BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_R0011
1
lacI+pL
Promoter (lacI regulated, lambda pL hybrid)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
represillator of Elowitz and Leibler (2000)
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
false
true
_1_
0
24
7
In stock
false
<P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
true
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
annotation1999
1
lac O1
range1999
1
3
19
annotation7064
1
BBa_R0011
range7064
1
1
54
annotation2000
1
-35
range2000
1
20
25
annotation2001
1
lac O1
range2001
1
26
42
annotation2002
1
-10
range2002
1
43
48
BBa_K1956003
1
BBa_K1956003
Lambda pL hybrid with B0032
2016-10-13T11:00:00Z
2016-10-14T08:01:34Z
Cloning Biobricks
Lambda pL hybrid with B0032
false
false
_2423_
24882
24882
9
false
NaN
false
Nitish Kumar Singh
component2505684
1
BBa_R0011
component2505690
1
BBa_B0032
annotation2505684
1
BBa_R0011
range2505684
1
1
54
annotation2505690
1
BBa_B0032
range2505690
1
64
76
BBa_B0032_sequence
1
tcacacaggaaag
BBa_K1956003_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaag
BBa_R0011_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z