BBa_K195412
type: ComponentDefinition
persistent identity: BBa K195412
display id: BBa_K195412
version: 1
was generated by: iGEM to SBOL conversion
description: This part is composed of RBS,PelB,F10scFv and C-IgAP ,Term
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:16Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: This part is composed of RBS,(PelB+F10scFv+C-IgAP) amd terminator.
discontinued: false
dominant: false
group u list: _292_
m user id: 0
owner id: 4924
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Syue-Liang Lin
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K195409
component: BBa_B0015
sequence annotation: BBa_K195409
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K195412_sequence/1

BBa_K195409
type: Component
persistent identity: component2224244
display id: component2224244
version: 1
top level: BBa_K195412
definition: BBa_K195409
access: public

BBa_B0015
type: Component
persistent identity: component2224251
display id: component2224251
version: 1
top level: BBa_K195412
definition: BBa_B0015
access: public

BBa_K195409
type: SequenceAnnotation
persistent identity: annotation2224244
display id: annotation2224244
version: 1
top level: BBa_K195412
location: https://synbiohub.org/public/igem/BBa_K195412/annotation2224244/range2224244/1
component: BBa_K195409

https://synbiohub.org/public/igem/BBa_K195412/annotation2224244/range2224244/1
type: Range
persistent identity: range2224244
display id: range2224244
version: 1
top level: BBa_K195412
direction: direction/reverse
start: 1
end: 2181
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2224251
display id: annotation2224251
version: 1
top level: BBa_K195412
location: https://synbiohub.org/public/igem/BBa_K195412/annotation2224251/range2224251/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K195412/annotation2224251/range2224251/1
type: Range
persistent identity: range2224251
display id: range2224251
version: 1
top level: BBa_K195412
direction: direction/reverse
start: 2190
end: 2318
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K195409
type: ComponentDefinition
persistent identity: BBa K195409
display id: BBa_K195409
version: 1
was generated by: iGEM to SBOL conversion
description: This part is composed of RBS,PelB,F10scFv and C-IgAP
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:16Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: (RBS)+(PelB+F10scFv+C-IgAP)
discontinued: false
dominant: false
group u list: _292_
m user id: 0
owner id: 4924
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Syue-Liang Lin
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K195408
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K195408
sequence: https://synbiohub.org/public/igem/BBa_K195409_sequence/1

BBa_K195408
type: Component
persistent identity: component2220588
display id: component2220588
version: 1
top level: BBa_K195409
definition: BBa_K195408
access: public

BBa_B0034
type: Component
persistent identity: component2220583
display id: component2220583
version: 1
top level: BBa_K195409
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2220583
display id: annotation2220583
version: 1
top level: BBa_K195409
location: https://synbiohub.org/public/igem/BBa_K195409/annotation2220583/range2220583/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K195409/annotation2220583/range2220583/1
type: Range
persistent identity: range2220583
display id: range2220583
version: 1
top level: BBa_K195409
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K195408
type: SequenceAnnotation
persistent identity: annotation2220588
display id: annotation2220588
version: 1
top level: BBa_K195409
location: https://synbiohub.org/public/igem/BBa_K195409/annotation2220588/range2220588/1
component: BBa_K195408

https://synbiohub.org/public/igem/BBa_K195409/annotation2220588/range2220588/1
type: Range
persistent identity: range2220588
display id: range2220588
version: 1
top level: BBa_K195409
direction: direction/reverse
start: 19
end: 2181
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K195408
type: ComponentDefinition
persistent identity: BBa K195408
display id: BBa_K195408
version: 1
was generated by: iGEM to SBOL conversion
description: This part is composed of PelB,F10scFv and C-IgAP
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:16Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: scFv fusion with C-IgAP and PelB|
discontinued: false
dominant: false
group u list: _292_
m user id: 0
owner id: 4924
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Syue-Liang Lin
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J32015
component: BBa_K195410
component: BBa_K195400
sequence annotation: BBa_J32015
sequence annotation: BBa_K195400
sequence annotation: BBa_K195410
sequence: https://synbiohub.org/public/igem/BBa_K195408_sequence/1

BBa_J32015
type: Component
persistent identity: component2059139
display id: component2059139
version: 1
top level: BBa_K195408
definition: PelB
access: public

BBa_K195410
type: Component
persistent identity: component2059141
display id: component2059141
version: 1
top level: BBa_K195408
definition: C-IgAP
access: public

BBa_K195400
type: Component
persistent identity: component2059140
display id: component2059140
version: 1
top level: BBa_K195408
definition: F10scFv
access: public

BBa_J32015
type: SequenceAnnotation
persistent identity: annotation2059139
display id: annotation2059139
version: 1
top level: BBa_K195408
location: https://synbiohub.org/public/igem/BBa_K195408/annotation2059139/range2059139/1
component: BBa_J32015

https://synbiohub.org/public/igem/BBa_K195408/annotation2059139/range2059139/1
type: Range
persistent identity: range2059139
display id: range2059139
version: 1
top level: BBa_K195408
direction: direction/reverse
start: 1
end: 66
orientation: inline

BBa_K195400
type: SequenceAnnotation
persistent identity: annotation2059140
display id: annotation2059140
version: 1
top level: BBa_K195408
location: https://synbiohub.org/public/igem/BBa_K195408/annotation2059140/range2059140/1
component: BBa_K195400

https://synbiohub.org/public/igem/BBa_K195408/annotation2059140/range2059140/1
type: Range
persistent identity: range2059140
display id: range2059140
version: 1
top level: BBa_K195408
direction: direction/reverse
start: 67
end: 906
orientation: inline

BBa_K195410
type: SequenceAnnotation
persistent identity: annotation2059141
display id: annotation2059141
version: 1
top level: BBa_K195408
location: https://synbiohub.org/public/igem/BBa_K195408/annotation2059141/range2059141/1
component: BBa_K195410

https://synbiohub.org/public/igem/BBa_K195408/annotation2059141/range2059141/1
type: Range
persistent identity: range2059141
display id: range2059141
version: 1
top level: BBa_K195408
direction: direction/reverse
start: 907
end: 2163
orientation: inline

F10scFv
type: ComponentDefinition
persistent identity: BBa K195400
display id: BBa_K195400
version: 1
was generated by: iGEM to SBOL conversion
description: F10scFv
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:16Z
mutable provenance: synthesized by Mr.gene
owned by: james
owned by: myers
mutable description: Our antibody (F10scFv) can bind to the highly conserved region in HA on many subtypes of the Influenza A virus. F10scFv binds to a common epitope that is a highly conserved pocket in the stem region of Hemagglutinin containing the fusion peptide.
discontinued: false
dominant: false
experience: experience/None
group u list: _292_
m user id: 0
owner id: 4924
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Syue-Liang Lin
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K195400_sequence/1

C-IgAP
type: ComponentDefinition
persistent identity: BBa K195410
display id: BBa_K195410
version: 1
was generated by: iGEM to SBOL conversion
description: C-terminal of IgA protease
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:16Z
mutable provenance: Neisseria gonorrhoeae FA1090
owned by: james
owned by: myers
mutable description: When in the periplasm, the beta-domain(C-terminal) of the protein inserts into the outer membrane to form a beta-barrel structure. After formation of the beta-barrel pore, the passenger domain is translocated to the cell surface through the pore. Once at the cell surface, several possibilities may occur.
discontinued: false
dominant: false
experience: experience/None
group u list: _292_
m user id: 0
owner id: 4924
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: C-IgAP(BBa_J32018) is exist part,but this part is not exist in plate. We need to PCR this sequence from Neisseria gonorrhoeae by ourself. There have some problem:Pst1 cutting site...So we have to mutate these site dierectly.
star: false
creator: Syue-Liang Lin
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K195410_sequence/1

PelB
type: ComponentDefinition
persistent identity: BBa J32015
display id: BBa_J32015
version: 1
was generated by: iGEM to SBOL conversion
description: PelB leader sequence; directs protein to E. coli periplasmic membrane
created: 2006-08-24T11:00:00Z
modified: 2015-08-31T04:08:46Z
mutable provenance: Novagen
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The pelB leader sequence is a sequence of amino acids which when attached to a protein, directs the protein to the periplasmic membrane of E. coli, where the sequence is removed by pelB peptidase. It is used to direct coat protein-antigen fusions to the cell surface.
discontinued: false
dominant: true
experience: experience/None
group u list: _50_
m user id: 0
owner id: 495
owning group id: 50
sample status: In stock
status: status/Available
bookmark: true
mutable notes: .
star: true
creator: Austen Heinz
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence annotation: PelB
sequence: https://synbiohub.org/public/igem/BBa_J32015_sequence/1

PelB
type: SequenceAnnotation
persistent identity: annotation1898182
display id: annotation1898182
version: 1
top level: PelB
location: https://synbiohub.org/public/igem/BBa_J32015/annotation1898182/range1898182/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_J32015/annotation1898182/range1898182/1
type: Range
persistent identity: range1898182
display id: range1898182
version: 1
top level: PelB
direction: direction/forward
start: 1
end: 66
orientation: inline

https://synbiohub.org/public/igem/BBa_K195412_sequence/1
type: Sequence
persistent identity: BBa K195412 sequence
display id: BBa_K195412_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcccaggttcagctggtacaatctggtgccgaagtgaaaaaaccgggtagcagtgtgaaagtttcctgtacgagtagcgaggtgacctttagcagtttcgccatttcttgggttcgtcaagctcctggacaaggactggagtggctgggtggtattagcccgatgtttggaacgccaaattatgcccagaaattccagggtcgtgtgactattaccgccgatcagtcaacccgtactgcctatatggatctgcgtagcctgcgcagtgaggatacagcagtctattattgtgcccgttcaccgtcctatatttgctcaggtggcacgtgtgtgtttgatcattggggtcagggtactctggttaccgtttcctctggtggtggtggttcaggtggtggtggtagtggtggagggggtattcaaccggggctgacacaacctcctagcgtgtcgaaaggcctgcgtcagacagcaacgctgacgtgtactggcaatagcaacaatgtggggaatcagggagcagcatggctgcaacagcatcagggtcatccgcctaaactgctgtcttatcgtaacaacgatcgtccgagcgggatttcagaacgttttagtgcctcccgtagcgggaacaccgcttcgctgactatcacggggctgcaaccggaagatgaagccgattattattgctcgacctgggattcttctctgagtgccgtggtctttggtgggggaaccaaactgacagtgctgggacagcctaaagcagcaccttcagcggctgccgaacaaaaactgattagcgaggaggatctgaatggagcggcacatcatcaccatcatcataaagctgaggaagaagagcaccgtcaaacagcccaatcccagccgcaacgccgcaaacgccgtgccgcaccgcaggattatatggcagtttcccaagaccgtccgaaacgtcgcggacgcagatctactctgccggcaccgccctcgccatcatttgattcatcagcttacgcagcacccagggccttgcataatccggactggtatgaaaatgattatgaagaaatccccttggatgcgctggaagatgaagatgtatccgaatcggttgacacatcagacaaacagcctcaagacaatacggaacttcatgaaaaagttgaggcagtgagtttgcaaccaagagccgcgcagccgcgaacccaagccgccgcgcaagccgatgcagtcagcaccaatactaactcggctttatctgacgcaatggcaagcacgcaatctatcttgttggatacaggtgcttcattaacacggcacattgcacaaaaatcacgcgctgatgccgaaaaaaacagtgtttggatgtcaaacaccggttatggccgtgattatgcttccgcacaatatcgccggtttagttcgaaacgcacgcaaacacaaatcggcattgaccgcagcttgtccgaaaatatgcagataggcggagtattgacttactctgacagtcagcatacttttgatcaggcgggcggcaaaaatacttttgtgcaagccaacctttatggtaagtattatttaaatgatgcttggtatgtggccggcgatattggtgcgggcagcttgagaagccggttacaaacgcagcaaaaagcaaactttaaccgaacaagcatccaaaccggccttactttgggcaatacgctgaaaatcaatcaattcgagattgtccctagtgcgggtatccgttacagccgcctgtcatttgcagattacaagttgggtgacgacagtgttaaagtaagttctatggcagtgaaaacactaacggccggactggattttgcttatcggtttaaagtcggcaaccttaccgtaaaacccttgttatctgccgcttactttgccaattatggcaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K195409_sequence/1
type: Sequence
persistent identity: BBa K195409 sequence
display id: BBa_K195409_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcccaggttcagctggtacaatctggtgccgaagtgaaaaaaccgggtagcagtgtgaaagtttcctgtacgagtagcgaggtgacctttagcagtttcgccatttcttgggttcgtcaagctcctggacaaggactggagtggctgggtggtattagcccgatgtttggaacgccaaattatgcccagaaattccagggtcgtgtgactattaccgccgatcagtcaacccgtactgcctatatggatctgcgtagcctgcgcagtgaggatacagcagtctattattgtgcccgttcaccgtcctatatttgctcaggtggcacgtgtgtgtttgatcattggggtcagggtactctggttaccgtttcctctggtggtggtggttcaggtggtggtggtagtggtggagggggtattcaaccggggctgacacaacctcctagcgtgtcgaaaggcctgcgtcagacagcaacgctgacgtgtactggcaatagcaacaatgtggggaatcagggagcagcatggctgcaacagcatcagggtcatccgcctaaactgctgtcttatcgtaacaacgatcgtccgagcgggatttcagaacgttttagtgcctcccgtagcgggaacaccgcttcgctgactatcacggggctgcaaccggaagatgaagccgattattattgctcgacctgggattcttctctgagtgccgtggtctttggtgggggaaccaaactgacagtgctgggacagcctaaagcagcaccttcagcggctgccgaacaaaaactgattagcgaggaggatctgaatggagcggcacatcatcaccatcatcataaagctgaggaagaagagcaccgtcaaacagcccaatcccagccgcaacgccgcaaacgccgtgccgcaccgcaggattatatggcagtttcccaagaccgtccgaaacgtcgcggacgcagatctactctgccggcaccgccctcgccatcatttgattcatcagcttacgcagcacccagggccttgcataatccggactggtatgaaaatgattatgaagaaatccccttggatgcgctggaagatgaagatgtatccgaatcggttgacacatcagacaaacagcctcaagacaatacggaacttcatgaaaaagttgaggcagtgagtttgcaaccaagagccgcgcagccgcgaacccaagccgccgcgcaagccgatgcagtcagcaccaatactaactcggctttatctgacgcaatggcaagcacgcaatctatcttgttggatacaggtgcttcattaacacggcacattgcacaaaaatcacgcgctgatgccgaaaaaaacagtgtttggatgtcaaacaccggttatggccgtgattatgcttccgcacaatatcgccggtttagttcgaaacgcacgcaaacacaaatcggcattgaccgcagcttgtccgaaaatatgcagataggcggagtattgacttactctgacagtcagcatacttttgatcaggcgggcggcaaaaatacttttgtgcaagccaacctttatggtaagtattatttaaatgatgcttggtatgtggccggcgatattggtgcgggcagcttgagaagccggttacaaacgcagcaaaaagcaaactttaaccgaacaagcatccaaaccggccttactttgggcaatacgctgaaaatcaatcaattcgagattgtccctagtgcgggtatccgttacagccgcctgtcatttgcagattacaagttgggtgacgacagtgttaaagtaagttctatggcagtgaaaacactaacggccggactggattttgcttatcggtttaaagtcggcaaccttaccgtaaaacccttgttatctgccgcttactttgccaattatggcaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K195408_sequence/1
type: Sequence
persistent identity: BBa K195408 sequence
display id: BBa_K195408_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcccaggttcagctggtacaatctggtgccgaagtgaaaaaaccgggtagcagtgtgaaagtttcctgtacgagtagcgaggtgacctttagcagtttcgccatttcttgggttcgtcaagctcctggacaaggactggagtggctgggtggtattagcccgatgtttggaacgccaaattatgcccagaaattccagggtcgtgtgactattaccgccgatcagtcaacccgtactgcctatatggatctgcgtagcctgcgcagtgaggatacagcagtctattattgtgcccgttcaccgtcctatatttgctcaggtggcacgtgtgtgtttgatcattggggtcagggtactctggttaccgtttcctctggtggtggtggttcaggtggtggtggtagtggtggagggggtattcaaccggggctgacacaacctcctagcgtgtcgaaaggcctgcgtcagacagcaacgctgacgtgtactggcaatagcaacaatgtggggaatcagggagcagcatggctgcaacagcatcagggtcatccgcctaaactgctgtcttatcgtaacaacgatcgtccgagcgggatttcagaacgttttagtgcctcccgtagcgggaacaccgcttcgctgactatcacggggctgcaaccggaagatgaagccgattattattgctcgacctgggattcttctctgagtgccgtggtctttggtgggggaaccaaactgacagtgctgggacagcctaaagcagcaccttcagcggctgccgaacaaaaactgattagcgaggaggatctgaatggagcggcacatcatcaccatcatcataaagctgaggaagaagagcaccgtcaaacagcccaatcccagccgcaacgccgcaaacgccgtgccgcaccgcaggattatatggcagtttcccaagaccgtccgaaacgtcgcggacgcagatctactctgccggcaccgccctcgccatcatttgattcatcagcttacgcagcacccagggccttgcataatccggactggtatgaaaatgattatgaagaaatccccttggatgcgctggaagatgaagatgtatccgaatcggttgacacatcagacaaacagcctcaagacaatacggaacttcatgaaaaagttgaggcagtgagtttgcaaccaagagccgcgcagccgcgaacccaagccgccgcgcaagccgatgcagtcagcaccaatactaactcggctttatctgacgcaatggcaagcacgcaatctatcttgttggatacaggtgcttcattaacacggcacattgcacaaaaatcacgcgctgatgccgaaaaaaacagtgtttggatgtcaaacaccggttatggccgtgattatgcttccgcacaatatcgccggtttagttcgaaacgcacgcaaacacaaatcggcattgaccgcagcttgtccgaaaatatgcagataggcggagtattgacttactctgacagtcagcatacttttgatcaggcgggcggcaaaaatacttttgtgcaagccaacctttatggtaagtattatttaaatgatgcttggtatgtggccggcgatattggtgcgggcagcttgagaagccggttacaaacgcagcaaaaagcaaactttaaccgaacaagcatccaaaccggccttactttgggcaatacgctgaaaatcaatcaattcgagattgtccctagtgcgggtatccgttacagccgcctgtcatttgcagattacaagttgggtgacgacagtgttaaagtaagttctatggcagtgaaaacactaacggccggactggattttgcttatcggtttaaagtcggcaaccttaccgtaaaacccttgttatctgccgcttactttgccaattatggcaaaggcggcgtgaatgtggg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K195400_sequence/1
type: Sequence
persistent identity: BBa K195400 sequence
display id: BBa_K195400_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caggttcagctggtacaatctggtgccgaagtgaaaaaaccgggtagcagtgtgaaagtttcctgtacgagtagcgaggtgacctttagcagtttcgccatttcttgggttcgtcaagctcctggacaaggactggagtggctgggtggtattagcccgatgtttggaacgccaaattatgcccagaaattccagggtcgtgtgactattaccgccgatcagtcaacccgtactgcctatatggatctgcgtagcctgcgcagtgaggatacagcagtctattattgtgcccgttcaccgtcctatatttgctcaggtggcacgtgtgtgtttgatcattggggtcagggtactctggttaccgtttcctctggtggtggtggttcaggtggtggtggtagtggtggagggggtattcaaccggggctgacacaacctcctagcgtgtcgaaaggcctgcgtcagacagcaacgctgacgtgtactggcaatagcaacaatgtggggaatcagggagcagcatggctgcaacagcatcagggtcatccgcctaaactgctgtcttatcgtaacaacgatcgtccgagcgggatttcagaacgttttagtgcctcccgtagcgggaacaccgcttcgctgactatcacggggctgcaaccggaagatgaagccgattattattgctcgacctgggattcttctctgagtgccgtggtctttggtgggggaaccaaactgacagtgctgggacagcctaaagcagcaccttcagcggctgccgaacaaaaactgattagcgaggaggatctgaatggagcggcacatcatcaccatcatcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K195410_sequence/1
type: Sequence
persistent identity: BBa K195410 sequence
display id: BBa_K195410_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagctgaggaagaagagcaccgtcaaacagcccaatcccagccgcaacgccgcaaacgccgtgccgcaccgcaggattatatggcagtttcccaagaccgtccgaaacgtcgcggacgcagatctactctgccggcaccgccctcgccatcatttgattcatcagcttacgcagcacccagggccttgcataatccggactggtatgaaaatgattatgaagaaatccccttggatgcgctggaagatgaagatgtatccgaatcggttgacacatcagacaaacagcctcaagacaatacggaacttcatgaaaaagttgaggcagtgagtttgcaaccaagagccgcgcagccgcgaacccaagccgccgcgcaagccgatgcagtcagcaccaatactaactcggctttatctgacgcaatggcaagcacgcaatctatcttgttggatacaggtgcttcattaacacggcacattgcacaaaaatcacgcgctgatgccgaaaaaaacagtgtttggatgtcaaacaccggttatggccgtgattatgcttccgcacaatatcgccggtttagttcgaaacgcacgcaaacacaaatcggcattgaccgcagcttgtccgaaaatatgcagataggcggagtattgacttactctgacagtcagcatacttttgatcaggcgggcggcaaaaatacttttgtgcaagccaacctttatggtaagtattatttaaatgatgcttggtatgtggccggcgatattggtgcgggcagcttgagaagccggttacaaacgcagcaaaaagcaaactttaaccgaacaagcatccaaaccggccttactttgggcaatacgctgaaaatcaatcaattcgagattgtccctagtgcgggtatccgttacagccgcctgtcatttgcagattacaagttgggtgacgacagtgttaaagtaagttctatggcagtgaaaacactaacggccggactggattttgcttatcggtttaaagtcggcaaccttaccgtaaaacccttgttatctgccgcttactttgccaattatggcaaaggcggcgtgaatgtgggcggtaaatccttcgcctataaagcagataatcaacagcaatattcagcaggcgccgcgttactgtaccgtaatgttacattaaacgtaaatggcagtattacaaaaggaaaacaattggaaaaacaaaaatccggacaaattaaaatacagattcgtttctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J32015_sequence/1
type: Sequence
persistent identity: BBa J32015 sequence
display id: BBa_J32015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z