##gff-version 3
##sequence-region BBa_K1956024 1 2016
BBa_K1956024	.	engineered_region	1	2016	.	+	0	ID=BBa_K1956014;Name=BBa_K1956014
BBa_K1956024	.	engineered_region	948	2016	.	+	0	ID=BBa_J04450;Name=BBa_J04450;Parent=BBa_K1956014
BBa_K1956024	.	ribosome_entry_site	1156	1167	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1956024	.	conserved_region	1160	1163	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1956024	.	terminator	1888	1967	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1956024	.	engineered_region	1888	1967	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1956024	.	stem_loop	1899	1942	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1956024	.	terminator	1976	2016	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1956024	.	engineered_region	1976	2016	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1956024	.	stop_codon	2009	2009	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1956024	.	polyA_site	2003	2016	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1956024	.	stem_loop	1983	2002	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1956024	.	CDS	1174	1854	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1956024	.	CDS	1174	1848	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1956024	.	engineered_tag	1855	1879	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1956024	.	promoter	948	1147	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1956024	.	start_codon	1120	1120	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1956024	.	CDS	948	1035	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1956024	.	non_covalent_binding_site	1036	1073	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1956024	.	engineered_region	948	1147	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1956024	.	promoter	1108	1113	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1956024	.	non_covalent_binding_site	1113	1147	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1956024	.	promoter	1084	1089	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1956024	.	engineered_region	83	939	.	+	0	ID=BBa_I13401;Name=BBa_I13401;Parent=BBa_K1956014
BBa_K1956024	.	terminator	899	939	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13401
BBa_K1956024	.	engineered_region	899	939	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1956024	.	stop_codon	932	932	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1956024	.	polyA_site	926	939	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1956024	.	stem_loop	906	925	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1956024	.	CDS	83	802	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13401
BBa_K1956024	.	CDS	83	802	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1956024	.	terminator	811	890	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13401
BBa_K1956024	.	engineered_region	811	890	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1956024	.	stem_loop	822	865	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1956024	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K1956014
BBa_K1956024	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1956024	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1956024	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1956024	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1956024	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1956024	.	ribosome_entry_site	64	76	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1956014
BBa_K1956024	.	ribosome_entry_site	70	73	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1956024	.	ribosome_entry_site	64	76	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1956024	.	engineered_region	64	76	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
>BBa_K1956024
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtac
tagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcaca
aattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaa
actacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatg
aaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacggga
actacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaa
agaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaa
aagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaac
aaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaaga
tcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaacta
tacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagc
aatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcg
ggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctc
gtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctcc
gaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaag
gtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgc
ttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctg
aaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccagg
actcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttat
gcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaa
atgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagc
tgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacga
acgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttata