LOCUS BBa_K1954004 9897 bp DNA linear 15-Jun-2024 DEFINITION Mutacin III biosynthetic device ACCESSION BBa_K1954004 VERSION BBa_K1954004.1 FEATURES Location/Qualifiers promoter 1..38 /label=BBa_I14033 promoter 4..9 /label=-35 promoter 27..32 /label=-10 misc_feature 47..58 /label=BBa_B0034 CDS 59..913 /label=mutR misc_feature 67..85 /label=TetR 1 promoter 86..91 /label=-35 misc_feature 92..110 /label=TetR 2 promoter 109..114 /label=-10 misc_feature 129..1338 /label=BBa_I0500 misc_feature 129..1207 /label=AraC misc_feature 958..1029 /label=rrnB T1 misc_feature 1121..1148 /label=rrnB T2 promoter 1179..1197 /label=T7 promoter promoter 1208..1338 /label=Pbad RBS 1220..1231 /label=BBa_B0034 CDS 1238..1429 /label=mutA misc_feature 1507..1554 /label=T7 terminator RBS 1638..1649 /label=BBa_B0034 CDS 1656..4626 /label=muB CDS 4615..5889 /label=mutC CDS 5913..6479 /label=mutD CDS 6484..7827 /label=mutP misc_feature 7872..7943 /label=rrnB T1 promoter 8022..8187 /label=BBa_I0500 RBS 8205..8216 /label=BBa_B0034 CDS 8223..9848 /label=mutT misc_feature 9864..9897 /label=BBa_B1002 ORIGIN 1 ggcacgtaag aggttccaac tttcaccata atgaaacaag aaagaggaga aaatacaaat 61 gaaggtaaac caatcaatgg agctgggtga actgtaccgt gaactgcgta tcgctcgtgg 121 tttgaagatc aaggatatcg cttgtaagaa tctgtccaag tcacaactct ctcgttttga 181 aaatggacaa accatgttgg cagctgataa attgctatta gctatttcgg gcattcatat 241 gagcttttcg gaatttggat atgctttgag ccattatgag gagagtgatt tcttcaagcg 301 cggtcataag ttatcagaat tatatgccca gaaggatatc aagggattaa agaagctgct 361 ggagttcaac gacaaccatg aggtatttga tgtctacaat cgtctgaaca agctggttat 421 tcaagttact attcatttgc tagatactga ttacataata tcagatgatg ataagaattt 481 cctgacaact tacctgtaca atatcgaaga gtggactgag tatgaactgt acatcttcgg 541 gaatactatg tctatattgt catctgatga tctgatcttc ttaggcaagg cttttgtaga 601 acgtgataag ttgtatatat ctcttcctag tcataagaag aatgcagagt taaccttcct 661 gaacttaatc ctgattctgc ttgaacgtaa gaagctgtat caggcaatct acttcgtaga 721 gaatctggag aagctgctga attaccagga tatgttcgca ataacattcc tgaagttcct 781 gaagaagatc atcacttact tccatgataa gtcagtagat atgtctgaac tggagcatta 841 catcaacatc gttgaggaga tcaaccctac gatcgcttca atcctgaagt ctaatctgaa 901 ccagctgctg taataacact gatagtgcta gtgtaaatca ctactagagc caggcatcaa 961 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 1021 aacgctctcc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg 1081 gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat 1141 ggcctttttg cgtattgggc gctcttcttt ataactgata atacgactca ctatagggga 1201 tgtcaaagaa tacaagcata aagaggagaa aatacaaatg tcaaacacac aattattaga 1261 agtccttggt actgaaactt ttgatgttca agaagatctc tttgcttttg atacaacaga 1321 tactactatt gtggcaagca acgacgatcc agatactcgt ttcaaaagtt ggagcctttg 1381 tacgcctggt tgtgcaagga caggtagttt caatagttac tgttgttaat gaaagctaag 1441 cttgatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag 1501 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaca 1561 ggagcaacta tatccggatc tggcgtaata tccctatcag tgatagagat tgacatccct 1621 atcagtgata gagatacaaa gaggagaaaa tactaatgaa cgatttccaa ttccaagatt 1681 acttcatgta ccgcaagcca ctgggcaact tctctaactt cttcagtatc actgatacga 1741 tggatcccat tgagttacta catagtgatc cgatatttgc tgaaggagta tatttggcct 1801 cttcatctct tagagcagcc ataaataaac ttaagaatca tactgcgagt actaaggata 1861 aaaagaatgc aagagagact atttttcaat actatgcccg ttataacacg agatcaactc 1921 cgtttggctt gttttcgtcc atcggagtag gtgctttctc ggcttacctg aagaaggaaa 1981 agtctcgtta cgaaaagtct atcaacatcg atctgttctg ggcttacaag gtagcagata 2041 agctggaaag catgcctgaa atcctgaaca ctctgaaggt agttgctaac aatgctctgc 2101 aaaagtcaga taacttctgg ctgttggata cgcgcagtca tttcggtctg atgaactcac 2161 gttctgatat ccatgaggac atcacagtta agtctaacca gctgatcgat tacgttatca 2221 attgtgcaga acagccaatc tgttacccaa cactggttga taatatcgcc gagaagttct 2281 ctcagtctag tgatggtgta aagaaatact tacagaagct gatcaaggaa gagttcttga 2341 tcactgagct gaagttcagt ctgatcgaag acaaccctct ggattggttc atcaacatcc 2401 tggaacgcac tcagaacaac ctggaactgc ttgaaaagct gactgagata aaggcaatga 2461 ttcaggacta tactgactgt gacattggtg agggcaacaa tttgatttta gctttagaaa 2521 ataagatgag ccaaatagca gaaactaatg catatttgcg ggttgatctt tatgatcatg 2581 cagagttgga gttatctcat catatcaagg attctcttca gaacatcctg aaggtactga 2641 gcagcttctc accaggtatt aatagtcgga aagaaattag aaattaccat gagaaattta 2701 tttccagata tggatatgaa cagttagtac ctcttcaatt acttttgaac tctactagcg 2761 gacttggctt tccaaaaggg tatagtcaga caaaggattt caagcagaat aatggagata 2821 gtcggaacca gaagatcaca gagttcctcc agagaaagtt cgagaagtct ctgagagatg 2881 gtaaggaaat catcctgaat gatgatgatt taaaagatct ggattttgat aaggaacagc 2941 agatatcagg ggaactatac tgtttctacg atttcaagaa ccgcaagctg gaggtgagcg 3001 gcctgggcgt gtcaccgatg atcggagata ccttcggacg tttccatgct aaattgccga 3061 acacgatcat cacagagaac gtaaacaaga cgaaggagat cttcgctaag gcctacccta 3121 acactatcat cactcagctg aacgaagtgc catacttcgg gcgcagcggt aacgttatgg 3181 tcagcaacag cctgaagagc catcagctgg agctgcgcaa ctatactagc aagaaggata 3241 tgagcatcaa tgatatctac gtaggtgcaa ccagcgagga actgtacttc tacagcaaga 3301 agtacggaaa gcgcgttatc ttcatcatga acaacatgtt caactacatc aacggtagca 3361 aactgctgcg tttcctgctg gaagttagca atagcgactt tcaaaatatt actccgatta 3421 tgtttgataa tctggattct tacaaccatg tgcctactat catctataag gacatcatca 3481 tcaagccaga aacatggaat atccgcaaga gcgaagttaa gactctggac agcctcaaga 3541 actggctgag cgataacgat gtgccgagcc tggtgcgcat gaagtacacc gatcagatca 3601 tctacctgga cctgagccgc accatcgacc tgaccatgct gttccgcagc atcaagaagc 3661 acagcttcat ccagctgctg gctacccaca gcgaatttgt gaacgacacg aagatcctgg 3721 agctggtggt gcctttcacc aggagcgatg tcaacgctcg ccagatctac cactacgccc 3781 agaacatcta caccctggag gatagcgata gcaaggagaa gtacttctac gtgaagatct 3841 acgtgaacaa gcagcgccag accagcttcc tgcaaaagga gtacccgctg ctgctgaact 3901 acctgaagct gccggaaaac ctgcaatggt tctacatcag atacgaagat gatgagaagg 3961 acagcatccg cctgcgcatc cgctacgtag aagataagca actggttcaa ctgtacagcc 4021 gcttcatcga gtgggcgaca gaagcacgca agaacataca gatcctgggt tacgaaatca 4081 gcgaatacat cccggaaagc gcacgctacg gtggcaagaa gtacagcaca atcatccaca 4141 gcttcttcta ctacgatagc atcctgagcc tgctgctgca aaagaagaca gatcaggcta 4201 tcgaagtgcg cactagcctg agcatcatcc gcatgttcct gatgatgaag ctgaacctgc 4261 aagatcaaca ggaactgatg aagaacctgt tcgatggaga acacaagctg aagtacgaaa 4321 aggaatacca taacagcatc agcctgttac tggatagcct gtgtacccgc aaccagatcg 4381 atgaagctga taccttctgt gtaatgaaca tgaagatcat cactgaagaa gtgagcagcg 4441 tgctgaagca ggaggactta acaacagatt ggcagagaat cctgggaagt ctgatccaca 4501 tgcgatgtaa tcgagtatac ggcatcaaca gcgagctgga gcgcaagacc atgttcatcg 4561 ttgacaaggt tgttaacagc aagcgctaca cagatatgtt cctggaggtg gataatgaga 4621 caaagtaaac gtgtcgagaa gatcaagaac atcctgaccg agcagaccta cctgttcgac 4681 taccaggagg tgctgaagaa gatcagtcaa gtaaagcaaa cagatttctg gaatctgctg 4741 agcctgagct cgggaatcac cagcctgtta atcttctatc aggagtatga gaatctggaa 4801 ggagtgaacc tgaagccgca aaagcagtca ttgatcggac ttataagtta ctacatcaac 4861 cagatagcag ataagtcctc tctgttcgat ggtctggctg gggtaggttt cgctattaac 4921 tacgtcagca ataacggtaa gtactatcag aagcttctta accagattga cagcaagatc 4981 tgtcagaacg ttaagcggaa ccttgctgat tgtaatagtg aagagtatac aaatcctatg 5041 aattatgatg taatcagcgg aaatgctgga gtagctcgtt atctgatgga acgcgaatcc 5101 tctgaagatt ggcgaattgt tgaaatgatc ttagaagtgt tctacaaggc tttggaacaa 5161 ggctggcgag tgcaaccaaa gtatcaattc ttagaatctg agaagcaata ctacctgaag 5221 ggaaatataa actttggtct ggctcatgga ttattaggtc ctacaacaat tatgtctctg 5281 tatcagcaga ggtacccaca gaacacacgc aatgctgaga agcttcagga gacttaccat 5341 ttcatcaaga gatatgctca actaagagat gaggggctac gatggccaat acgatatgat 5401 ctgcttcgta aggagggtag cttcatcctg cgcaacggct ggtgttacgg agataacggc 5461 atctacagca ccctgttcct gatgggcaag gtgctgagca accagaagat ctgtgagact 5521 gctcagaagg ttataccaag catcatcaag gatgattacg agaagatgga gagcccaaca 5581 ttctgtcatg ggttcgctgg caaggcaaac ttcttcctgc tgcaatatca gagaactaag 5641 gaatcaatct tcctggtgaa ggcagaggaa gaaatcgata agatcctgat cgtgtacaac 5701 agcgagaaca tgttcggatt caaggatatc gaggataaca tcgataacac tgaagagaga 5761 ttaacttatt gggataactt cggtcttctt agtggaactg ttggtgttct attagtgttg 5821 atggaatact gtaacatcgt gaatgctgga aaggtgtcag agtggaataa gatcttcctg 5881 ctgacttaat tattaaagag gagaaaatac aaatggagga acagaataca gagaagaaga 5941 ttctgttgtg tctaacaggc tctggagcct tgttagggat aactgaatat attacgttct 6001 tgactgtgcg cttcaagcat gttcgagtta tcgtctctga taatgctgcg aagatgcttc 6061 ccgttgctgc tattacacaa ttgtgtgaga aagtgtatac tgatgaagtg tcctttacag 6121 ataagcagaa gaatcacata gctttaactc gctgggcaga cataacagtt gtcttacctg 6181 ctacagcaaa catcatcgga aaggttgcta atggtattgc agataacttc atgacaacaa 6241 ctctgcttag ctctagcaag ccagtgttaa tctatccttg catgaacaac atcatgtggg 6301 agaatccagt agttcaaaag aacgttgaag tgctgagcgg aacccaatat aaggtaattg 6361 ttggacaaga atcagaaagc ttcgaattag ccagtggaaa gatgaaaaag aatattgcaa 6421 ttccaagctt ggatgaattg caacgagttg tgttagagaa cttacaagaa gagaggtaag 6481 agtatgaaga agaaaggact gctagtaata atcttcctaa ccttcttctt cttctaccct 6541 aaggctaaag ctgctgagta taccatcatc agcaacaaca gcgagcaaac cgtgaacggc 6601 ctgaacaacc tgggagttac agtcaatagc catattgcgg agattggata tattgaagct 6661 caaggagatg ttaacattga tcagattaag aagctgtcaa acatcaagag tatccagaat 6721 atggctgata catcacagaa tatcacgact agagttcctt caacatatat caaccagaca 6781 atacaattgc ctcagctgtt cagctatcag tgggatatgc agaagattac taataatggt 6841 gtgtcatatt cactgaataa ggagaatcga aagaatgtaa cagttgctct ggttgatagc 6901 gggattgatg tagaccataa tgctttcaca ggaatgattg atagtcgtag caagaacttc 6961 gtgcctgctg gaggatatga taacagtgaa agcagtgaaa ctggaagtat caatgatatt 7021 gatgataaga agggccatgg aacagcagtt gctgggcaaa tcgctgcaaa cggtcaaatc 7081 ttcggtgtgt ccccaggaac gaacctgctt gtctatagag tgttcggcaa gagcaagtca 7141 aaggagtgct ggatcttaaa ggcaatcatc gatgcaacaa acaatggtgc taatgttatt 7201 aatctaagtc tggggcaata catcaagatt cctaatggtg atatctggga gtctgccgaa 7261 gcactgagat acaagttcgc catcgattat gccacaagac ataatgtcat tgttgtagca 7321 gccacaggta atgatggatt aagtgatgac aacggagagg ttaagactta ttacaacagt 7381 cagcattcag gacaagatat gtctcagaat gacacggttg aagattatcc gtctgtgtta 7441 ccgaatgcta ttgcagttgg ctctagcgat aacaacaatc aaagatcaag cttcagtaat 7501 tactacaacc agtaccagga caacttcatc ttggctcctg gtggtggaac accgctgcta 7561 gaccaatatg gtcaagaaga gtggtataat cagaagctgt tcatgaagga acaagtatta 7621 tcaacaagta ataatggaaa ctacgattac gcagatggta ctagcattag cacaggcaag 7681 gtgtctggag agcttgcaga gattattagt aactaccatc ttcaaggaga cagctcaaag 7741 gctagaagta tcctgctgaa ccaggtgaac tacaccagcg atggctacaa ggagatcagc 7801 acttacaagg ctttacgcgg ttactaataa cactgatagt gctagtgtag atcactacta 7861 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 7921 gttgtttgtc ggtgaacgct ctctgcgtct tttactggct cttctcgcta accaaaccgg 7981 taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc 8041 gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca 8101 cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt 8161 tatcgcaact ctctactgtt tctccatacc cgtttttttg ggctaaagag gagaaaatac 8221 aaatgaagtg gttagaagtg ttgcagatta gtaagaagga gaagatcctg taccttatcg 8281 gctgtatctt cagcatcatg acaggcctga tcaccctacg aatcacctac ttacttaaga 8341 atttagtgga cagcaaatcg agcttcaaca acctgttctt gttccttgtg ctgggattag 8401 tgctgttcat catagatgct ggttcacagt atctgatctc attgattggc aatcaagtag 8461 tgttcaacag tcgcaacaac atctggaaga agatctctga ttggacagat agtaaagatg 8521 atagctctga aatggcaggc caccttatta atgatagtga actgatagag aacttcataa 8581 tcagcaccat tcctcaatca atcaacagcg tgatcgttgg aagcggatcc ctggttatgc 8641 tgtttgtgat caacagtaag atgagcctgg aggtgatagg gatctgcttc ctgttattgt 8701 tcattatgca accgttctct cgcatcctga gcaagataag caagagaatc caggaagaca 8761 aggctgaact gatcaacatc gcctcacagt tgagaggaca agtcaagaca atcaagagct 8821 acaacgctca ggattacgcc ttccagaagt tcgatgagca gaaccgccag ctgttccaag 8881 atatcctgaa ccgcataaag atcttcagca tctacagccc gttcctgaac atcctgatcc 8941 tgttcatgat catcatcgtg gtgtggctag gcaacacaga agtacgttca ggaaacctca 9001 ctgtaggttc agcaactatc ttcgtggtgt acatgacaca gctgatcaat ccaattatgc 9061 aactgtcaca actggttgct catatgggga tgcttaatgg cggcgtggaa cgtctgctgg 9121 agtacaacca agctatcccg gagaagaacg gcatcaagaa gatcgatgag atcatcaaca 9181 tcgcgttcga taacgttagc ttcgcttatg ataaccaaga gaacatcatc gagaatgtga 9241 atctgacttt ccagaagggt acttacatct ccatcgttgg tgagagtgga gttgggaaat 9301 caaccttact tgatctgtta gaacacaact acgtaccatc aaagggacga atcttaatca 9361 acggaataga tctggaagaa ttgaacatca agactctgcg caataagata agctatgtat 9421 ctcaagaacc aacaattctg tctgggacaa ttcgtgaact attagacttc aatcagcaac 9481 agcatacaga aactagcctg tggaatgtgc ttgatactgt agaattatca gaactgattc 9541 gcaatttacc gcgaaagtta gattctaagg ttgatgaata tggtggtaac ctctctggag 9601 gtcagatgca acggatctca cttgcaagag gattactgaa agcaggagat gttctgttac 9661 tggatgaaag cttcgccaac atcgatgaag agacctgtct taagataaag ttaaagattg 9721 ctgcttatgc tgaatcacac aagcaaattg tgattgaagt gattcataat ctgaatagaa 9781 tcactcccag tagtatcgtg taccgattgg ctgataagaa gctagaaatc ttgaggagcg 9841 gcttctaata ctagagtcac actcgcaaaa aaccccgctt cggcggggtt ttttcgc //