LOCUS BBa_K1956024 2016 bp DNA linear 26-Nov-2024 DEFINITION Noise in Device measurement ACCESSION BBa_K1956024 VERSION BBa_K1956024.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 RBS 64..76 /label=RBS-3\Weak RBS 70..73 /label=RBS misc_feature 64..76 /label=BBa_B0032 RBS 64..76 /label=BBa_B0032 CDS 83..802 /label=GFP protein CDS 83..802 /label=BBa_E0040 stem_loop 822..865 /label=stem_loop misc_feature 811..890 /label=BBa_B0010 terminator 811..890 /label=BBa_B0010 stem_loop 906..925 /label=T7 TE misc_feature 932..932 /label=stop polyA_site 926..939 /label=polya misc_feature 899..939 /label=BBa_B0012 terminator 899..939 /label=BBa_B0012 misc_feature 83..939 /label=BBa_I13401 CDS 948..1035 /label=end of LacI coding region (inactive) misc_feature 948..1147 /label=BBa_R0010 misc_feature 1036..1073 /label=CAP binding site promoter 1084..1089 /label=-35 promoter 1108..1113 /label=-10 misc_feature 1113..1147 /label=LacI binding site misc_feature 1120..1120 /label=start promoter 948..1147 /label=BBa_R0010 misc_feature 1160..1163 /label=conserved RBS 1156..1167 /label=BBa_B0034 CDS 1174..1848 /label=mrfp1 misc_feature 1855..1879 /label=Help:Barcodes CDS 1174..1854 /label=BBa_E1010 stem_loop 1899..1942 /label=stem_loop misc_feature 1888..1967 /label=BBa_B0010 terminator 1888..1967 /label=BBa_B0010 stem_loop 1983..2002 /label=T7 TE misc_feature 2009..2009 /label=stop polyA_site 2003..2016 /label=polya misc_feature 1976..2016 /label=BBa_B0012 terminator 1976..2016 /label=BBa_B0012 misc_feature 948..2016 /label=BBa_J04450 misc_feature 1..2016 /label=BBa_K1956014 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagtcacaca ggaaagtact agatgcgtaa aggagaagaa cttttcactg gagttgtccc 121 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg 181 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact 241 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag 301 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt 361 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa 421 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga 481 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat 541 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga 601 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt 661 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga 721 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat 781 ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt 841 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 901 acactggctc accttcgggt gggcctttct gcgtttatat actagagcaa tacgcaaacc 961 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 1021 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca 1081 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt 1141 tcacacatac tagagaaaga ggagaaatac tagatggctt cctccgaaga cgttatcaaa 1201 gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc 1261 gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc 1321 aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc 1381 aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa 1441 ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag 1501 gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc 1561 ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt 1621 atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac 1681 ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg 1741 ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc 1801 atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga 1861 tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga 1921 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 1981 ctggctcacc ttcgggtggg cctttctgcg tttata //