##gff-version 3
##sequence-region BBa_K1956046 1 1972
BBa_K1956046	.	engineered_region	39	1972	.	+	0	ID=BBa_K1956016;Name=BBa_K1956016
BBa_K1956046	.	engineered_region	39	895	.	+	0	ID=BBa_I13401;Name=BBa_I13401;Parent=BBa_K1956016
BBa_K1956046	.	terminator	855	895	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13401
BBa_K1956046	.	engineered_region	855	895	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1956046	.	stop_codon	888	888	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1956046	.	polyA_site	882	895	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1956046	.	stem_loop	862	881	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1956046	.	terminator	767	846	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13401
BBa_K1956046	.	stem_loop	778	821	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1956046	.	engineered_region	767	846	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1956046	.	CDS	39	758	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13401
BBa_K1956046	.	CDS	39	758	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1956046	.	engineered_region	904	1972	.	+	0	ID=BBa_J04450;Name=BBa_J04450;Parent=BBa_K1956016
BBa_K1956046	.	terminator	1932	1972	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1956046	.	engineered_region	1932	1972	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1956046	.	stop_codon	1965	1965	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1956046	.	polyA_site	1959	1972	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1956046	.	stem_loop	1939	1958	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1956046	.	promoter	904	1103	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1956046	.	non_covalent_binding_site	992	1029	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1956046	.	engineered_region	904	1103	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1956046	.	non_covalent_binding_site	1069	1103	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1956046	.	start_codon	1076	1076	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1956046	.	promoter	1040	1045	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1956046	.	CDS	904	991	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1956046	.	promoter	1064	1069	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1956046	.	CDS	1130	1810	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1956046	.	CDS	1130	1804	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1956046	.	engineered_tag	1811	1835	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1956046	.	ribosome_entry_site	1112	1123	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1956046	.	conserved_region	1116	1119	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1956046	.	terminator	1844	1923	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1956046	.	stem_loop	1855	1898	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1956046	.	engineered_region	1844	1923	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1956046	.	promoter	1	32	.	+	0	ID=BBa_K525998;Name=BBa_K525998
BBa_K1956046	.	promoter	1	20	.	+	0	ID=annotation2129432;Name=T7 promoter;Parent=BBa_K525998
BBa_K1956046	.	ribosome_entry_site	24	27	.	+	0	ID=annotation2129433;Name=RBS;Parent=BBa_K525998
BBa_K1956046	.	engineered_region	21	32	.	+	0	ID=annotation2127788;Name=B0034;Parent=BBa_K525998
>BBa_K1956046
taatacgactcactatagggaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaat
tcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacgga
aaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatg
gtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgt
acaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgataccctt
gttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactata
actcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattga
agatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaac
cattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaa
cagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaat
gcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcatta
ggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatac
tagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatgga
aggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaa
ctgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagctt
acgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaa
cttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactg
cgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgt
acccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagt
taaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcc
cacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgat
agtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata