LOCUS       BBa_K1956046            1972 bp    DNA     linear       13-Mar-2025
DEFINITION  Dual fluorescence reporter
ACCESSION   BBa_K1956046
VERSION     BBa_K1956046.1  
FEATURES             Location/Qualifiers
     misc_feature    21..32
                     /label=B0034
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     promoter        1..32
                     /label=BBa_K525998
     CDS             39..758
                     /label=GFP protein
     CDS             39..758
                     /label=BBa_E0040
     stem_loop       778..821
                     /label=stem_loop
     misc_feature    767..846
                     /label=BBa_B0010
     terminator      767..846
                     /label=BBa_B0010
     stem_loop       862..881
                     /label=T7 TE
     misc_feature    888..888
                     /label=stop
     polyA_site      882..895
                     /label=polya
     misc_feature    855..895
                     /label=BBa_B0012
     terminator      855..895
                     /label=BBa_B0012
     misc_feature    39..895
                     /label=BBa_I13401
     CDS             904..991
                     /label=end of LacI coding region (inactive)
     misc_feature    904..1103
                     /label=BBa_R0010
     misc_feature    992..1029
                     /label=CAP binding site
     promoter        1040..1045
                     /label=-35
     promoter        1064..1069
                     /label=-10
     misc_feature    1069..1103
                     /label=LacI binding site
     misc_feature    1076..1076
                     /label=start
     promoter        904..1103
                     /label=BBa_R0010
     misc_feature    1116..1119
                     /label=conserved
     RBS             1112..1123
                     /label=BBa_B0034
     CDS             1130..1804
                     /label=mrfp1
     misc_feature    1811..1835
                     /label=Help:Barcodes
     CDS             1130..1810
                     /label=BBa_E1010
     stem_loop       1855..1898
                     /label=stem_loop
     misc_feature    1844..1923
                     /label=BBa_B0010
     terminator      1844..1923
                     /label=BBa_B0010
     stem_loop       1939..1958
                     /label=T7 TE
     misc_feature    1965..1965
                     /label=stop
     polyA_site      1959..1972
                     /label=polya
     misc_feature    1932..1972
                     /label=BBa_B0012
     terminator      1932..1972
                     /label=BBa_B0012
     misc_feature    904..1972
                     /label=BBa_J04450
     misc_feature    39..1972
                     /label=BBa_K1956016
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat gcgtaaagga gaagaacttt
       61 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
      121 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
      181 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg
      241 ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca
      301 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga
      361 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta
      421 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac
      481 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta
      541 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
      601 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
      661 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
      721 ggattacaca tggcatggat gaactataca aataataata ctagagccag gcatcaaata
      781 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
      841 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta
      901 gagcaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
      961 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     1021 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     1081 tgagcggata acaatttcac acatactaga gaaagaggag aaatactaga tggcttcctc
     1141 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
     1201 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
     1261 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
     1321 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
     1381 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
     1441 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
     1501 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
     1561 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg
     1621 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa
     1681 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca
     1741 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg
     1801 tgcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa
     1861 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1921 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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