##gff-version 3
##sequence-region BBa_K195404 1 1523
BBa_K195404	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K195404	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K195404	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K195404	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K195404	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K195404	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K195404	.	CDS	83	1386	.	+	0	ID=BBa_K195401;Name=BBa_K195401
BBa_K195404	.	tag	83	546	.	+	0	ID=BBa_K103006;Name=BBa_K103006;Parent=BBa_K195401
BBa_K195404	.	CDS	86	520	.	+	0	ID=annotation1990000;Name=OmpA;Parent=BBa_K103006
BBa_K195404	.	sequence_feature	541	546	.	+	0	ID=annotation1990002;Name=SacI;Parent=BBa_K103006
BBa_K195404	.	sequence_feature	83	89	.	+	0	ID=annotation1989999;Name=NdeI;Parent=BBa_K103006
BBa_K195404	.	start_codon	86	88	.	+	0	ID=annotation1993480;Name=ATG;Parent=BBa_K103006
BBa_K195404	.	CDS	521	546	.	+	0	ID=annotation1990001;Name=linker;Parent=BBa_K103006
BBa_K195404	.	CDS	547	1386	.	+	0	ID=BBa_K195400;Name=BBa_K195400;Parent=BBa_K195401
BBa_K195404	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K195404	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K195404	.	terminator	1395	1523	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K195404	.	terminator	1483	1523	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K195404	.	engineered_region	1483	1523	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K195404	.	stem_loop	1490	1509	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K195404	.	stop_codon	1516	1516	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K195404	.	polyA_site	1510	1523	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K195404	.	terminator	1395	1474	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K195404	.	engineered_region	1395	1474	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K195404	.	stem_loop	1406	1449	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K195404
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gagcatatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacg
ctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaagg
cagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctg
gacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccg
gcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggac
caacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagctccaggttc
agctggtacaatctggtgccgaagtgaaaaaaccgggtagcagtgtgaaagtttcctgtacgagtagcgaggtgacctt
tagcagtttcgccatttcttgggttcgtcaagctcctggacaaggactggagtggctgggtggtattagcccgatgttt
ggaacgccaaattatgcccagaaattccagggtcgtgtgactattaccgccgatcagtcaacccgtactgcctatatgg
atctgcgtagcctgcgcagtgaggatacagcagtctattattgtgcccgttcaccgtcctatatttgctcaggtggcac
gtgtgtgtttgatcattggggtcagggtactctggttaccgtttcctctggtggtggtggttcaggtggtggtggtagt
ggtggagggggtattcaaccggggctgacacaacctcctagcgtgtcgaaaggcctgcgtcagacagcaacgctgacgt
gtactggcaatagcaacaatgtggggaatcagggagcagcatggctgcaacagcatcagggtcatccgcctaaactgct
gtcttatcgtaacaacgatcgtccgagcgggatttcagaacgttttagtgcctcccgtagcgggaacaccgcttcgctg
actatcacggggctgcaaccggaagatgaagccgattattattgctcgacctgggattcttctctgagtgccgtggtct
ttggtgggggaaccaaactgacagtgctgggacagcctaaagcagcaccttcagcggctgccgaacaaaaactgattag
cgaggaggatctgaatggagcggcacatcatcaccatcatcattactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttata