BBa_K1955004 1 BBa_K1955004 Ovalbumin 2016-10-13T11:00:00Z 2016-10-17T07:08:03Z The whole sequence of Ovalbumin (OVA) is acquired from GenBank (Accession# AH002466.2). The complete coding sequence of Ovalbumin (OVA) acquired from GenBank (Accession# AH002466.2) . OVA has been commonly used as the antigen for testing the efficiency of antibody response and T cell activation in previous immunology experiments, thus is a easy to use antigen. false false _2422_ 30462 30553 9 false The Ovalbumin (OVA) sequence is a 2098 bp DNA sequence acquired from GenBank (Accession# AH002466.2). We changed several nucleotides of the sequence to avoid the restriction sites and separate the sequence into two parts since it???s too long to synthesis. The restriction site between these two parts is SapI. After cutting the sequence with EcoRI, SapI and PstI, we ligated the two parts with pSB1C3 and confirmed the OVA sequence is correct by DNA sequencing. In order to compare the protein expression efficiency of our expression vector and the original pX63HYG shuttle vector, we added BamHI restriction site at the head and tail of OVA coding sequence for the ligation of pX63HYG shuttle vector and OVA sequence. false Justine Hsu BBa_K1955002 1 BBa_K1955002 Leishmania- 3 prime UTR 2016-10-13T11:00:00Z 2016-10-17T07:14:28Z The Leish-3'UTR sequence originally came from Leishmania genome and is acquired from GenBank. The specific terminator function in Leishmania genome has not been elucidated yet. We selected the 3??? untranslated region of a highly expressed gene, NAGT, to be the transcription terminator and other extra function modifications needed for Leishmania protein expression termination. This biobrick needs to use with the Leish-5'UTR-HYG that we provide as the regulation of protein expression and also the drug selection system for the Leishmania to perform a complete protein expression system in Leishamnia. Add the protein sequence in between the Leish-5'UTR-HYG and Leish-3'UTR for Leishmania to express your target protein. false false _2422_ 30462 30553 9 false The Leish-3???UTR is a 774 bp DNA sequence, which is directly synthesized by IDT. We acquired the original leishmania genome sequence of NAGT coding region and intergenic region from NCBI (GenBank accession# L11705), and choose the 3???UTR region of the sequence as the transcription terminator and protein expression regulator in our expression vector. Some nucleotides had been changed to avoid the restriction site. The function of the 3???UTR intergenic region was studied and published by Dr. Kwang-Poo Chang (Gene 196 (1997) 49???59). false Justine Hsu BBa_K1955006 1 BBa_K1955006 Leishmania 5 prime UTR-HYG-OVA-3 prime UTR 2016-10-13T11:00:00Z 2016-10-19T08:21:44Z Composite of the Leishmania 5 prime UTR-HYG, ovalbumin and 3 prime UTR. The Leish-5'UTR and 3'UTR sequence originally came from Leishmania genome and is acquired from GenBank. The Hygromycin resistant gene and ovalbumin sequences are acquired from GenBank. OVA has been commonly used as the antigen for testing the efficiency of antibody response and T cell activation in previous immunology experiments, thus is a easy to use antigen. We add the OVA coding region in between the Leish-5'UTR-HYG and Leish-3'UTR for leishmania to expression our target protein, OVA. false false _2422_ 30427 30553 9 false The detailed design of the sequence is documented in each part. false Justine Hsu component2523910 1 BBa_K1955004 component2523911 1 BBa_K1955002 component2523909 1 BBa_K1955003 annotation2523910 1 BBa_K1955004 range2523910 1 1455 3567 annotation2523909 1 BBa_K1955003 range2523909 1 1 1446 annotation2523911 1 BBa_K1955002 range2523911 1 3576 4349 BBa_K1955003 1 BBa_K1955003 Leishmania-5 prime UTR-Hygromycin 2016-10-13T11:00:00Z 2016-10-19T03:32:18Z The whole sequence of Hemagglutinin (HA) from H1N1 is acquired from GenBank. The coding sequence of Hemagglutinin (HA) from H1N1 acquired from NCBI. HA is a glycoprotein found on the surface of influenza viruses and can be used in immunological experiments as the target antigen. false false _2422_ 30427 30553 9 false The hemagglutinin (HA) part is a 1700 bp DNA sequence acquired from GenBank (Accession# J02176.1). Some nucleotides of HA sequence had been changed to avoid the restriction sites. In order to compare the protein expression efficiency of our expression vector and the original pX63HYG shuttle vector, we add BamHI restriction site at the head and tail of HA coding sequence for the ligation of pX63HYG shuttle vector and HA sequence. false Justine Hsu BBa_K1955006_sequence 1 aaggcgcacgttgcttctcctccctccctcccctctcttgtgtcggcttgccaaaccccattcatggctgctccgttgccggtttctttttctttctcgcccgtccttcaacccccctgtccttctcattttattgttggcaccacccatcggtcacttcctccctcaccctcccaaccactcacacccacccacacacacacgcacttgcatagaacgtcagcgcttcctatcgagacacacactctcaaacacgagtggtccagtcatcacccgcacactccactctcatttttctttcgcagtatcatctctctaatctctcacggaggtatttctcactcgatatacacacgcgtgcataccgcttccctaacccactccacacacacacacacactggcgcacgtcctctgaaaaatgaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggagttcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttcttcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagtactagagcaaggatccacatacagctagaaagctgtattgcctttagcagtcaagctcgaactgttctcctgtttgctcttgacaactcagagttcaccatgggctccatcggtgcagcaagcatggaattttgttttgatgtattcaaggagctcaaagtccaccatgccaatgagaacatcttctactgccccattgccatcatgtcagctctagccatggtatacctgggtgcaaaagacagcaccaggacacaaataaataaggtgagcctacagttaaacatactattattttcaattacaggttgttcgctttgataaacttccaggattcggagacagtattgaagctcaggtacagaaataatttcacctttctttctctttctattcagtgtggcacatctgtaaacgttcactcttcacttagagacatcctcaaccaaatcaccaaaccaaatgatgtttattcgttcagccttgccagtagactttatgctgaagagagatacccaatcctgccagtaagttgctctaaaatctctgaatactcttgctttacaggaatacttgcagtgtgtgaaggaactgtatagaggaggcttggaacctatcaactttcaaacagcagcagatcaagccagagagctcatcaattcctgggtagaaagtcagacaaatggtaaggtagaacatgctttgtttcctattcattcttaaaggaattatcagaaatgtccttcagccaagctccgtggattctcaaactgcaatggttctggttaatgccattgtcttcaaaggactgtgggagaaagcatttaaggatgaagacacacaagcaatgcctttcagagtgactgaggtatatgggcataccttagattttttttttggttctccagcaagaaagcaaacctgtgcagatgatgtaccagattggtttatttagagtggcatcaatggcttctgagaaaatgaagatcctggagcttccatttgccagtgggacaatgagcatgttggtgctgttgcctgatgaagtctcaggccttgagcaggtatggccctagaacttggccttgagagtataatcaactttgaaaaactgactgaatggaccagttctaatgttatggaagagaggaagatcaaagtgtacttacctcgcatgaagatggaggaaaaatacaacctcacatctgtcttaatggctatgggcattactgacgtgtttagctcttcagccaatctgtctggcatctcctcagcagagagcctgaagatatctcaagctgtccatgcagcacatgcagaaatcaatgaagcaggcagagaggtggtagggtcagcagaggctggagtggatgctgcaagcgtctctgaagaatttagggctgaccatccattcctcttctgtatcaagcacatcgcaaccaacgccgttctcttctttggcagatgtgtttccccttaaaaagaagaaagctgaaaaactctgtcccttccaacaagacccagagcactgtagtatcaggggtaaaatgaaaagtatgttatctgctgcatccagacttcataaaagctggagcttaatcaagaaaaaaaatcagaaagaaattacactgtgagaacaggtgcaattcacttttcctttacacagagtaatactggtaactcatggatgaaggcttaagggaatgaaattggagtcatcacactgaaaaatgcaacctgatacatcagcagaaggtttatgggggaaaaatgcagccttccaattaagccagatatctgtatgaccaagctgctccagaattagtcactcaaaatctctcagattaaattatcaactgtcaccaaccattcctatgctgacaaggcaattgcttgttctctgtgttcctgatactacaaggctcatcctgacttcctaaagatgcattataaaaatcttataattcacatttctccctaaactttgactcaatcatggtatgttggcaaatatggtatattactattcaaattgttttccttgtacccatatgtaatgggtcttgtgaatgtgctcttttgttcctttaatcataataaaaacatgtttaagcggatcctactagagagccaggtttgaaggacgcgcaagagaggccatgcactgtgctgttgagtttttgttgctgttttcttgttttcacccctgtgtgtgcgggtgtcgattgcccttgaatgcgcacattgcgctccgagaaggctcgccgatggggtgaaacgaggactgctgacgctgacattgtgaacatgcgtgcagccatcccgtccgacgtttctccacctcgatggcacggccacgcaccaaaagcgagatcctgctgtaaggaagcagacgtattccttggctgcacagtgagcactgtcccccatgcactgctggcgtgcaccctatgtttaaaggagaacgaacacacaaacaggcatcttccgagcactccgctgttctgcactggagggggcggggcgcgaaaaaaaaagagaaaagcgtctcagtgactgacgtatgtcttcgcttttcctttctggatggtgccagcgttggctgccacctatctgccccttgtttcgcaaatggggcattgctttcctgaacctcctcgtccctccgtgtgtatgcatcggcgtgtttgtgtatgggcatgtatgcatatctgggctcctgagtcacggttcgcttctcttcgtgctctctctctactcgtatccattggtgtgtgcgtctccgccacgtagaggtgctgtgtggcgttattcggctcgtgtgtttgtgtgtggtgtgctaggttttgcttccgcggtgcgtccgtcacaacttcatccctcatcctcccttctaccactt BBa_K1955003_sequence 1 aaggcgcacgttgcttctcctccctccctcccctctcttgtgtcggcttgccaaaccccattcatggctgctccgttgccggtttctttttctttctcgcccgtccttcaacccccctgtccttctcattttattgttggcaccacccatcggtcacttcctccctcaccctcccaaccactcacacccacccacacacacacgcacttgcatagaacgtcagcgcttcctatcgagacacacactctcaaacacgagtggtccagtcatcacccgcacactccactctcatttttctttcgcagtatcatctctctaatctctcacggaggtatttctcactcgatatacacacgcgtgcataccgcttccctaacccactccacacacacacacacactggcgcacgtcctctgaaaaatgaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggagttcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttcttcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatag BBa_K1955004_sequence 1 caaggatccacatacagctagaaagctgtattgcctttagcagtcaagctcgaactgttctcctgtttgctcttgacaactcagagttcaccatgggctccatcggtgcagcaagcatggaattttgttttgatgtattcaaggagctcaaagtccaccatgccaatgagaacatcttctactgccccattgccatcatgtcagctctagccatggtatacctgggtgcaaaagacagcaccaggacacaaataaataaggtgagcctacagttaaacatactattattttcaattacaggttgttcgctttgataaacttccaggattcggagacagtattgaagctcaggtacagaaataatttcacctttctttctctttctattcagtgtggcacatctgtaaacgttcactcttcacttagagacatcctcaaccaaatcaccaaaccaaatgatgtttattcgttcagccttgccagtagactttatgctgaagagagatacccaatcctgccagtaagttgctctaaaatctctgaatactcttgctttacaggaatacttgcagtgtgtgaaggaactgtatagaggaggcttggaacctatcaactttcaaacagcagcagatcaagccagagagctcatcaattcctgggtagaaagtcagacaaatggtaaggtagaacatgctttgtttcctattcattcttaaaggaattatcagaaatgtccttcagccaagctccgtggattctcaaactgcaatggttctggttaatgccattgtcttcaaaggactgtgggagaaagcatttaaggatgaagacacacaagcaatgcctttcagagtgactgaggtatatgggcataccttagattttttttttggttctccagcaagaaagcaaacctgtgcagatgatgtaccagattggtttatttagagtggcatcaatggcttctgagaaaatgaagatcctggagcttccatttgccagtgggacaatgagcatgttggtgctgttgcctgatgaagtctcaggccttgagcaggtatggccctagaacttggccttgagagtataatcaactttgaaaaactgactgaatggaccagttctaatgttatggaagagaggaagatcaaagtgtacttacctcgcatgaagatggaggaaaaatacaacctcacatctgtcttaatggctatgggcattactgacgtgtttagctcttcagccaatctgtctggcatctcctcagcagagagcctgaagatatctcaagctgtccatgcagcacatgcagaaatcaatgaagcaggcagagaggtggtagggtcagcagaggctggagtggatgctgcaagcgtctctgaagaatttagggctgaccatccattcctcttctgtatcaagcacatcgcaaccaacgccgttctcttctttggcagatgtgtttccccttaaaaagaagaaagctgaaaaactctgtcccttccaacaagacccagagcactgtagtatcaggggtaaaatgaaaagtatgttatctgctgcatccagacttcataaaagctggagcttaatcaagaaaaaaaatcagaaagaaattacactgtgagaacaggtgcaattcacttttcctttacacagagtaatactggtaactcatggatgaaggcttaagggaatgaaattggagtcatcacactgaaaaatgcaacctgatacatcagcagaaggtttatgggggaaaaatgcagccttccaattaagccagatatctgtatgaccaagctgctccagaattagtcactcaaaatctctcagattaaattatcaactgtcaccaaccattcctatgctgacaaggcaattgcttgttctctgtgttcctgatactacaaggctcatcctgacttcctaaagatgcattataaaaatcttataattcacatttctccctaaactttgactcaatcatggtatgttggcaaatatggtatattactattcaaattgttttccttgtacccatatgtaatgggtcttgtgaatgtgctcttttgttcctttaatcataataaaaacatgtttaagcggatcc BBa_K1955002_sequence 1 agccaggtttgaaggacgcgcaagagaggccatgcactgtgctgttgagtttttgttgctgttttcttgttttcacccctgtgtgtgcgggtgtcgattgcccttgaatgcgcacattgcgctccgagaaggctcgccgatggggtgaaacgaggactgctgacgctgacattgtgaacatgcgtgcagccatcccgtccgacgtttctccacctcgatggcacggccacgcaccaaaagcgagatcctgctgtaaggaagcagacgtattccttggctgcacagtgagcactgtcccccatgcactgctggcgtgcaccctatgtttaaaggagaacgaacacacaaacaggcatcttccgagcactccgctgttctgcactggagggggcggggcgcgaaaaaaaaagagaaaagcgtctcagtgactgacgtatgtcttcgcttttcctttctggatggtgccagcgttggctgccacctatctgccccttgtttcgcaaatggggcattgctttcctgaacctcctcgtccctccgtgtgtatgcatcggcgtgtttgtgtatgggcatgtatgcatatctgggctcctgagtcacggttcgcttctcttcgtgctctctctctactcgtatccattggtgtgtgcgtctccgccacgtagaggtgctgtgtggcgttattcggctcgtgtgtttgtgtgtggtgtgctaggttttgcttccgcggtgcgtccgtcacaacttcatccctcatcctcccttctaccactt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z