LOCUS       BBa_K195404             1523 bp    DNA     linear       01-Nov-2024
DEFINITION  This part is composed of pTetR,RBS,Lpp-OmpA-linker,F10scFv and 
            terminator
ACCESSION   BBa_K195404
VERSION     BBa_K195404.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    83..89
                     /label=NdeI
     CDS             86..520
                     /label=OmpA
     CDS             521..546
                     /label=linker
     misc_feature    541..546
                     /label=SacI
     misc_feature    86..88
                     /label=ATG
     misc_feature    83..546
                     /label=BBa_K103006
     CDS             547..1386
                     /label=BBa_K195400
     CDS             83..1386
                     /label=BBa_K195401
     stem_loop       1406..1449
                     /label=stem_loop
     misc_feature    1395..1474
                     /label=BBa_B0010
     terminator      1395..1474
                     /label=BBa_B0010
     stem_loop       1490..1509
                     /label=T7 TE
     misc_feature    1516..1516
                     /label=stop
     polyA_site      1510..1523
                     /label=polya
     misc_feature    1483..1523
                     /label=BBa_B0012
     terminator      1483..1523
                     /label=BBa_B0012
     terminator      1395..1523
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag agcatatgaa agctactaaa ctggtactgg gcgcggtaat
      121 cctgggttct actctgctgg caggttgctc cagcaacgct aaaatcgatc agggaattaa
      181 cccgtatgtt ggctttgaaa tgggttacga ctggttaggt cgtatgccgt acaaaggcag
      241 cgttgaaaac ggtgcataca aagctcaggg cgttcaactg accgctaaac tgggttaccc
      301 aatcactgac gacctggaca tctacactcg tctgggtggc atggtatggc gtgcagacac
      361 taaatccaac gtttatggta aaaaccacga caccggcgtt tctccggtct tcgctggcgg
      421 tgttgagtac gcgatcactc ctgaaatcgc tacccgtctg gaataccagt ggaccaacaa
      481 catcggtgac gcacacacca tcggcactcg tccggacaac ggcggaggtt ctggaggagg
      541 gagctccagg ttcagctggt acaatctggt gccgaagtga aaaaaccggg tagcagtgtg
      601 aaagtttcct gtacgagtag cgaggtgacc tttagcagtt tcgccatttc ttgggttcgt
      661 caagctcctg gacaaggact ggagtggctg ggtggtatta gcccgatgtt tggaacgcca
      721 aattatgccc agaaattcca gggtcgtgtg actattaccg ccgatcagtc aacccgtact
      781 gcctatatgg atctgcgtag cctgcgcagt gaggatacag cagtctatta ttgtgcccgt
      841 tcaccgtcct atatttgctc aggtggcacg tgtgtgtttg atcattgggg tcagggtact
      901 ctggttaccg tttcctctgg tggtggtggt tcaggtggtg gtggtagtgg tggagggggt
      961 attcaaccgg ggctgacaca acctcctagc gtgtcgaaag gcctgcgtca gacagcaacg
     1021 ctgacgtgta ctggcaatag caacaatgtg gggaatcagg gagcagcatg gctgcaacag
     1081 catcagggtc atccgcctaa actgctgtct tatcgtaaca acgatcgtcc gagcgggatt
     1141 tcagaacgtt ttagtgcctc ccgtagcggg aacaccgctt cgctgactat cacggggctg
     1201 caaccggaag atgaagccga ttattattgc tcgacctggg attcttctct gagtgccgtg
     1261 gtctttggtg ggggaaccaa actgacagtg ctgggacagc ctaaagcagc accttcagcg
     1321 gctgccgaac aaaaactgat tagcgaggag gatctgaatg gagcggcaca tcatcaccat
     1381 catcattact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1441 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1501 gggtgggcct ttctgcgttt ata
//