BBa_K195404
type: ComponentDefinition
persistent identity: BBa K195404
display id: BBa_K195404
version: 1
was generated by: iGEM to SBOL conversion
description: This part is composed of pTetR,RBS,Lpp-OmpA-linker,F10scFv and terminator
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:16Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: Fusion our BBa_K195400 with BBa_K103006, so our F10scFv can be transported to outer membrane of E. coli.
discontinued: false
dominant: false
group u list: _292_
m user id: 0
owner id: 4924
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Syue-Liang Lin
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0040
component: BBa_B0015
component: BBa_K195401
component: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K195401
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K195404_sequence/1

BBa_R0040
type: Component
persistent identity: component2224215
display id: component2224215
version: 1
top level: BBa_K195404
definition: p(tetR)
access: public

BBa_B0015
type: Component
persistent identity: component2224236
display id: component2224236
version: 1
top level: BBa_K195404
definition: BBa_B0015
access: public

BBa_K195401
type: Component
persistent identity: component2224229
display id: component2224229
version: 1
top level: BBa_K195404
definition: BBa_K195401
access: public

BBa_B0034
type: Component
persistent identity: component2224221
display id: component2224221
version: 1
top level: BBa_K195404
definition: BBa_B0034
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2224215
display id: annotation2224215
version: 1
top level: BBa_K195404
location: https://synbiohub.org/public/igem/BBa_K195404/annotation2224215/range2224215/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K195404/annotation2224215/range2224215/1
type: Range
persistent identity: range2224215
display id: range2224215
version: 1
top level: BBa_K195404
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2224221
display id: annotation2224221
version: 1
top level: BBa_K195404
location: https://synbiohub.org/public/igem/BBa_K195404/annotation2224221/range2224221/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K195404/annotation2224221/range2224221/1
type: Range
persistent identity: range2224221
display id: range2224221
version: 1
top level: BBa_K195404
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_K195401
type: SequenceAnnotation
persistent identity: annotation2224229
display id: annotation2224229
version: 1
top level: BBa_K195404
location: https://synbiohub.org/public/igem/BBa_K195404/annotation2224229/range2224229/1
component: BBa_K195401

https://synbiohub.org/public/igem/BBa_K195404/annotation2224229/range2224229/1
type: Range
persistent identity: range2224229
display id: range2224229
version: 1
top level: BBa_K195404
direction: direction/reverse
start: 83
end: 1386
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2224236
display id: annotation2224236
version: 1
top level: BBa_K195404
location: https://synbiohub.org/public/igem/BBa_K195404/annotation2224236/range2224236/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K195404/annotation2224236/range2224236/1
type: Range
persistent identity: range2224236
display id: range2224236
version: 1
top level: BBa_K195404
direction: direction/reverse
start: 1395
end: 1523
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K195401
type: ComponentDefinition
persistent identity: BBa K195401
display id: BBa_K195401
version: 1
was generated by: iGEM to SBOL conversion
description: Lpp-OmpA-linker -F10scFv
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:16Z
mutable provenance: Biobrick
owned by: james
owned by: myers
mutable description: In Fusion BBa_K195400 with BBa_K103006 . Fusion our BBa_K195400 with BBa_K103006, so the F10scFv can be transported to outer membrane of E. coli.
discontinued: false
dominant: false
experience: experience/None
group u list: _292_
m user id: 0
owner id: 4924
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Scarless fusion by PCR
star: false
creator: Syue-Liang Lin
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_K195400
component: BBa_K103006
sequence annotation: BBa_K103006
sequence annotation: BBa_K195400
sequence: https://synbiohub.org/public/igem/BBa_K195401_sequence/1

BBa_K195400
type: Component
persistent identity: component2059301
display id: component2059301
version: 1
top level: BBa_K195401
definition: F10scFv
access: public

BBa_K103006
type: Component
persistent identity: component2059300
display id: component2059300
version: 1
top level: BBa_K195401
definition: OmpA
access: public

BBa_K103006
type: SequenceAnnotation
persistent identity: annotation2059300
display id: annotation2059300
version: 1
top level: BBa_K195401
location: https://synbiohub.org/public/igem/BBa_K195401/annotation2059300/range2059300/1
component: BBa_K103006

https://synbiohub.org/public/igem/BBa_K195401/annotation2059300/range2059300/1
type: Range
persistent identity: range2059300
display id: range2059300
version: 1
top level: BBa_K195401
direction: direction/reverse
start: 1
end: 464
orientation: inline

BBa_K195400
type: SequenceAnnotation
persistent identity: annotation2059301
display id: annotation2059301
version: 1
top level: BBa_K195401
location: https://synbiohub.org/public/igem/BBa_K195401/annotation2059301/range2059301/1
component: BBa_K195400

https://synbiohub.org/public/igem/BBa_K195401/annotation2059301/range2059301/1
type: Range
persistent identity: range2059301
display id: range2059301
version: 1
top level: BBa_K195401
direction: direction/reverse
start: 465
end: 1304
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

OmpA
type: ComponentDefinition
persistent identity: BBa K103006
display id: BBa_K103006
version: 1
was generated by: iGEM to SBOL conversion
description: OmpA outer membrane protein A fused to linker; displays proteins on cell surface
created: 2008-09-13T11:00:00Z
modified: 2015-05-08T01:08:46Z
owned by: james
owned by: myers
mutable description: One of our basic bricks used to create fusions attached to outer membrane
discontinued: false
dominant: true
experience: experience/Works
group u list: _180_
m user id: 0
owner id: 2582
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Michael Lower
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence annotation: NdeI
sequence annotation: ATG
sequence annotation: OmpA
sequence annotation: linker
sequence annotation: SacI
sequence: https://synbiohub.org/public/igem/BBa_K103006_sequence/1

NdeI
type: SequenceAnnotation
persistent identity: annotation1989999
display id: annotation1989999
version: 1
top level: OmpA
location: https://synbiohub.org/public/igem/BBa_K103006/annotation1989999/range1989999/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K103006/annotation1989999/range1989999/1
type: Range
persistent identity: range1989999
display id: range1989999
version: 1
top level: OmpA
direction: direction/forward
start: 1
end: 7
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation1993480
display id: annotation1993480
version: 1
top level: OmpA
location: https://synbiohub.org/public/igem/BBa_K103006/annotation1993480/range1993480/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K103006/annotation1993480/range1993480/1
type: Range
persistent identity: range1993480
display id: range1993480
version: 1
top level: OmpA
direction: direction/forward
start: 4
end: 6
orientation: inline

OmpA
type: SequenceAnnotation
persistent identity: annotation1990000
display id: annotation1990000
version: 1
top level: OmpA
location: https://synbiohub.org/public/igem/BBa_K103006/annotation1990000/range1990000/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K103006/annotation1990000/range1990000/1
type: Range
persistent identity: range1990000
display id: range1990000
version: 1
top level: OmpA
direction: direction/forward
start: 4
end: 438
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1990001
display id: annotation1990001
version: 1
top level: OmpA
location: https://synbiohub.org/public/igem/BBa_K103006/annotation1990001/range1990001/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K103006/annotation1990001/range1990001/1
type: Range
persistent identity: range1990001
display id: range1990001
version: 1
top level: OmpA
direction: direction/forward
start: 439
end: 464
orientation: inline

SacI
type: SequenceAnnotation
persistent identity: annotation1990002
display id: annotation1990002
version: 1
top level: OmpA
location: https://synbiohub.org/public/igem/BBa_K103006/annotation1990002/range1990002/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K103006/annotation1990002/range1990002/1
type: Range
persistent identity: range1990002
display id: range1990002
version: 1
top level: OmpA
direction: direction/forward
start: 459
end: 464
orientation: inline

F10scFv
type: ComponentDefinition
persistent identity: BBa K195400
display id: BBa_K195400
version: 1
was generated by: iGEM to SBOL conversion
description: F10scFv
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:16Z
mutable provenance: synthesized by Mr.gene
owned by: james
owned by: myers
mutable description: Our antibody (F10scFv) can bind to the highly conserved region in HA on many subtypes of the Influenza A virus. F10scFv binds to a common epitope that is a highly conserved pocket in the stem region of Hemagglutinin containing the fusion peptide.
discontinued: false
dominant: false
experience: experience/None
group u list: _292_
m user id: 0
owner id: 4924
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Syue-Liang Lin
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K195400_sequence/1

https://synbiohub.org/public/igem/BBa_K195404_sequence/1
type: Sequence
persistent identity: BBa K195404 sequence
display id: BBa_K195404_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagagcatatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagctccaggttcagctggtacaatctggtgccgaagtgaaaaaaccgggtagcagtgtgaaagtttcctgtacgagtagcgaggtgacctttagcagtttcgccatttcttgggttcgtcaagctcctggacaaggactggagtggctgggtggtattagcccgatgtttggaacgccaaattatgcccagaaattccagggtcgtgtgactattaccgccgatcagtcaacccgtactgcctatatggatctgcgtagcctgcgcagtgaggatacagcagtctattattgtgcccgttcaccgtcctatatttgctcaggtggcacgtgtgtgtttgatcattggggtcagggtactctggttaccgtttcctctggtggtggtggttcaggtggtggtggtagtggtggagggggtattcaaccggggctgacacaacctcctagcgtgtcgaaaggcctgcgtcagacagcaacgctgacgtgtactggcaatagcaacaatgtggggaatcagggagcagcatggctgcaacagcatcagggtcatccgcctaaactgctgtcttatcgtaacaacgatcgtccgagcgggatttcagaacgttttagtgcctcccgtagcgggaacaccgcttcgctgactatcacggggctgcaaccggaagatgaagccgattattattgctcgacctgggattcttctctgagtgccgtggtctttggtgggggaaccaaactgacagtgctgggacagcctaaagcagcaccttcagcggctgccgaacaaaaactgattagcgaggaggatctgaatggagcggcacatcatcaccatcatcattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K195401_sequence/1
type: Sequence
persistent identity: BBa K195401 sequence
display id: BBa_K195401_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagctccaggttcagctggtacaatctggtgccgaagtgaaaaaaccgggtagcagtgtgaaagtttcctgtacgagtagcgaggtgacctttagcagtttcgccatttcttgggttcgtcaagctcctggacaaggactggagtggctgggtggtattagcccgatgtttggaacgccaaattatgcccagaaattccagggtcgtgtgactattaccgccgatcagtcaacccgtactgcctatatggatctgcgtagcctgcgcagtgaggatacagcagtctattattgtgcccgttcaccgtcctatatttgctcaggtggcacgtgtgtgtttgatcattggggtcagggtactctggttaccgtttcctctggtggtggtggttcaggtggtggtggtagtggtggagggggtattcaaccggggctgacacaacctcctagcgtgtcgaaaggcctgcgtcagacagcaacgctgacgtgtactggcaatagcaacaatgtggggaatcagggagcagcatggctgcaacagcatcagggtcatccgcctaaactgctgtcttatcgtaacaacgatcgtccgagcgggatttcagaacgttttagtgcctcccgtagcgggaacaccgcttcgctgactatcacggggctgcaaccggaagatgaagccgattattattgctcgacctgggattcttctctgagtgccgtggtctttggtgggggaaccaaactgacagtgctgggacagcctaaagcagcaccttcagcggctgccgaacaaaaactgattagcgaggaggatctgaatggagcggcacatcatcaccatcatcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K103006_sequence/1
type: Sequence
persistent identity: BBa K103006 sequence
display id: BBa_K103006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K195400_sequence/1
type: Sequence
persistent identity: BBa K195400 sequence
display id: BBa_K195400_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caggttcagctggtacaatctggtgccgaagtgaaaaaaccgggtagcagtgtgaaagtttcctgtacgagtagcgaggtgacctttagcagtttcgccatttcttgggttcgtcaagctcctggacaaggactggagtggctgggtggtattagcccgatgtttggaacgccaaattatgcccagaaattccagggtcgtgtgactattaccgccgatcagtcaacccgtactgcctatatggatctgcgtagcctgcgcagtgaggatacagcagtctattattgtgcccgttcaccgtcctatatttgctcaggtggcacgtgtgtgtttgatcattggggtcagggtactctggttaccgtttcctctggtggtggtggttcaggtggtggtggtagtggtggagggggtattcaaccggggctgacacaacctcctagcgtgtcgaaaggcctgcgtcagacagcaacgctgacgtgtactggcaatagcaacaatgtggggaatcagggagcagcatggctgcaacagcatcagggtcatccgcctaaactgctgtcttatcgtaacaacgatcgtccgagcgggatttcagaacgttttagtgcctcccgtagcgggaacaccgcttcgctgactatcacggggctgcaaccggaagatgaagccgattattattgctcgacctgggattcttctctgagtgccgtggtctttggtgggggaaccaaactgacagtgctgggacagcctaaagcagcaccttcagcggctgccgaacaaaaactgattagcgaggaggatctgaatggagcggcacatcatcaccatcatcat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z