LOCUS       BBa_K1955007            2954 bp    DNA     linear       06-Dec-2025
DEFINITION  E.coli-Leishmania shuttle vector HYG-GFP
ACCESSION   BBa_K1955007
VERSION     BBa_K1955007.1  
FEATURES             Location/Qualifiers
     promoter        1..1446
                     /label=BBa_K1955003
     CDS             1453..2172
                     /label=GFP protein
     CDS             1453..2172
                     /label=BBa_E0040
     terminator      2181..2954
                     /label=BBa_K1955002
ORIGIN
        1 aaggcgcacg ttgcttctcc tccctccctc ccctctcttg tgtcggcttg ccaaacccca
       61 ttcatggctg ctccgttgcc ggtttctttt tctttctcgc ccgtccttca acccccctgt
      121 ccttctcatt ttattgttgg caccacccat cggtcacttc ctccctcacc ctcccaacca
      181 ctcacaccca cccacacaca cacgcacttg catagaacgt cagcgcttcc tatcgagaca
      241 cacactctca aacacgagtg gtccagtcat cacccgcaca ctccactctc atttttcttt
      301 cgcagtatca tctctctaat ctctcacgga ggtatttctc actcgatata cacacgcgtg
      361 cataccgctt ccctaaccca ctccacacac acacacacac tggcgcacgt cctctgaaaa
      421 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
      481 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
      541 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
      601 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
      661 ggggagttca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
      721 caagacctgc ctgaaaccga actgcccgct gttcttcagc cggtcgcgga ggccatggat
      781 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
      841 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
      901 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
      961 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     1021 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     1081 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     1141 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     1201 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     1261 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     1321 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     1381 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     1441 gaatagtact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
     1501 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
     1561 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
     1621 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
     1681 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
     1741 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
     1801 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
     1861 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
     1921 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
     1981 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     2041 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     2101 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     2161 tacaaataat aatactagag agccaggttt gaaggacgcg caagagaggc catgcactgt
     2221 gctgttgagt ttttgttgct gttttcttgt tttcacccct gtgtgtgcgg gtgtcgattg
     2281 cccttgaatg cgcacattgc gctccgagaa ggctcgccga tggggtgaaa cgaggactgc
     2341 tgacgctgac attgtgaaca tgcgtgcagc catcccgtcc gacgtttctc cacctcgatg
     2401 gcacggccac gcaccaaaag cgagatcctg ctgtaaggaa gcagacgtat tccttggctg
     2461 cacagtgagc actgtccccc atgcactgct ggcgtgcacc ctatgtttaa aggagaacga
     2521 acacacaaac aggcatcttc cgagcactcc gctgttctgc actggagggg gcggggcgcg
     2581 aaaaaaaaag agaaaagcgt ctcagtgact gacgtatgtc ttcgcttttc ctttctggat
     2641 ggtgccagcg ttggctgcca cctatctgcc ccttgtttcg caaatggggc attgctttcc
     2701 tgaacctcct cgtccctccg tgtgtatgca tcggcgtgtt tgtgtatggg catgtatgca
     2761 tatctgggct cctgagtcac ggttcgcttc tcttcgtgct ctctctctac tcgtatccat
     2821 tggtgtgtgc gtctccgcca cgtagaggtg ctgtgtggcg ttattcggct cgtgtgtttg
     2881 tgtgtggtgt gctaggtttt gcttccgcgg tgcgtccgtc acaacttcat ccctcatcct
     2941 cccttctacc actt
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