LOCUS       BBa_K195609              931 bp    DNA     linear       22-Nov-2024
DEFINITION  pRE with GFP generator
ACCESSION   BBa_K195609
VERSION     BBa_K195609.1  
FEATURES             Location/Qualifiers
     promoter        1..48
                     /label=BBa_K116603
     misc_feature    61..64
                     /label=conserved
     RBS             57..68
                     /label=BBa_B0034
     CDS             75..794
                     /label=GFP protein
     CDS             75..794
                     /label=BBa_E0040
     stem_loop       814..857
                     /label=stem_loop
     misc_feature    803..882
                     /label=BBa_B0010
     terminator      803..882
                     /label=BBa_B0010
     stem_loop       898..917
                     /label=T7 TE
     misc_feature    924..924
                     /label=stop
     polyA_site      918..931
                     /label=polya
     misc_feature    891..931
                     /label=BBa_B0012
     terminator      891..931
                     /label=BBa_B0012
ORIGIN
        1 agagcctcgt tgcgtttgtt tgcacgaacc atatgtaagt atttccttta ctagagaaag
       61 aggagaaata ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg
      121 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
      181 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
      241 catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag
      301 atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
      361 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag
      421 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
      481 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
      541 aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg
      601 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
      661 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
      721 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
      781 tatacaaata ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
      841 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
      901 tcaccttcgg gtgggccttt ctgcgtttat a
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