LOCUS       BBa_K195613              932 bp    DNA     linear       17-May-2024
DEFINITION  pCI with GFP generator
ACCESSION   BBa_K195613
VERSION     BBa_K195613.1  
FEATURES             Location/Qualifiers
     promoter        15..20
                     /label=-35
     promoter        25..41
                     /label=OR1
     promoter        1..17
                     /label=OR2
     promoter        38..43
                     /label=-10
     misc_feature    1..49
                     /label=BBa_R1051
     promoter        1..49
                     /label=BBa_R1051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..795
                     /label=GFP protein
     CDS             76..795
                     /label=BBa_E0040
     stem_loop       815..858
                     /label=stem_loop
     misc_feature    804..883
                     /label=BBa_B0010
     terminator      804..883
                     /label=BBa_B0010
     stem_loop       899..918
                     /label=T7 TE
     misc_feature    925..925
                     /label=stop
     polyA_site      919..932
                     /label=polya
     misc_feature    892..932
                     /label=BBa_B0012
     terminator      892..932
                     /label=BBa_B0012
ORIGIN
        1 taacaccgtg cgtgttgaca attttacctc tggcggtgat actggttgct actagagaaa
       61 gaggagaaat actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt
      121 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
      181 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
      241 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca
      301 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      361 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
      421 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
      481 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
      541 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
      601 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
      661 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
      721 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
      781 ctatacaaat aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      841 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      901 ctcaccttcg ggtgggcctt tctgcgttta ta
//