BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986786 1 TetR 2 range1986786 1 26 44 annotation1986785 1 -35 range1986785 1 20 25 annotation1986787 1 -10 range1986787 1 43 48 BBa_K103006 1 OmpA OmpA outer membrane protein A fused to linker; displays proteins on cell surface 2008-09-13T11:00:00Z 2015-05-08T01:08:46Z One of our basic bricks used to create fusions attached to outer membrane false true _180_ 0 2582 9 In stock true true Michael Lower annotation1993480 1 ATG range1993480 1 4 6 annotation1989999 1 NdeI range1989999 1 1 7 annotation1990000 1 OmpA range1990000 1 4 438 annotation1990002 1 SacI range1990002 1 459 464 annotation1990001 1 linker range1990001 1 439 464 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K195403 1 BBa_K195403 This part is composed of pTetR,RBS,Lpp-OmpA-linker and F10scFv 2009-10-20T11:00:00Z 2015-05-08T01:11:16Z none Fusion our BBa_K195400 with BBa_K103006, so our F10scFv can be transported to outer membrane of E. coli. false false _292_ 0 4924 9 Not in stock false none false Syue-Liang Lin component2220567 1 BBa_R0040 component2220573 1 BBa_B0034 component2220581 1 BBa_K195401 annotation2220573 1 BBa_B0034 range2220573 1 63 74 annotation2220581 1 BBa_K195401 range2220581 1 83 1386 annotation2220567 1 BBa_R0040 range2220567 1 1 54 BBa_K195401 1 BBa_K195401 Lpp-OmpA-linker -F10scFv 2009-10-19T11:00:00Z 2015-05-08T01:11:16Z Biobrick In Fusion BBa_K195400 with BBa_K103006 . Fusion our BBa_K195400 with BBa_K103006, so the F10scFv can be transported to outer membrane of E. coli. false false _292_ 0 4924 9 Not in stock false Scarless fusion by PCR false Syue-Liang Lin component2059300 1 BBa_K103006 component2059301 1 BBa_K195400 annotation2059301 1 BBa_K195400 range2059301 1 465 1304 annotation2059300 1 BBa_K103006 range2059300 1 1 464 BBa_K195400 1 F10scFv F10scFv 2009-10-19T11:00:00Z 2015-05-08T01:11:16Z synthesized by Mr.gene Our antibody (F10scFv) can bind to the highly conserved region in HA on many subtypes of the Influenza A virus. F10scFv binds to a common epitope that is a highly conserved pocket in the stem region of Hemagglutinin containing the fusion peptide. false false _292_ 0 4924 9 Not in stock false none false Syue-Liang Lin BBa_K103006_sequence 1 catatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K195403_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagagcatatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagctccaggttcagctggtacaatctggtgccgaagtgaaaaaaccgggtagcagtgtgaaagtttcctgtacgagtagcgaggtgacctttagcagtttcgccatttcttgggttcgtcaagctcctggacaaggactggagtggctgggtggtattagcccgatgtttggaacgccaaattatgcccagaaattccagggtcgtgtgactattaccgccgatcagtcaacccgtactgcctatatggatctgcgtagcctgcgcagtgaggatacagcagtctattattgtgcccgttcaccgtcctatatttgctcaggtggcacgtgtgtgtttgatcattggggtcagggtactctggttaccgtttcctctggtggtggtggttcaggtggtggtggtagtggtggagggggtattcaaccggggctgacacaacctcctagcgtgtcgaaaggcctgcgtcagacagcaacgctgacgtgtactggcaatagcaacaatgtggggaatcagggagcagcatggctgcaacagcatcagggtcatccgcctaaactgctgtcttatcgtaacaacgatcgtccgagcgggatttcagaacgttttagtgcctcccgtagcgggaacaccgcttcgctgactatcacggggctgcaaccggaagatgaagccgattattattgctcgacctgggattcttctctgagtgccgtggtctttggtgggggaaccaaactgacagtgctgggacagcctaaagcagcaccttcagcggctgccgaacaaaaactgattagcgaggaggatctgaatggagcggcacatcatcaccatcatcat BBa_K195400_sequence 1 caggttcagctggtacaatctggtgccgaagtgaaaaaaccgggtagcagtgtgaaagtttcctgtacgagtagcgaggtgacctttagcagtttcgccatttcttgggttcgtcaagctcctggacaaggactggagtggctgggtggtattagcccgatgtttggaacgccaaattatgcccagaaattccagggtcgtgtgactattaccgccgatcagtcaacccgtactgcctatatggatctgcgtagcctgcgcagtgaggatacagcagtctattattgtgcccgttcaccgtcctatatttgctcaggtggcacgtgtgtgtttgatcattggggtcagggtactctggttaccgtttcctctggtggtggtggttcaggtggtggtggtagtggtggagggggtattcaaccggggctgacacaacctcctagcgtgtcgaaaggcctgcgtcagacagcaacgctgacgtgtactggcaatagcaacaatgtggggaatcagggagcagcatggctgcaacagcatcagggtcatccgcctaaactgctgtcttatcgtaacaacgatcgtccgagcgggatttcagaacgttttagtgcctcccgtagcgggaacaccgcttcgctgactatcacggggctgcaaccggaagatgaagccgattattattgctcgacctgggattcttctctgagtgccgtggtctttggtgggggaaccaaactgacagtgctgggacagcctaaagcagcaccttcagcggctgccgaacaaaaactgattagcgaggaggatctgaatggagcggcacatcatcaccatcatcat BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_K195401_sequence 1 catatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagctccaggttcagctggtacaatctggtgccgaagtgaaaaaaccgggtagcagtgtgaaagtttcctgtacgagtagcgaggtgacctttagcagtttcgccatttcttgggttcgtcaagctcctggacaaggactggagtggctgggtggtattagcccgatgtttggaacgccaaattatgcccagaaattccagggtcgtgtgactattaccgccgatcagtcaacccgtactgcctatatggatctgcgtagcctgcgcagtgaggatacagcagtctattattgtgcccgttcaccgtcctatatttgctcaggtggcacgtgtgtgtttgatcattggggtcagggtactctggttaccgtttcctctggtggtggtggttcaggtggtggtggtagtggtggagggggtattcaaccggggctgacacaacctcctagcgtgtcgaaaggcctgcgtcagacagcaacgctgacgtgtactggcaatagcaacaatgtggggaatcagggagcagcatggctgcaacagcatcagggtcatccgcctaaactgctgtcttatcgtaacaacgatcgtccgagcgggatttcagaacgttttagtgcctcccgtagcgggaacaccgcttcgctgactatcacggggctgcaaccggaagatgaagccgattattattgctcgacctgggattcttctctgagtgccgtggtctttggtgggggaaccaaactgacagtgctgggacagcctaaagcagcaccttcagcggctgccgaacaaaaactgattagcgaggaggatctgaatggagcggcacatcatcaccatcatcat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z