##gff-version 3
##sequence-region BBa_K1956022 1 2018
BBa_K1956022	.	engineered_region	950	2018	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_K1956022	.	CDS	1176	1856	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1956022	.	engineered_tag	1857	1881	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1956022	.	CDS	1176	1850	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1956022	.	terminator	1890	1969	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1956022	.	engineered_region	1890	1969	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1956022	.	stem_loop	1901	1944	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1956022	.	terminator	1978	2018	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1956022	.	stop_codon	2011	2011	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1956022	.	polyA_site	2005	2018	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1956022	.	stem_loop	1985	2004	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1956022	.	engineered_region	1978	2018	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1956022	.	promoter	950	1149	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1956022	.	non_covalent_binding_site	1038	1075	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1956022	.	non_covalent_binding_site	1115	1149	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1956022	.	promoter	1086	1091	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1956022	.	engineered_region	950	1149	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1956022	.	promoter	1110	1115	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1956022	.	start_codon	1122	1122	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1956022	.	CDS	950	1037	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1956022	.	ribosome_entry_site	1158	1169	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1956022	.	conserved_region	1162	1165	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1956022	.	engineered_region	85	941	.	+	0	ID=BBa_I13401;Name=BBa_I13401
BBa_K1956022	.	terminator	901	941	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13401
BBa_K1956022	.	stop_codon	934	934	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1956022	.	polyA_site	928	941	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1956022	.	stem_loop	908	927	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1956022	.	engineered_region	901	941	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1956022	.	CDS	85	804	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13401
BBa_K1956022	.	CDS	85	804	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1956022	.	terminator	813	892	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13401
BBa_K1956022	.	engineered_region	813	892	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1956022	.	stem_loop	824	867	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1956022	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K1956022	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1956022	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1956022	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1956022	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1956022	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1956022	.	ribosome_entry_site	64	78	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1956022	.	ribosome_entry_site	71	75	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1956022	.	engineered_region	64	78	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1956022	.	ribosome_entry_site	64	78	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
>BBa_K1956022
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagattaaagaggagaaat
actagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggca
caaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactgga
aaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcata
tgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgg
gaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgatttt
aaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaac
aaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatca
acaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaa
gatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaac
tatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactaga
gcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaag
cgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggc
tcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcct
ccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcga
aggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttc
gcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacc
tgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttaccca
ggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggtt
atgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatca
aaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttca
gctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtac
gaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggca
tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag
agtcacactggctcaccttcgggtgggcctttctgcgtttata