LOCUS       BBa_K1956021            2145 bp    DNA     linear       26-Dec-2024
DEFINITION  RIBOSOFF with plasmid control
ACCESSION   BBa_K1956021
VERSION     BBa_K1956021.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     misc_feature    44..205
                     /label=BBa_K1956038
     CDS             212..931
                     /label=GFP protein
     CDS             212..931
                     /label=BBa_E0040
     stem_loop       951..994
                     /label=stem_loop
     misc_feature    940..1019
                     /label=BBa_B0010
     terminator      940..1019
                     /label=BBa_B0010
     stem_loop       1035..1054
                     /label=T7 TE
     misc_feature    1061..1061
                     /label=stop
     polyA_site      1055..1068
                     /label=polya
     misc_feature    1028..1068
                     /label=BBa_B0012
     terminator      1028..1068
                     /label=BBa_B0012
     misc_feature    212..1068
                     /label=BBa_I13401
     CDS             1077..1164
                     /label=end of LacI coding region (inactive)
     misc_feature    1077..1276
                     /label=BBa_R0010
     misc_feature    1165..1202
                     /label=CAP binding site
     promoter        1213..1218
                     /label=-35
     promoter        1237..1242
                     /label=-10
     misc_feature    1242..1276
                     /label=LacI binding site
     misc_feature    1249..1249
                     /label=start
     promoter        1077..1276
                     /label=BBa_R0010
     misc_feature    1289..1292
                     /label=conserved
     RBS             1285..1296
                     /label=BBa_B0034
     CDS             1303..1977
                     /label=mrfp1
     misc_feature    1984..2008
                     /label=Help:Barcodes
     CDS             1303..1983
                     /label=BBa_E1010
     stem_loop       2028..2071
                     /label=stem_loop
     misc_feature    2017..2096
                     /label=BBa_B0010
     terminator      2017..2096
                     /label=BBa_B0010
     stem_loop       2112..2131
                     /label=T7 TE
     misc_feature    2138..2138
                     /label=stop
     polyA_site      2132..2145
                     /label=polya
     misc_feature    2105..2145
                     /label=BBa_B0012
     terminator      2105..2145
                     /label=BBa_B0012
     misc_feature    1077..2145
                     /label=BBa_J04450
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaaaatg ggcgtggagc
       61 gtctggtcgc atcgctctga ggaagaatgc gaccagatgc tccacgccca gtcgcgtacc
      121 gtcaaaaaaa aaacctcaga gcgatgcggc cagatggggg gagggctagt accctcctcc
      181 catctggtcg catagaggag gagggtacta gatgcgtaaa ggagaagaac ttttcactgg
      241 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
      301 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
      361 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg
      421 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga
      481 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
      541 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt
      601 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt
      661 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa
      721 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
      781 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
      841 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
      901 acatggcatg gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa
      961 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1021 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagcaat
     1081 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     1141 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     1201 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     1261 ataacaattt cacacatact agagaaagag gagaaatact agatggcttc ctccgaagac
     1321 gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag
     1381 ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg
     1441 aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag
     1501 tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc
     1561 ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc
     1621 gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt
     1681 accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc
     1741 accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa
     1801 ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg
     1861 gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa
     1921 gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa
     1981 taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg
     2041 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     2101 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//