BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K195402 1 BBa_K195402 This part is composed of RBS,Lpp-OmpA-linker and F10scFv 2009-10-20T11:00:00Z 2015-05-08T01:11:16Z none The coding sequence can express F10scFv(BBa_K195400) on outer membrane by OmpA(BBa_K103006). false false _292_ 0 4924 9 Not in stock false none false Syue-Liang Lin component2219507 1 BBa_B0034 component2219515 1 BBa_K195401 annotation2219507 1 BBa_B0034 range2219507 1 1 12 annotation2219515 1 BBa_K195401 range2219515 1 21 1324 BBa_K195401 1 BBa_K195401 Lpp-OmpA-linker -F10scFv 2009-10-19T11:00:00Z 2015-05-08T01:11:16Z Biobrick In Fusion BBa_K195400 with BBa_K103006 . Fusion our BBa_K195400 with BBa_K103006, so the F10scFv can be transported to outer membrane of E. coli. false false _292_ 0 4924 9 Not in stock false Scarless fusion by PCR false Syue-Liang Lin component2059301 1 BBa_K195400 component2059300 1 BBa_K103006 annotation2059301 1 BBa_K195400 range2059301 1 465 1304 annotation2059300 1 BBa_K103006 range2059300 1 1 464 BBa_K103006 1 OmpA OmpA outer membrane protein A fused to linker; displays proteins on cell surface 2008-09-13T11:00:00Z 2015-05-08T01:08:46Z One of our basic bricks used to create fusions attached to outer membrane false true _180_ 0 2582 9 In stock true true Michael Lower annotation1990000 1 OmpA range1990000 1 4 438 annotation1989999 1 NdeI range1989999 1 1 7 annotation1990002 1 SacI range1990002 1 459 464 annotation1990001 1 linker range1990001 1 439 464 annotation1993480 1 ATG range1993480 1 4 6 BBa_K195400 1 F10scFv F10scFv 2009-10-19T11:00:00Z 2015-05-08T01:11:16Z synthesized by Mr.gene Our antibody (F10scFv) can bind to the highly conserved region in HA on many subtypes of the Influenza A virus. F10scFv binds to a common epitope that is a highly conserved pocket in the stem region of Hemagglutinin containing the fusion peptide. false false _292_ 0 4924 9 Not in stock false none false Syue-Liang Lin BBa_K103006_sequence 1 catatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K195400_sequence 1 caggttcagctggtacaatctggtgccgaagtgaaaaaaccgggtagcagtgtgaaagtttcctgtacgagtagcgaggtgacctttagcagtttcgccatttcttgggttcgtcaagctcctggacaaggactggagtggctgggtggtattagcccgatgtttggaacgccaaattatgcccagaaattccagggtcgtgtgactattaccgccgatcagtcaacccgtactgcctatatggatctgcgtagcctgcgcagtgaggatacagcagtctattattgtgcccgttcaccgtcctatatttgctcaggtggcacgtgtgtgtttgatcattggggtcagggtactctggttaccgtttcctctggtggtggtggttcaggtggtggtggtagtggtggagggggtattcaaccggggctgacacaacctcctagcgtgtcgaaaggcctgcgtcagacagcaacgctgacgtgtactggcaatagcaacaatgtggggaatcagggagcagcatggctgcaacagcatcagggtcatccgcctaaactgctgtcttatcgtaacaacgatcgtccgagcgggatttcagaacgttttagtgcctcccgtagcgggaacaccgcttcgctgactatcacggggctgcaaccggaagatgaagccgattattattgctcgacctgggattcttctctgagtgccgtggtctttggtgggggaaccaaactgacagtgctgggacagcctaaagcagcaccttcagcggctgccgaacaaaaactgattagcgaggaggatctgaatggagcggcacatcatcaccatcatcat BBa_K195402_sequence 1 aaagaggagaaatactagagcatatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagctccaggttcagctggtacaatctggtgccgaagtgaaaaaaccgggtagcagtgtgaaagtttcctgtacgagtagcgaggtgacctttagcagtttcgccatttcttgggttcgtcaagctcctggacaaggactggagtggctgggtggtattagcccgatgtttggaacgccaaattatgcccagaaattccagggtcgtgtgactattaccgccgatcagtcaacccgtactgcctatatggatctgcgtagcctgcgcagtgaggatacagcagtctattattgtgcccgttcaccgtcctatatttgctcaggtggcacgtgtgtgtttgatcattggggtcagggtactctggttaccgtttcctctggtggtggtggttcaggtggtggtggtagtggtggagggggtattcaaccggggctgacacaacctcctagcgtgtcgaaaggcctgcgtcagacagcaacgctgacgtgtactggcaatagcaacaatgtggggaatcagggagcagcatggctgcaacagcatcagggtcatccgcctaaactgctgtcttatcgtaacaacgatcgtccgagcgggatttcagaacgttttagtgcctcccgtagcgggaacaccgcttcgctgactatcacggggctgcaaccggaagatgaagccgattattattgctcgacctgggattcttctctgagtgccgtggtctttggtgggggaaccaaactgacagtgctgggacagcctaaagcagcaccttcagcggctgccgaacaaaaactgattagcgaggaggatctgaatggagcggcacatcatcaccatcatcat BBa_K195401_sequence 1 catatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagctccaggttcagctggtacaatctggtgccgaagtgaaaaaaccgggtagcagtgtgaaagtttcctgtacgagtagcgaggtgacctttagcagtttcgccatttcttgggttcgtcaagctcctggacaaggactggagtggctgggtggtattagcccgatgtttggaacgccaaattatgcccagaaattccagggtcgtgtgactattaccgccgatcagtcaacccgtactgcctatatggatctgcgtagcctgcgcagtgaggatacagcagtctattattgtgcccgttcaccgtcctatatttgctcaggtggcacgtgtgtgtttgatcattggggtcagggtactctggttaccgtttcctctggtggtggtggttcaggtggtggtggtagtggtggagggggtattcaaccggggctgacacaacctcctagcgtgtcgaaaggcctgcgtcagacagcaacgctgacgtgtactggcaatagcaacaatgtggggaatcagggagcagcatggctgcaacagcatcagggtcatccgcctaaactgctgtcttatcgtaacaacgatcgtccgagcgggatttcagaacgttttagtgcctcccgtagcgggaacaccgcttcgctgactatcacggggctgcaaccggaagatgaagccgattattattgctcgacctgggattcttctctgagtgccgtggtctttggtgggggaaccaaactgacagtgctgggacagcctaaagcagcaccttcagcggctgccgaacaaaaactgattagcgaggaggatctgaatggagcggcacatcatcaccatcatcat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z