LOCUS BBa_K195411 2380 bp DNA linear 23-Nov-2024 DEFINITION pTetR+RBS+PelB+F10scFv+C-IgAP+Term ACCESSION BBa_K195411 VERSION BBa_K195411.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..146 /label=PelB misc_feature 81..146 /label=BBa_J32015 CDS 147..986 /label=BBa_K195400 CDS 987..2243 /label=BBa_K195410 misc_feature 81..2243 /label=BBa_K195408 stem_loop 2263..2306 /label=stem_loop misc_feature 2252..2331 /label=BBa_B0010 terminator 2252..2331 /label=BBa_B0010 stem_loop 2347..2366 /label=T7 TE misc_feature 2373..2373 /label=stop polyA_site 2367..2380 /label=polya misc_feature 2340..2380 /label=BBa_B0012 terminator 2340..2380 /label=BBa_B0012 terminator 2252..2380 /label=BBa_B0015 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaatacc tgctgccgac cgctgctgct ggtctgctgc 121 tcctcgctgc ccagccggcg atggcccagg ttcagctggt acaatctggt gccgaagtga 181 aaaaaccggg tagcagtgtg aaagtttcct gtacgagtag cgaggtgacc tttagcagtt 241 tcgccatttc ttgggttcgt caagctcctg gacaaggact ggagtggctg ggtggtatta 301 gcccgatgtt tggaacgcca aattatgccc agaaattcca gggtcgtgtg actattaccg 361 ccgatcagtc aacccgtact gcctatatgg atctgcgtag cctgcgcagt gaggatacag 421 cagtctatta ttgtgcccgt tcaccgtcct atatttgctc aggtggcacg tgtgtgtttg 481 atcattgggg tcagggtact ctggttaccg tttcctctgg tggtggtggt tcaggtggtg 541 gtggtagtgg tggagggggt attcaaccgg ggctgacaca acctcctagc gtgtcgaaag 601 gcctgcgtca gacagcaacg ctgacgtgta ctggcaatag caacaatgtg gggaatcagg 661 gagcagcatg gctgcaacag catcagggtc atccgcctaa actgctgtct tatcgtaaca 721 acgatcgtcc gagcgggatt tcagaacgtt ttagtgcctc ccgtagcggg aacaccgctt 781 cgctgactat cacggggctg caaccggaag atgaagccga ttattattgc tcgacctggg 841 attcttctct gagtgccgtg gtctttggtg ggggaaccaa actgacagtg ctgggacagc 901 ctaaagcagc accttcagcg gctgccgaac aaaaactgat tagcgaggag gatctgaatg 961 gagcggcaca tcatcaccat catcataaag ctgaggaaga agagcaccgt caaacagccc 1021 aatcccagcc gcaacgccgc aaacgccgtg ccgcaccgca ggattatatg gcagtttccc 1081 aagaccgtcc gaaacgtcgc ggacgcagat ctactctgcc ggcaccgccc tcgccatcat 1141 ttgattcatc agcttacgca gcacccaggg ccttgcataa tccggactgg tatgaaaatg 1201 attatgaaga aatccccttg gatgcgctgg aagatgaaga tgtatccgaa tcggttgaca 1261 catcagacaa acagcctcaa gacaatacgg aacttcatga aaaagttgag gcagtgagtt 1321 tgcaaccaag agccgcgcag ccgcgaaccc aagccgccgc gcaagccgat gcagtcagca 1381 ccaatactaa ctcggcttta tctgacgcaa tggcaagcac gcaatctatc ttgttggata 1441 caggtgcttc attaacacgg cacattgcac aaaaatcacg cgctgatgcc gaaaaaaaca 1501 gtgtttggat gtcaaacacc ggttatggcc gtgattatgc ttccgcacaa tatcgccggt 1561 ttagttcgaa acgcacgcaa acacaaatcg gcattgaccg cagcttgtcc gaaaatatgc 1621 agataggcgg agtattgact tactctgaca gtcagcatac ttttgatcag gcgggcggca 1681 aaaatacttt tgtgcaagcc aacctttatg gtaagtatta tttaaatgat gcttggtatg 1741 tggccggcga tattggtgcg ggcagcttga gaagccggtt acaaacgcag caaaaagcaa 1801 actttaaccg aacaagcatc caaaccggcc ttactttggg caatacgctg aaaatcaatc 1861 aattcgagat tgtccctagt gcgggtatcc gttacagccg cctgtcattt gcagattaca 1921 agttgggtga cgacagtgtt aaagtaagtt ctatggcagt gaaaacacta acggccggac 1981 tggattttgc ttatcggttt aaagtcggca accttaccgt aaaacccttg ttatctgccg 2041 cttactttgc caattatggc aaaggcggcg tgaatgtggg cggtaaatcc ttcgcctata 2101 aagcagataa tcaacagcaa tattcagcag gcgccgcgtt actgtaccgt aatgttacat 2161 taaacgtaaa tggcagtatt acaaaaggaa aacaattgga aaaacaaaaa tccggacaaa 2221 ttaaaataca gattcgtttc taatactaga gccaggcatc aaataaaacg aaaggctcag 2281 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 2341 cacactggct caccttcggg tgggcctttc tgcgtttata //