LOCUS       BBa_K195413             2380 bp    DNA     linear       23-Dec-2024
DEFINITION  All in one
ACCESSION   BBa_K195413
VERSION     BBa_K195413.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..146
                     /label=PelB
     misc_feature    81..146
                     /label=BBa_J32015
     CDS             147..986
                     /label=BBa_K195400
     CDS             987..2243
                     /label=BBa_K195410
     misc_feature    81..2243
                     /label=BBa_K195408
     stem_loop       2263..2306
                     /label=stem_loop
     misc_feature    2252..2331
                     /label=BBa_B0010
     terminator      2252..2331
                     /label=BBa_B0010
     stem_loop       2347..2366
                     /label=T7 TE
     misc_feature    2373..2373
                     /label=stop
     polyA_site      2367..2380
                     /label=polya
     misc_feature    2340..2380
                     /label=BBa_B0012
     terminator      2340..2380
                     /label=BBa_B0012
     terminator      2252..2380
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaatacc tgctgccgac cgctgctgct ggtctgctgc
      121 tcctcgctgc ccagccggcg atggcccagg ttcagctggt acaatctggt gccgaagtga
      181 aaaaaccggg tagcagtgtg aaagtttcct gtacgagtag cgaggtgacc tttagcagtt
      241 tcgccatttc ttgggttcgt caagctcctg gacaaggact ggagtggctg ggtggtatta
      301 gcccgatgtt tggaacgcca aattatgccc agaaattcca gggtcgtgtg actattaccg
      361 ccgatcagtc aacccgtact gcctatatgg atctgcgtag cctgcgcagt gaggatacag
      421 cagtctatta ttgtgcccgt tcaccgtcct atatttgctc aggtggcacg tgtgtgtttg
      481 atcattgggg tcagggtact ctggttaccg tttcctctgg tggtggtggt tcaggtggtg
      541 gtggtagtgg tggagggggt attcaaccgg ggctgacaca acctcctagc gtgtcgaaag
      601 gcctgcgtca gacagcaacg ctgacgtgta ctggcaatag caacaatgtg gggaatcagg
      661 gagcagcatg gctgcaacag catcagggtc atccgcctaa actgctgtct tatcgtaaca
      721 acgatcgtcc gagcgggatt tcagaacgtt ttagtgcctc ccgtagcggg aacaccgctt
      781 cgctgactat cacggggctg caaccggaag atgaagccga ttattattgc tcgacctggg
      841 attcttctct gagtgccgtg gtctttggtg ggggaaccaa actgacagtg ctgggacagc
      901 ctaaagcagc accttcagcg gctgccgaac aaaaactgat tagcgaggag gatctgaatg
      961 gagcggcaca tcatcaccat catcataaag ctgaggaaga agagcaccgt caaacagccc
     1021 aatcccagcc gcaacgccgc aaacgccgtg ccgcaccgca ggattatatg gcagtttccc
     1081 aagaccgtcc gaaacgtcgc ggacgcagat ctactctgcc ggcaccgccc tcgccatcat
     1141 ttgattcatc agcttacgca gcacccaggg ccttgcataa tccggactgg tatgaaaatg
     1201 attatgaaga aatccccttg gatgcgctgg aagatgaaga tgtatccgaa tcggttgaca
     1261 catcagacaa acagcctcaa gacaatacgg aacttcatga aaaagttgag gcagtgagtt
     1321 tgcaaccaag agccgcgcag ccgcgaaccc aagccgccgc gcaagccgat gcagtcagca
     1381 ccaatactaa ctcggcttta tctgacgcaa tggcaagcac gcaatctatc ttgttggata
     1441 caggtgcttc attaacacgg cacattgcac aaaaatcacg cgctgatgcc gaaaaaaaca
     1501 gtgtttggat gtcaaacacc ggttatggcc gtgattatgc ttccgcacaa tatcgccggt
     1561 ttagttcgaa acgcacgcaa acacaaatcg gcattgaccg cagcttgtcc gaaaatatgc
     1621 agataggcgg agtattgact tactctgaca gtcagcatac ttttgatcag gcgggcggca
     1681 aaaatacttt tgtgcaagcc aacctttatg gtaagtatta tttaaatgat gcttggtatg
     1741 tggccggcga tattggtgcg ggcagcttga gaagccggtt acaaacgcag caaaaagcaa
     1801 actttaaccg aacaagcatc caaaccggcc ttactttggg caatacgctg aaaatcaatc
     1861 aattcgagat tgtccctagt gcgggtatcc gttacagccg cctgtcattt gcagattaca
     1921 agttgggtga cgacagtgtt aaagtaagtt ctatggcagt gaaaacacta acggccggac
     1981 tggattttgc ttatcggttt aaagtcggca accttaccgt aaaacccttg ttatctgccg
     2041 cttactttgc caattatggc aaaggcggcg tgaatgtggg cggtaaatcc ttcgcctata
     2101 aagcagataa tcaacagcaa tattcagcag gcgccgcgtt actgtaccgt aatgttacat
     2161 taaacgtaaa tggcagtatt acaaaaggaa aacaattgga aaaacaaaaa tccggacaaa
     2221 ttaaaataca gattcgtttc taatactaga gccaggcatc aaataaaacg aaaggctcag
     2281 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     2341 cacactggct caccttcggg tgggcctttc tgcgtttata
//