BBa_K1955002 1 BBa_K1955002 Leishmania- 3 prime UTR 2016-10-13T11:00:00Z 2016-10-17T07:14:28Z The Leish-3'UTR sequence originally came from Leishmania genome and is acquired from GenBank. The specific terminator function in Leishmania genome has not been elucidated yet. We selected the 3??? untranslated region of a highly expressed gene, NAGT, to be the transcription terminator and other extra function modifications needed for Leishmania protein expression termination. This biobrick needs to use with the Leish-5'UTR-HYG that we provide as the regulation of protein expression and also the drug selection system for the Leishmania to perform a complete protein expression system in Leishamnia. Add the protein sequence in between the Leish-5'UTR-HYG and Leish-3'UTR for Leishmania to express your target protein. false false _2422_ 30462 30553 9 false The Leish-3???UTR is a 774 bp DNA sequence, which is directly synthesized by IDT. We acquired the original leishmania genome sequence of NAGT coding region and intergenic region from NCBI (GenBank accession# L11705), and choose the 3???UTR region of the sequence as the transcription terminator and protein expression regulator in our expression vector. Some nucleotides had been changed to avoid the restriction site. The function of the 3???UTR intergenic region was studied and published by Dr. Kwang-Poo Chang (Gene 196 (1997) 49???59). false Justine Hsu BBa_K1955000 1 BBa_K1955000 pSB1C3-Hemagglutinin 2016-10-13T11:00:00Z 2016-10-20T01:16:22Z The Leish-5'UTR sequence originally came from Leishmania genome and is acquired from GenBank. The Hygromycin resistant gene sequence is also acquired from GenBank. The promoter and the ribosomal binding site of Leishmania genome has not been elucidated yet. We selected the 5'- untranslated region of a highly expressed gene, P36, to be the promoter, RBS binding site and other extra function needed for Leishmania protein expression. We also added a Hygromycin resistant gene as drug selection marker. AS a dual functional biobrick of regulatory and selection marker, we provide the user the regulation of protein expression and also the drug selection system that is most commonly and effectively used in Leishmania experiments. This biobrick needs to use with the 3'UTR that we provide as the terminator of the Leishmania to perform a complete protein expression system for Leishamnia. Add the protein sequence in between the Leish-5'UTR-HYG and Leish-3'UTR for leishmania to expression your target protein. false false _2422_ 30427 30553 9 true The Leish-5???UTR-HYG is a 1446 bp DNA sequence, which is directly synthesized by IDT. We acquired the original leishmania genome sequence of p36 coding region and intergenic region from NCBI (GenBank accession# M96635), replacing the p36 coding sequence into hygromycin resistant gene, and keep the flanks and intergenic sequence in order to provide a selection marker for our leishmania expression vector. Some nucleotides of Leish-5???UTR-HYG sequence had been changed to avoid the restriction sites. The function of the 5???UTR intergenic region was studied and published by Dr. Kwang-Poo Chang (Gene 196 (1997) 49???59). false Justine Hsu BBa_K1955003 1 BBa_K1955003 Leishmania-5 prime UTR-Hygromycin 2016-10-13T11:00:00Z 2016-10-19T03:32:18Z The whole sequence of Hemagglutinin (HA) from H1N1 is acquired from GenBank. The coding sequence of Hemagglutinin (HA) from H1N1 acquired from NCBI. HA is a glycoprotein found on the surface of influenza viruses and can be used in immunological experiments as the target antigen. false false _2422_ 30427 30553 9 false The hemagglutinin (HA) part is a 1700 bp DNA sequence acquired from GenBank (Accession# J02176.1). Some nucleotides of HA sequence had been changed to avoid the restriction sites. In order to compare the protein expression efficiency of our expression vector and the original pX63HYG shuttle vector, we add BamHI restriction site at the head and tail of HA coding sequence for the ligation of pX63HYG shuttle vector and HA sequence. false Justine Hsu BBa_K1955005 1 BBa_K1955005 pSB1C3-5 2016-10-16T11:00:00Z 2016-10-19T06:27:04Z The whole sequence of Ovalbumin (OVA) is acquired from GenBank (Accession# AH002466.2). The complete coding sequence of Ovalbumin (OVA) acquired from GenBank (Accession# AH002466.2) . OVA has been commonly used as the antigen for testing the efficiency of antibody response and T cell activation in previous immunology experiments, thus is a easy to use antigen. false false _2422_ 30427 30462 9 false The Ovalbumin (OVA) sequence is a 2098 bp DNA sequence acquired from GenBank (Accession# AH002466.2). We changed several nucleotides of the sequence to avoid the restriction sites and separate the sequence into two parts since it???s too long to synthesis. The restriction site between these two parts is SapI. After cutting the sequence with EcoRI, SapI and PstI, we ligated the two parts with pSB1C3 and confirmed the OVA sequence is correct by DNA sequencing. In order to compare the protein expression efficiency of our expression vector and the original pX63HYG shuttle vector, we added BamHI restriction site at the head and tail of OVA coding sequence for the ligation of pX63HYG shuttle vector and OVA sequence. false HSU, CHIEN-YU component2523356 1 BBa_K1955000 component2523357 1 BBa_K1955002 component2523355 1 BBa_K1955003 annotation2523357 1 BBa_K1955002 range2523357 1 3178 3951 annotation2523355 1 BBa_K1955003 range2523355 1 1 1446 annotation2523356 1 BBa_K1955000 range2523356 1 1455 3169 BBa_K1955003_sequence 1 aaggcgcacgttgcttctcctccctccctcccctctcttgtgtcggcttgccaaaccccattcatggctgctccgttgccggtttctttttctttctcgcccgtccttcaacccccctgtccttctcattttattgttggcaccacccatcggtcacttcctccctcaccctcccaaccactcacacccacccacacacacacgcacttgcatagaacgtcagcgcttcctatcgagacacacactctcaaacacgagtggtccagtcatcacccgcacactccactctcatttttctttcgcagtatcatctctctaatctctcacggaggtatttctcactcgatatacacacgcgtgcataccgcttccctaacccactccacacacacacacacactggcgcacgtcctctgaaaaatgaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggagttcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttcttcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatag BBa_K1955005_sequence 1 aaggcgcacgttgcttctcctccctccctcccctctcttgtgtcggcttgccaaaccccattcatggctgctccgttgccggtttctttttctttctcgcccgtccttcaacccccctgtccttctcattttattgttggcaccacccatcggtcacttcctccctcaccctcccaaccactcacacccacccacacacacacgcacttgcatagaacgtcagcgcttcctatcgagacacacactctcaaacacgagtggtccagtcatcacccgcacactccactctcatttttctttcgcagtatcatctctctaatctctcacggaggtatttctcactcgatatacacacgcgtgcataccgcttccctaacccactccacacacacacacacactggcgcacgtcctctgaaaaatgaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggagttcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttcttcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagtactagagcaaggatcccgatgaaggcttttgtactggtcctgttatatgcatttgtagctacagatgcagacacaatatgtataggctaccatgcgaacaactcaaccgacactgttgacacaatattcgagaagaatgtggcagtgacacattctgttaacctgctcgaagacagacacaacgggaaactatgtaaattaaaaggaatagccccactacaattggggaaatgtaacatcaccggatggctcttgggaaatccagaatgcgactcactgcttccagcgagatcatggtcctacattgtagaaacaccaaactctgagaatggagcatgttatccaggagatttcatcgactatgaggaactgagggagcaattgagctcagtatcatcattagaaagattcgaaatatttcccaaggaaagttcatggcccaaccacacattcaacggagtaacagtatcatgctcccataggggaaaaagcagtttttacagaaatttgctatggctgacgaagaagggggattcatacccaaagctgaccaattcctatgtgaacaataaagggaaagaagtccttgtactatggggtgttcatcacccgtctagcagtgatgagcaacagagtctctatagtaatggaaatgcttatgtctctgtagcgtcttcaaattataacaggagattcaccccggaaatagctgcaaggcccaaagtaaaagatcaacatgggaggatgaactattactggaccttgctagaacccggagacacaataatatttgaggcaactggtaatctaatagcaccatggtatgctttcgcactgagtagagggtttgagtccggcatcatcacctcaaacgcgtcaatgcatgagtgtaacacgaagtgtcaaacaccccagggatctataaacagcaatctccctttccagaatatacacccagtcacaataggagagtgcccaaaatatgtcaggagtaccaaattgaggatggttacaggactaagaaacatcccatccattcaatacagaggtctatttggagccattgctggttttattgaggggggatggactggaatgatagatggatggtatggttatcatcatcagaatgaacagggatcaggctatgcagcggatcaaaaaagcacacagaatgccattaacaggattacaaacaaggtgaactctgttatcgagaaaatgaacactcaattcaccgctgtgggtaaagagttcaacaacttagaaaaaaggatggaaaatttaaataaaaaagttgatgatgggtttctggacatttggacatataatgcagaattgttagttctactggaaaatgaaagaactttggatttccatgacttaaatgtgaagaatctgtacgagaaagtaaaaagccaattaaagaataatgccaaagaaatcggaaatgggtgttttgagttctaccacaagtgtgacaatgaatgcatggaaagtgtaagaaatgggacttatgattatccaaaatattcagaagaatcaaagttgaacagggaaaagatagatggagtgaaattggaatcaatgggggtgtatcagattctggcgatctactcaactgtcgccagttcactggtgcttttggtctccctgggggcaatcagtttctggatgtgttctaatgggtctttgcagtgcagaatatgcatctgaggatcctactagagagccaggtttgaaggacgcgcaagagaggccatgcactgtgctgttgagtttttgttgctgttttcttgttttcacccctgtgtgtgcgggtgtcgattgcccttgaatgcgcacattgcgctccgagaaggctcgccgatggggtgaaacgaggactgctgacgctgacattgtgaacatgcgtgcagccatcccgtccgacgtttctccacctcgatggcacggccacgcaccaaaagcgagatcctgctgtaaggaagcagacgtattccttggctgcacagtgagcactgtcccccatgcactgctggcgtgcaccctatgtttaaaggagaacgaacacacaaacaggcatcttccgagcactccgctgttctgcactggagggggcggggcgcgaaaaaaaaagagaaaagcgtctcagtgactgacgtatgtcttcgcttttcctttctggatggtgccagcgttggctgccacctatctgccccttgtttcgcaaatggggcattgctttcctgaacctcctcgtccctccgtgtgtatgcatcggcgtgtttgtgtatgggcatgtatgcatatctgggctcctgagtcacggttcgcttctcttcgtgctctctctctactcgtatccattggtgtgtgcgtctccgccacgtagaggtgctgtgtggcgttattcggctcgtgtgtttgtgtgtggtgtgctaggttttgcttccgcggtgcgtccgtcacaacttcatccctcatcctcccttctaccactt BBa_K1955000_sequence 1 caaggatcccgatgaaggcttttgtactggtcctgttatatgcatttgtagctacagatgcagacacaatatgtataggctaccatgcgaacaactcaaccgacactgttgacacaatattcgagaagaatgtggcagtgacacattctgttaacctgctcgaagacagacacaacgggaaactatgtaaattaaaaggaatagccccactacaattggggaaatgtaacatcaccggatggctcttgggaaatccagaatgcgactcactgcttccagcgagatcatggtcctacattgtagaaacaccaaactctgagaatggagcatgttatccaggagatttcatcgactatgaggaactgagggagcaattgagctcagtatcatcattagaaagattcgaaatatttcccaaggaaagttcatggcccaaccacacattcaacggagtaacagtatcatgctcccataggggaaaaagcagtttttacagaaatttgctatggctgacgaagaagggggattcatacccaaagctgaccaattcctatgtgaacaataaagggaaagaagtccttgtactatggggtgttcatcacccgtctagcagtgatgagcaacagagtctctatagtaatggaaatgcttatgtctctgtagcgtcttcaaattataacaggagattcaccccggaaatagctgcaaggcccaaagtaaaagatcaacatgggaggatgaactattactggaccttgctagaacccggagacacaataatatttgaggcaactggtaatctaatagcaccatggtatgctttcgcactgagtagagggtttgagtccggcatcatcacctcaaacgcgtcaatgcatgagtgtaacacgaagtgtcaaacaccccagggatctataaacagcaatctccctttccagaatatacacccagtcacaataggagagtgcccaaaatatgtcaggagtaccaaattgaggatggttacaggactaagaaacatcccatccattcaatacagaggtctatttggagccattgctggttttattgaggggggatggactggaatgatagatggatggtatggttatcatcatcagaatgaacagggatcaggctatgcagcggatcaaaaaagcacacagaatgccattaacaggattacaaacaaggtgaactctgttatcgagaaaatgaacactcaattcaccgctgtgggtaaagagttcaacaacttagaaaaaaggatggaaaatttaaataaaaaagttgatgatgggtttctggacatttggacatataatgcagaattgttagttctactggaaaatgaaagaactttggatttccatgacttaaatgtgaagaatctgtacgagaaagtaaaaagccaattaaagaataatgccaaagaaatcggaaatgggtgttttgagttctaccacaagtgtgacaatgaatgcatggaaagtgtaagaaatgggacttatgattatccaaaatattcagaagaatcaaagttgaacagggaaaagatagatggagtgaaattggaatcaatgggggtgtatcagattctggcgatctactcaactgtcgccagttcactggtgcttttggtctccctgggggcaatcagtttctggatgtgttctaatgggtctttgcagtgcagaatatgcatctgaggatcc BBa_K1955002_sequence 1 agccaggtttgaaggacgcgcaagagaggccatgcactgtgctgttgagtttttgttgctgttttcttgttttcacccctgtgtgtgcgggtgtcgattgcccttgaatgcgcacattgcgctccgagaaggctcgccgatggggtgaaacgaggactgctgacgctgacattgtgaacatgcgtgcagccatcccgtccgacgtttctccacctcgatggcacggccacgcaccaaaagcgagatcctgctgtaaggaagcagacgtattccttggctgcacagtgagcactgtcccccatgcactgctggcgtgcaccctatgtttaaaggagaacgaacacacaaacaggcatcttccgagcactccgctgttctgcactggagggggcggggcgcgaaaaaaaaagagaaaagcgtctcagtgactgacgtatgtcttcgcttttcctttctggatggtgccagcgttggctgccacctatctgccccttgtttcgcaaatggggcattgctttcctgaacctcctcgtccctccgtgtgtatgcatcggcgtgtttgtgtatgggcatgtatgcatatctgggctcctgagtcacggttcgcttctcttcgtgctctctctctactcgtatccattggtgtgtgcgtctccgccacgtagaggtgctgtgtggcgttattcggctcgtgtgtttgtgtgtggtgtgctaggttttgcttccgcggtgcgtccgtcacaacttcatccctcatcctcccttctaccactt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z