LOCUS BBa_K195403 1386 bp DNA linear 22-Dec-2024 DEFINITION This part is composed of pTetR,RBS,Lpp-OmpA-linker and F10scFv ACCESSION BBa_K195403 VERSION BBa_K195403.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 83..89 /label=NdeI CDS 86..520 /label=OmpA CDS 521..546 /label=linker misc_feature 541..546 /label=SacI misc_feature 86..88 /label=ATG misc_feature 83..546 /label=BBa_K103006 CDS 547..1386 /label=BBa_K195400 CDS 83..1386 /label=BBa_K195401 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag agcatatgaa agctactaaa ctggtactgg gcgcggtaat 121 cctgggttct actctgctgg caggttgctc cagcaacgct aaaatcgatc agggaattaa 181 cccgtatgtt ggctttgaaa tgggttacga ctggttaggt cgtatgccgt acaaaggcag 241 cgttgaaaac ggtgcataca aagctcaggg cgttcaactg accgctaaac tgggttaccc 301 aatcactgac gacctggaca tctacactcg tctgggtggc atggtatggc gtgcagacac 361 taaatccaac gtttatggta aaaaccacga caccggcgtt tctccggtct tcgctggcgg 421 tgttgagtac gcgatcactc ctgaaatcgc tacccgtctg gaataccagt ggaccaacaa 481 catcggtgac gcacacacca tcggcactcg tccggacaac ggcggaggtt ctggaggagg 541 gagctccagg ttcagctggt acaatctggt gccgaagtga aaaaaccggg tagcagtgtg 601 aaagtttcct gtacgagtag cgaggtgacc tttagcagtt tcgccatttc ttgggttcgt 661 caagctcctg gacaaggact ggagtggctg ggtggtatta gcccgatgtt tggaacgcca 721 aattatgccc agaaattcca gggtcgtgtg actattaccg ccgatcagtc aacccgtact 781 gcctatatgg atctgcgtag cctgcgcagt gaggatacag cagtctatta ttgtgcccgt 841 tcaccgtcct atatttgctc aggtggcacg tgtgtgtttg atcattgggg tcagggtact 901 ctggttaccg tttcctctgg tggtggtggt tcaggtggtg gtggtagtgg tggagggggt 961 attcaaccgg ggctgacaca acctcctagc gtgtcgaaag gcctgcgtca gacagcaacg 1021 ctgacgtgta ctggcaatag caacaatgtg gggaatcagg gagcagcatg gctgcaacag 1081 catcagggtc atccgcctaa actgctgtct tatcgtaaca acgatcgtcc gagcgggatt 1141 tcagaacgtt ttagtgcctc ccgtagcggg aacaccgctt cgctgactat cacggggctg 1201 caaccggaag atgaagccga ttattattgc tcgacctggg attcttctct gagtgccgtg 1261 gtctttggtg ggggaaccaa actgacagtg ctgggacagc ctaaagcagc accttcagcg 1321 gctgccgaac aaaaactgat tagcgaggag gatctgaatg gagcggcaca tcatcaccat 1381 catcat //