##gff-version 3
##sequence-region BBa_K1956027 1 1995
BBa_K1956027	.	engineered_region	1	1995	.	+	0	ID=BBa_K1956018;Name=BBa_K1956018
BBa_K1956027	.	engineered_region	927	1995	.	+	0	ID=BBa_J04450;Name=BBa_J04450;Parent=BBa_K1956018
BBa_K1956027	.	promoter	927	1126	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1956027	.	engineered_region	927	1126	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1956027	.	promoter	1063	1068	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1956027	.	non_covalent_binding_site	1015	1052	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1956027	.	promoter	1087	1092	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1956027	.	CDS	927	1014	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1956027	.	non_covalent_binding_site	1092	1126	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1956027	.	start_codon	1099	1099	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1956027	.	terminator	1955	1995	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1956027	.	polyA_site	1982	1995	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1956027	.	stop_codon	1988	1988	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1956027	.	engineered_region	1955	1995	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1956027	.	stem_loop	1962	1981	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1956027	.	CDS	1153	1833	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1956027	.	CDS	1153	1827	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1956027	.	engineered_tag	1834	1858	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1956027	.	ribosome_entry_site	1135	1146	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1956027	.	conserved_region	1139	1142	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1956027	.	terminator	1867	1946	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1956027	.	stem_loop	1878	1921	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1956027	.	engineered_region	1867	1946	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1956027	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1956018
BBa_K1956027	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1956027	.	CDS	62	781	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K1956018
BBa_K1956027	.	CDS	62	781	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1956027	.	terminator	790	918	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1956018
BBa_K1956027	.	terminator	878	918	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1956027	.	polyA_site	905	918	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1956027	.	stop_codon	911	911	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1956027	.	engineered_region	878	918	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1956027	.	stem_loop	885	904	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1956027	.	terminator	790	869	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1956027	.	stem_loop	801	844	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1956027	.	engineered_region	790	869	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1956027	.	promoter	1	35	.	+	0	ID=BBa_J23117;Name=BBa_J23117;Parent=BBa_K1956018
>BBa_K1956027
ttgacagctagctcagtcctagggattgtgctagctactagagaaagaggagaaatactagatgcgtaaaggagaagaa
cttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagg
gtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaac
acttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaag
agtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaag
tcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttgg
acacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaac
ttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatg
gccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagacca
catggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccc
cgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaatt
aatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtga
gcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttc
atgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgt
acgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgca
gttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggt
ttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtg
agttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttg
ggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagac
ggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccg
acatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactc
caccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagt
cgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg
tgggcctttctgcgtttata