##gff-version 3
##sequence-region BBa_K1956023 1 2017
BBa_K1956023	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K1956023	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1956023	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1956023	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1956023	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1956023	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1956023	.	ribosome_entry_site	64	77	.	+	0	ID=BBa_B0031;Name=BBa_B0031
BBa_K1956023	.	conserved_region	70	73	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K1956023	.	engineered_region	84	940	.	+	0	ID=BBa_I13401;Name=BBa_I13401
BBa_K1956023	.	CDS	84	803	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13401
BBa_K1956023	.	CDS	84	803	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1956023	.	terminator	812	891	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13401
BBa_K1956023	.	stem_loop	823	866	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1956023	.	engineered_region	812	891	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1956023	.	terminator	900	940	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13401
BBa_K1956023	.	stem_loop	907	926	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1956023	.	stop_codon	933	933	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1956023	.	polyA_site	927	940	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1956023	.	engineered_region	900	940	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1956023	.	engineered_region	949	2017	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_K1956023	.	promoter	949	1148	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1956023	.	non_covalent_binding_site	1037	1074	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1956023	.	promoter	1109	1114	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1956023	.	non_covalent_binding_site	1114	1148	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1956023	.	promoter	1085	1090	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1956023	.	start_codon	1121	1121	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1956023	.	engineered_region	949	1148	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1956023	.	CDS	949	1036	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1956023	.	ribosome_entry_site	1157	1168	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1956023	.	conserved_region	1161	1164	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1956023	.	CDS	1175	1855	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1956023	.	engineered_tag	1856	1880	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1956023	.	CDS	1175	1849	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1956023	.	terminator	1889	1968	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1956023	.	stem_loop	1900	1943	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1956023	.	engineered_region	1889	1968	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1956023	.	terminator	1977	2017	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1956023	.	stem_loop	1984	2003	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1956023	.	stop_codon	2010	2010	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1956023	.	polyA_site	2004	2017	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1956023	.	engineered_region	1977	2017	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K1956023
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaaccta
ctagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcac
aaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaa
aactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatat
gaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacggg
aactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgatttta
aagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaaca
aaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaa
caaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaag
atcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaact
atacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctc
cgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaa
ggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcg
cttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacct
gaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag
gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggtta
tgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaa
aatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcag
ctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacg
aacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttata