caacgagctg agggttaaag ttgcagactg gatgaaagga aagacttgtg 5401 gtgcctgtgg aacggcaagc ggaaatgtcg gagacgagta ccgcacaccc agtgaacagg 5461 tgaccaagga tgccatcagc tacgcccact cctgggttct gtcttcaaac acctgccgtg 5521 atccctccga gtgttccatc aagcaggaat ctgtgaagct ggagaagcgg gtgatctttg 5581 aaggtgtgga gtccaaatgc tactctgttg agcccgtgct gcaatgcctg cccggctgta 5641 tcccagtgag aaccactacc gtcaacgttg gctttcactg cctgcccagt gacacaactg 5701 tggaccgttc tggtctgagc agcttctttg agaagagcat cgacctgagg gatactgccg 5761 aagcccacct ggcctgtcgc tgcactcctc agtgtgctga gttcatggtt tctaaaggtg 5821 aagaattgtt cactggtgtt gttccaattt tggttgaatt ggatggtgat gttaatggtc 5881 ataaattctc tgtttctggt gaaggtgaag gtgatgctac ttatggtaaa ttgactttga 5941 aattcatttg tactactggt aaattgccag ttccatggcc aactttggtt actactttga 6001 cttatggtgt tcaatgtttc tccagatatc cagatcatat gaaacaacat gatttcttca 6061 aatctgctat gccagaaggt tatgttcaag aaagaactat tttcttcaaa gatgatggta 6121 attataaaac tagagctgaa gttaaattcg aaggtgatac tttggttaat agaattgaat 6181 tgaaaggtat tgatttcaaa gaagatggta atattttggg tcataaattg gaatataatt 6241 ataattctca taatgtttat attatggctg ataaacaaaa aaatggtatt aaagttaatt 6301 tcaaaattag acataatatt gaagatggtt ctgttcaatt ggctgatcat tatcaacaaa 6361 atactccaat tggtgatggt ccagttttgt tgccagataa tcattatttg tctactcaat 6421 ctgctttgtc taaagatcca aatgaaaaaa gagatcatat ggttttgttg gagttcgtta 6481 ctgctgctgg tattactttg ggtatggatg aattgtataa atctggtttg aggtccagag 6541 ctcaagcttc taattctgct gttgatggta ctgctggtcc aggttctact ggttccagag 6601 cttgtaaaaa ttggttctct tctttgtctc atttcgttat tcatttgtaa //