LOCUS       BBa_K1956013             302 bp    DNA     linear       15-Apr-2025
DEFINITION  gRNA for Kanamycin resistance gene under lambda pL hybrid promoter 
            with terminator
ACCESSION   BBa_K1956013
VERSION     BBa_K1956013.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    64..165
                     /label=BBa_K1956045
     misc_feature    1..165
                     /label=BBa_K1956010
     stem_loop       185..228
                     /label=stem_loop
     misc_feature    174..253
                     /label=BBa_B0010
     terminator      174..253
                     /label=BBa_B0010
     stem_loop       269..288
                     /label=T7 TE
     misc_feature    295..295
                     /label=stop
     polyA_site      289..302
                     /label=polya
     misc_feature    262..302
                     /label=BBa_B0012
     terminator      262..302
                     /label=BBa_B0012
     terminator      174..302
                     /label=BBa_B0015
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagagaaaca actctggcgc atcgttttag agctagaaat agcaagttaa aataaggcta
      121 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttacta gagccaggca
      181 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      241 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      301 ta
//