LOCUS       BBa_K1956022            2018 bp    DNA     linear       17-Dec-2024
DEFINITION  Noise in Device measurement
ACCESSION   BBa_K1956022
VERSION     BBa_K1956022.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             64..78
                     /label=RBS-1\Strong
     RBS             71..75
                     /label=RBS
     misc_feature    64..78
                     /label=BBa_B0030
     RBS             64..78
                     /label=BBa_B0030
     CDS             85..804
                     /label=GFP protein
     CDS             85..804
                     /label=BBa_E0040
     stem_loop       824..867
                     /label=stem_loop
     misc_feature    813..892
                     /label=BBa_B0010
     terminator      813..892
                     /label=BBa_B0010
     stem_loop       908..927
                     /label=T7 TE
     misc_feature    934..934
                     /label=stop
     polyA_site      928..941
                     /label=polya
     misc_feature    901..941
                     /label=BBa_B0012
     terminator      901..941
                     /label=BBa_B0012
     misc_feature    85..941
                     /label=BBa_I13401
     CDS             950..1037
                     /label=end of LacI coding region (inactive)
     misc_feature    950..1149
                     /label=BBa_R0010
     misc_feature    1038..1075
                     /label=CAP binding site
     promoter        1086..1091
                     /label=-35
     promoter        1110..1115
                     /label=-10
     misc_feature    1115..1149
                     /label=LacI binding site
     misc_feature    1122..1122
                     /label=start
     promoter        950..1149
                     /label=BBa_R0010
     misc_feature    1162..1165
                     /label=conserved
     RBS             1158..1169
                     /label=BBa_B0034
     CDS             1176..1850
                     /label=mrfp1
     misc_feature    1857..1881
                     /label=Help:Barcodes
     CDS             1176..1856
                     /label=BBa_E1010
     stem_loop       1901..1944
                     /label=stem_loop
     misc_feature    1890..1969
                     /label=BBa_B0010
     terminator      1890..1969
                     /label=BBa_B0010
     stem_loop       1985..2004
                     /label=T7 TE
     misc_feature    2011..2011
                     /label=stop
     polyA_site      2005..2018
                     /label=polya
     misc_feature    1978..2018
                     /label=BBa_B0012
     terminator      1978..2018
                     /label=BBa_B0012
     misc_feature    950..2018
                     /label=BBa_J04450
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagattaaag aggagaaata ctagatgcgt aaaggagaag aacttttcac tggagttgtc
      121 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
      181 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
      241 ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg
      301 agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat
      361 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc
      421 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa
      481 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc
      541 atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa
      601 gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct
      661 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac
      721 gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc
      781 atggatgaac tatacaaata ataatactag agccaggcat caaataaaac gaaaggctca
      841 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      901 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagc aatacgcaaa
      961 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     1021 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     1081 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     1141 tttcacacat actagagaaa gaggagaaat actagatggc ttcctccgaa gacgttatca
     1201 aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa
     1261 tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta
     1321 ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt
     1381 ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg
     1441 aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc
     1501 aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact
     1561 tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac
     1621 gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag
     1681 acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc
     1741 tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca
     1801 ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct
     1861 gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc
     1921 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1981 cactggctca ccttcgggtg ggcctttctg cgtttata
//