LOCUS       BBa_K1956020            2137 bp    DNA     linear       17-May-2024
DEFINITION  RIBOSOFF with plasmid control
ACCESSION   BBa_K1956020
VERSION     BBa_K1956020.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     misc_feature    44..197
                     /label=BBa_K1956037
     promoter        1..197
                     /label=BBa_K1956005
     CDS             204..923
                     /label=GFP protein
     CDS             204..923
                     /label=BBa_E0040
     stem_loop       943..986
                     /label=stem_loop
     misc_feature    932..1011
                     /label=BBa_B0010
     terminator      932..1011
                     /label=BBa_B0010
     stem_loop       1027..1046
                     /label=T7 TE
     misc_feature    1053..1053
                     /label=stop
     polyA_site      1047..1060
                     /label=polya
     misc_feature    1020..1060
                     /label=BBa_B0012
     terminator      1020..1060
                     /label=BBa_B0012
     misc_feature    204..1060
                     /label=BBa_I13401
     CDS             1069..1156
                     /label=end of LacI coding region (inactive)
     misc_feature    1069..1268
                     /label=BBa_R0010
     misc_feature    1157..1194
                     /label=CAP binding site
     promoter        1205..1210
                     /label=-35
     promoter        1229..1234
                     /label=-10
     misc_feature    1234..1268
                     /label=LacI binding site
     misc_feature    1241..1241
                     /label=start
     promoter        1069..1268
                     /label=BBa_R0010
     misc_feature    1281..1284
                     /label=conserved
     RBS             1277..1288
                     /label=BBa_B0034
     CDS             1295..1969
                     /label=mrfp1
     misc_feature    1976..2000
                     /label=Help:Barcodes
     CDS             1295..1975
                     /label=BBa_E1010
     stem_loop       2020..2063
                     /label=stem_loop
     misc_feature    2009..2088
                     /label=BBa_B0010
     terminator      2009..2088
                     /label=BBa_B0010
     stem_loop       2104..2123
                     /label=T7 TE
     misc_feature    2130..2130
                     /label=stop
     polyA_site      2124..2137
                     /label=polya
     misc_feature    2097..2137
                     /label=BBa_B0012
     terminator      2097..2137
                     /label=BBa_B0012
     misc_feature    1069..2137
                     /label=BBa_J04450
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaaaaag ggcgtggagc
       61 gtctggtcgc atcgctctaa gaatgcgacc agatgctcca cgcccagtcg cgtagcgtca
      121 aaaaaaaaaa gagcgatgcg gccagatggg gggagggcta gtaccctcct cccatctggt
      181 cgcatagagg aggagggtac tagatgcgta aaggagaaga acttttcact ggagttgtcc
      241 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
      301 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
      361 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga
      421 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
      481 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
      541 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
      601 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
      661 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
      721 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
      781 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
      841 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
      901 tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag
      961 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1021 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagca atacgcaaac
     1081 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     1141 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     1201 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     1261 ttcacacata ctagagaaag aggagaaata ctagatggct tcctccgaag acgttatcaa
     1321 agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat
     1381 cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac
     1441 caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc
     1501 caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga
     1561 aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca
     1621 ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt
     1681 cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg
     1741 tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga
     1801 cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac cggttcagct
     1861 gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac
     1921 catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt aataacgctg
     1981 atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     2041 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     2101 actggctcac cttcgggtgg gcctttctgc gtttata
//