BBa_K1954006 1 BBa_K1954006 Feedback-resistant N-acetylglutamate synthetase 2016-10-18T11:00:00Z 2016-10-25T07:13:55Z AF008119.1 N-acetylglutamate synthetase (NAGS) catalyzes the first step of the linear biosynthetic pathway of L-arginine as follows: acetyl-coA + L-glutamate = coA + N-acetyl-L-glutamate This sequence is the open reading frame of the feedback resistant mutant PT2M217 NAGS which is not responsive to the inhibition by L-arginine present in the endogenous system. false false _2421_ 32880 32880 9 false One restriction site was removed from the source sequence by silent mutagenesis as follows: PstI 421: Leu 121 (CTG) 46.9 % -> Leu (TTA) 15.2 % false Kamil Żmijewski annotation2530071 1 BBa_B0034 range2530071 1 39 50 annotation2530073 1 BBa_B0014 range2530073 1 1400 1494 annotation2530070 1 BBa_I14033 range2530070 1 1 38 annotation2530072 1 frNAGS range2530072 1 57 1388 BBa_K1954006_sequence 1 ggcacgtaagaggttccaactttcaccataatgaaacaaaagaggagaaaatacaaatggtaaaggaacgtaaaaccgagttggtcgagggattccgccattcggttccctatatcaatacccaccggggaaaaacgtttgtcatcatgctcggcggtgaagccattgagcatgagaatttctccagtatcgttaatgatatcgggttgttgcacaacctcggcatccgtctggtggtggtctatggcgcacgtccgcagatcgacgcaaatctggctgcgcatcaccacgaaccgctgtatcacaagaatatacgtgtgaccgacgccaaaacactggaactggtgaagcaggctgcgggaacattgcaactggatattactgctcgcctgtcgatgagtctcaataacacgccgctgcagggcgcgcatatcaacgtcgtcagtggcaattttattattgcccagccgctgggcgtcgatgacggcgtggattactgccatagcgggcgtatccggcggattgatgaagacgcgatccatcgtcaactggacagcggtgcaatagtgctaatggggccggtcgctgtttcagtcactggcgagagctttaacctgacctcggaagagattgccactcaactggccatcaaactgaaagctgaaaagatgattggtttttgctcttcccagggcgtcactaatgacgacggtgatattgtctccgaacttttccctaacgaagcgcaagcgcgggtagaagcccaggaagagaaaggcgattacaactccggtacggtgcgctttttgcgtggcgcagtgaaagcctgccgcagcggcgtgcgtcgctgtcatttaatcagttatcaggaagatggcgcgctgttgcaagagttgttctcacgcgacggtatcggtacgcagattgtgatggaaagcgccgagcagattcgtcgcgcaacaatcaacgatattggcggtattctggagttgattcgcccactggagcagcaaggtattctggtacgccgttctcgcgagcagctggagatggaaatcgacaaattcaccattattcagcgcgataacacgactattgcctgcgccgcgctctatccgttcccggaagagaagattggggaaatggcctgtgtggcagttcacccggattaccgcagttcatcaaggggtgaagttctgctggaacgcattgccgctcaggcgaagcagagcggcttaagcaaattgtttgtgctgaccacgcgcagtattcactggttccaggaacgtggatttaccccagtggatattgatttactgcccgagagcaaaaagcagttgtacaactaccggcgtaaatccaaagtgttgatggcggatttagggtaataagtttcttctcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttgtttcttc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z