##gff-version 3
##sequence-region BBa_K1956019 1 2132
BBa_K1956019	.	engineered_region	1064	2132	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_K1956019	.	ribosome_entry_site	1272	1283	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1956019	.	conserved_region	1276	1279	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1956019	.	promoter	1064	1263	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1956019	.	CDS	1064	1151	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1956019	.	promoter	1224	1229	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1956019	.	non_covalent_binding_site	1229	1263	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1956019	.	engineered_region	1064	1263	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1956019	.	promoter	1200	1205	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1956019	.	non_covalent_binding_site	1152	1189	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1956019	.	start_codon	1236	1236	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1956019	.	CDS	1290	1970	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1956019	.	CDS	1290	1964	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1956019	.	engineered_tag	1971	1995	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1956019	.	terminator	2004	2083	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1956019	.	stem_loop	2015	2058	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1956019	.	engineered_region	2004	2083	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1956019	.	terminator	2092	2132	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1956019	.	stem_loop	2099	2118	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1956019	.	polyA_site	2119	2132	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1956019	.	stop_codon	2125	2125	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1956019	.	engineered_region	2092	2132	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1956019	.	sequence_feature	44	192	.	+	0	ID=BBa_K1956040;Name=BBa_K1956040
BBa_K1956019	.	engineered_region	199	1055	.	+	0	ID=BBa_I13401;Name=BBa_I13401
BBa_K1956019	.	terminator	927	1006	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13401
BBa_K1956019	.	stem_loop	938	981	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1956019	.	engineered_region	927	1006	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1956019	.	CDS	199	918	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13401
BBa_K1956019	.	CDS	199	918	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1956019	.	terminator	1015	1055	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13401
BBa_K1956019	.	stem_loop	1022	1041	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1956019	.	polyA_site	1042	1055	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1956019	.	stop_codon	1048	1048	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1956019	.	engineered_region	1015	1055	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1956019	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
>BBa_K1956019
ttgacggctagctcagtcctaggtacagtgctagctactagagtaggaaaaaggtacccaggccgctaaactgtaagga
ggttaccagtttagcggtctgggtaccttcgtacggacgaccttcaccagctattaatgtaacctccttacagtgtagc
ggtctgggtacctaccgctatactgtaaggaggttactagatgcgtaaaggagaagaacttttcactggagttgtccca
attcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacg
gaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggtta
tggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttat
gtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgataccc
ttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaacta
taactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacatt
gaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagaca
accattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgt
aacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcatta
atgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcat
taggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacat
actagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatg
gaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgcta
aactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagc
ttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatg
aacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaac
tgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtat
gtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaa
gttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacct
cccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctg
atagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata