LOCUS BBa_K1954007 5482 bp DNA linear 26-Nov-2024 DEFINITION Green Light Inducible bacteriocin Device (GLID) ACCESSION BBa_K1954007 VERSION BBa_K1954007.1 FEATURES Location/Qualifiers promoter 1..38 /label=BBa_I14033 CDS 57..935 /label=araC RBS 39..50 /label=BBa_B0034 misc_feature 1009..1080 /label=rrnB T1 RBS 1205..1217 /label=BBa_B0032 promoter 1144..1204 /label=BBa_K864400 misc_feature 3494..3534 /label=BBa_B0012 CDS 1224..3484 /label=CcaS promoter 3573..3702 /label=BBa_K206000 RBS 3703..3717 /label=BBa_B0030 CDS 3724..4427 /label=CcaR misc_feature 4435..4468 /label=BBa_B1004 promoter 4507..4744 /label=BBa_K566009 RBS 4745..4764 /label=Improved RBS CDS 4765..5433 /label=BBa_K592009 misc_feature 5442..5482 /label=BBa_B0012 ORIGIN 1 ggcacgtaag aggttccaac tttcaccata atgaaacaaa agaggagaaa atacaaatgg 61 ctgaagcgca aaatgatccc ctgctgccgg gatactcgtt taacgcccat ctggtggcgg 121 gtttaacgcc gattgaggcc aatggttatc tcgatttttt tatcgaccga ccgctgggaa 181 tgaaaggtta tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac 241 gagaatttgt ctgccgaccg ggtgatattt tgctgttccc gccaggagag attcatcact 301 acggtcgtca tccggaggct cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg 361 cctactggca tgaatggctt aactggccgt caatatttgc caatacgggt ttctttcgcc 421 cggatgaagc gcaccagccg catttcagcg acctgtttgg gcaaatcatt aacgccgggc 481 aaggggaagg gcgctattcg gagctgctgg cgataaatct gcttgagcaa ttgttactgc 541 ggcgcatgga agcgattaac gagtcgctcc atccaccgat ggataatcgg gtacgcgagg 601 cttgtcagta catcagcgat cacctggcag acagcaattt tgatatcgcc agcgtcgcac 661 agcatgtttg cctgtcgccg tcgcgtctgt cacatctttt ccgccagcag ttagggatta 721 gcgtcttaag ctggcgcgag gaccaacgca tcagccaggc gaagctgctt ttgagcacta 781 cccggatgcc tatcgccacc gtcggtcgca atgttggttt tgacgatcaa ctctatttct 841 cgcgagtatt taaaaaatgc accggggcca gcccgagcga gttccgtgcc ggttgtgaag 901 aaaaagtgaa tgatgtagcc gtcaagttgt cataataagc tagagacctg gctgttagat 961 cactcagtgg aacgaaaact cacgttaagg gattttggtc atgagattca aataaaacga 1021 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 1081 gttacaagga atagtttcga accacggtta ctaatcctaa taacggaacg ctgtctattc 1141 ttcgagctgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg agcggataac 1201 aatttcacac aggaaagata caaatgggca aatttctaat tccaatcgaa tttgtttttc 1261 tggcgatcgc catgacctgt tatttatggc acagacaaaa ccaagaacgc cgcaggattg 1321 aaattagcat caagcaacaa acccaacggg aacgatttat taaccaaatt acccaacata 1381 tccgccaatc tttaaacttg gaaacggttt taaataccac cgtcgctgaa gttaaaaccc 1441 tgttgcaagt tgatcgagtt ctaatttatc gcatttggca agatggcacg ggcagcgcca 1501 ttacggaatc ggtgaatgcc aattatccta gtattttagg gcggaccttt tccgatgaag 1561 tttttcccgt tgaataccat caagcctaca ccaaaggtaa agtacgggcc attaatgaca 1621 ttgaccagga tgacatagag atttgcctag ctgatttcgt caaacaattt ggcgtgaaat 1681 caaaattagt agtgcccatt cttcaacata atcgtgcttc ttccctagat aatgaatcag 1741 aatttcccta tctttggggg ctgttaatta cccatcaatg tgcttttacc cggccatggc 1801 aaccgtggga agtggagtta atgaaacagc tagccaatca ggtcgcgatc gccatccaac 1861 aatcggaatt atatgagcaa ttacagcaac tcaataaaga tttggaaaac cgagtcgaaa 1921 aacgcaccca gcaacttgcc gccaccaatc aatccctaag aatggaaatc agtgagcgac 1981 aaaaaacgga agccgctctc cgccacacta accatactct gcaatccctg attgcggcct 2041 cccccagggg tatttttacc cttaatttag cagaccaaat tcagatttgg aatcctacag 2101 cagaacgtat ttttggttgg acagaaacag aaattattgc ccatccagaa ttattaacat 2161 ccaacatttt gctggaagat tatcagcaat ttaaacagaa agttttatca ggcatggttt 2221 cccctagcct agaactgaaa tgccagaaga aagatggtag ttggattgaa attgtccttt 2281 ccgctgctcc cctattggat agtgaagaaa atattgccgg attggtggcg gttgtcgccg 2341 atattaccga gcaaaagcgg caggcagaac aaattcgttt gctacaatcc gttgtggtta 2401 atactaatga tgcggtggtg attacggaag cggagcccat tgatgatccc gggccgcgga 2461 ttctctatgt caatgaagca tttactaaaa tcaccggtta tactgctgaa gaaatgctag 2521 gcaaaacccc ccgagtttta cagggaccaa aaaccagtcg cactgaatta gatagggtgc 2581 ggcaagccat tagtcaatgg caatcagtta ccgttgaagt gattaattat cgtaaggatg 2641 gcagtgagtt ttgggtggaa tttagtctgg tgcccgttgc caataaaaca ggtttttaca 2701 cccattggat tgctgtgcaa agggatgtca ctgagcgccg acgcacggag gaagtccgcc 2761 tagctttaga acgggaaaaa gaattaagcc gcctaaaaac tcgttttttc tccatggctt 2821 cccatgaatt tcgtactccc ctcagtacgg ccttagctgc tgcccaatta ctggaaaatt 2881 ctgaagtggc ctggcttgat cccgataagc gtagccggaa cttacaccgt attcaaaatt 2941 ccgtgaaaaa tatggtacag ctcctggatg atattttaat cattaaccgt gccgaagcgg 3001 gcaaattgga gtttaacccg aactggctgg atctgaagct gttgttccag cagttcatcg 3061 aagaaatcca gctgagcgtc agcgaccagt actatttcga cttcatctgt agcgctcagg 3121 atacgaaggc attggtggat gaaaggttag tgcggtctat tttatctaat ctgttatcta 3181 atgcgattaa atactctccc gggggagggc agattaaaat tgccctaagc ctagattcgg 3241 aacagattat ttttgaagtc accgaccagg gcattggcat ttcgccagag gaccaaaagc 3301 aaatttttga accctttcat cggggcaaaa atgtcagaaa tattacggga acaggactcg 3361 gtttaatggt tgccaagaaa tgtgttgact tacacagtgg cagtatcttg ctaaaaagtg 3421 cagttgacca gggaacaaca gttactatct gtttaaaacg ctataaccat ttgcctcgag 3481 cttaagtttc ttctcacact ggctcacctt cgggtgggcc tttctgcgtt tatagtttag 3541 ttgaaaaaat ctaatattcc aatcggctct tcacattgat tatttgcacg gcgtcacact 3601 ttgctatgcc atagcaagat agtccataag attagcggat cctacctgac gctttttatc 3661 gcaactctct actgtttctc cataccgttt ttttgggcta gcattaaaga ggagaaaata 3721 caaatgcgca ttcttttagt ggaggatgac ttgccgctgg cggaaaccct tgctgaagca 3781 ttgagtgacc agctttacac cgttgatatt gccaccgacg cttccctcgc ctgggactat 3841 gcctcccgac tggaatatga cctcgttatt ttggatgtga tgctgccgga gttggacggg 3901 attaccctct gtcaaaaatg gcgatcgcac agttatttaa tgccaatttt gatgatgaca 3961 gccagggata cgatcaatga taaaatcacg ggcttggatg cgggggcgga tgattatgtg 4021 gtcaagccag tggatttggg ggagttattt gccagggtgc gagctttgtt gcgtcggggt 4081 tgtgcaacgt gccaaccagt tttagagtgg gggccaatca ggttggatcc aagcacctat 4141 gaagttagtt atgacaatga ggttttgtct ttgacccgca aggaatacag cattctggaa 4201 ttactactcc gcaatggccg tcgggtgcta agtcggagca tgattatcga tagtatctgg 4261 aagttggaga gtcccccaga ggaagatacg gttaaggtgc atgtgcggag tttgcgacaa 4321 aaattaaaaa gtgccggttt atcagcagat gccattgaaa cggtccatgg cattgggtat 4381 cgtctggcca atttaacgga aaaatctttg tgccaaggga aaaattaata ctagcgccga 4441 aaaccccgct tcggcggggt tttgccgcgt ttgcctggta cagcaggagt atcaaacccg 4501 tacttcagcc cattgtgctt ttctctatca acctcagctt acctgaaggg gtgaacaggt 4561 ctgggttaat tcatgttgcg aaatgtaaca gttttagtcg catcagctaa ctttccgatt 4621 tctttacgat tttctccccc ttttcttcaa ttttactttg ttaggatcgc atttttaatg 4681 ccaacacata ccagttattg gctggacatt aaacaacttt taagtttaat tactaacttt 4741 atcttataag taggagataa aaacatgagt gtgatcgcta aacaaatgac ctacaaggtt 4801 tatatgtcag gcacggtcaa tggacactac tttgaggtcg aaggcgatgg aaaaggtaag 4861 ccctacgagg gggagcagac ggtaaagctc actgtcacca agggcggacc tctgccattt 4921 gcttgggata ttttatcacc acagtgtcag tacggaagca taccattcac caagtaccct 4981 gaagacatcc ctgactatgt aaagcagtca ttcccggagg gctatacatg ggagaggatc 5041 atgaactttg aagatggtgc agtgtgtact gtcagcaatg attccagcat ccaaggcaac 5101 tgtttcatct accatgtcaa gttctctggt ttgaactttc ctcccaatgg acctgtcatg 5161 cagaagaaga cacagggctg ggaacccaac actgagcgtc tctttgcacg agatggaatg 5221 ctgctaggaa acaactttat ggctctgaag ttagaaggag gcggtcacta tttgtgtgaa 5281 tttaaaacta cttacaaggc aaagaagcct gtgaagatgc cagggtatca ctatgttgac 5341 cgcaaactgg atgtaaccaa tcacaacaag gattacactt cggttgagca gtgtgaaatt 5401 tccattgcac gcaaacctgt ggtcgcctaa taagtttctt ctcacactgg ctcaccttcg 5461 ggtgggcctt tctgcgttta ta //