LOCUS       BBa_K1956019            2132 bp    DNA     linear       23-Dec-2024
DEFINITION  RIBOSON with plasmid control
ACCESSION   BBa_K1956019
VERSION     BBa_K1956019.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    44..192
                     /label=BBa_K1956040
     CDS             199..918
                     /label=GFP protein
     CDS             199..918
                     /label=BBa_E0040
     stem_loop       938..981
                     /label=stem_loop
     misc_feature    927..1006
                     /label=BBa_B0010
     terminator      927..1006
                     /label=BBa_B0010
     stem_loop       1022..1041
                     /label=T7 TE
     misc_feature    1048..1048
                     /label=stop
     polyA_site      1042..1055
                     /label=polya
     misc_feature    1015..1055
                     /label=BBa_B0012
     terminator      1015..1055
                     /label=BBa_B0012
     misc_feature    199..1055
                     /label=BBa_I13401
     CDS             1064..1151
                     /label=end of LacI coding region (inactive)
     misc_feature    1064..1263
                     /label=BBa_R0010
     misc_feature    1152..1189
                     /label=CAP binding site
     promoter        1200..1205
                     /label=-35
     promoter        1224..1229
                     /label=-10
     misc_feature    1229..1263
                     /label=LacI binding site
     misc_feature    1236..1236
                     /label=start
     promoter        1064..1263
                     /label=BBa_R0010
     misc_feature    1276..1279
                     /label=conserved
     RBS             1272..1283
                     /label=BBa_B0034
     CDS             1290..1964
                     /label=mrfp1
     misc_feature    1971..1995
                     /label=Help:Barcodes
     CDS             1290..1970
                     /label=BBa_E1010
     stem_loop       2015..2058
                     /label=stem_loop
     misc_feature    2004..2083
                     /label=BBa_B0010
     terminator      2004..2083
                     /label=BBa_B0010
     stem_loop       2099..2118
                     /label=T7 TE
     misc_feature    2125..2125
                     /label=stop
     polyA_site      2119..2132
                     /label=polya
     misc_feature    2092..2132
                     /label=BBa_B0012
     terminator      2092..2132
                     /label=BBa_B0012
     misc_feature    1064..2132
                     /label=BBa_J04450
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagtaggaaa aaggtaccca
       61 ggccgctaaa ctgtaaggag gttaccagtt tagcggtctg ggtaccttcg tacggacgac
      121 cttcaccagc tattaatgta acctccttac agtgtagcgg tctgggtacc taccgctata
      181 ctgtaaggag gttactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
      241 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      301 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      361 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      421 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      481 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      541 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      601 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      661 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      721 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      781 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      841 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      901 gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      961 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1021 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagcaatacg caaaccgcct
     1081 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     1141 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     1201 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     1261 acatactaga gaaagaggag aaatactaga tggcttcctc cgaagacgtt atcaaagagt
     1321 tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag
     1381 gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag
     1441 gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag
     1501 cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt
     1561 tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact
     1621 cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt
     1681 ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt
     1741 acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg
     1801 gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg
     1861 gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg
     1921 ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt
     1981 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     2041 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     2101 ctcaccttcg ggtgggcctt tctgcgttta ta
//